A novel and one-pot synthesis of new 1-tosyl pyrrol-2-one derivatives and analysis of carbonic anhydrase inhibitory potencies

Characterization of carbonic anhydrase from Turkish native “Gerze” chicken and influences of metal ions on enzyme activity

“…The optimum pH was found to be 8.0. The result is similar to CAs obtained from human erythrocytes 23,24 . The enzyme was seen to exhibit the highest activity at 25 C in a study of temperatures between 5 and 70 C. The optimum ionic strength of the enzyme was estimated to be 10 mM Tris-HCl buffer.…”

Interactions of Fungicides and Pesticides with Specific Enzymes

“…For instance, medical drugs (Alici et al, 2008;Ekinci et al, 2007a ), metal ions (Ekinci et al, 2007b;Tekman et al, 2008), pesticides and fungicides Ceyhun et al, 2010a) generally inhibit the enzymes at very low concentrations (Ekinci & Beydemir, 2010a). These inhibitions could be very dangerous in some cases (Bayram et al, 2008;Senturk et al, 2009a,b;Coban et al, 2009;Alp et al, 2010). Although investigation of the alterations in the activity of several enzymes have gained considerable attention over the past years (Coban et al, 2007;, there is still a deep need of understanding the influences of pesticides and fungicides on specific enzyme systems.…”

Kinetic andin silicostudies of hydroxy-based inhibitors of carbonic anhydrase isoforms I and II

“…It is interesting to note that the compounds 7-12 and 15 were much better hCA I inhibitors as compared to the corresponding compounds 13 and 14. Kinetic investigations (Lineweaver-Burk plots, data not shown) indicate that similarly to sulfonamides and inorganic anions [5][6][7][8][9][10][11][12][25][26][27][28][29][30] , all the investigated natural compounds act as competitive inhibitors with 4-NPA as substrate (i.e. they bind in different regions of the active site cavity as compared to the substrate).…”