A novel and homogeneous scaffold material: preparation and evaluation of alginate/bacterial cellulose nanocrystals/collagen composite hydrogel for tissue engineering

Yan, Huiqiong; Huang, Denggao; Chen, Xiuqiong; Liu, Haifang; Ye, Feng; Zhao, Zhendong; Dai, Zihao; Zhang, Xueqin; Lin, Qiang

doi:10.1007/s00289-017-2077-0

Cited by 59 publications

(38 citation statements)

References 44 publications

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“…Design of hydrogels on the macroscopic level assumes control over the size and porous structure of the gels [70]. Hydrogel matrices can be either non-porous (having only small pores that are typically in the range of tens of nm for the alginate gel network [71]) or contain macroscopic pores that are typically in the range of 10–500 μm [72] (Figure 3). Dual nano- and macro-porosity is essential for controlled growth of a tissue and drug delivery [70].…”

Section: Ca2+-alginate Gel Based Scaffoldsmentioning

confidence: 99%

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Porous Alginate Scaffolds Assembled Using Vaterite CaCO3 Crystals

2019

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Formulation of multifunctional biopolymer-based scaffolds is one of the major focuses in modern tissue engineering and regenerative medicine. Besides proper mechanical/chemical properties, an ideal scaffold should: (i) possess a well-tuned porous internal structure for cell seeding/growth and (ii) host bioactive molecules to be protected against biodegradation and presented to cells when required. Alginate hydrogels were extensively developed to serve as scaffolds, and recent advances in the hydrogel formulation demonstrate their applicability as “ideal” soft scaffolds. This review focuses on advanced porous alginate scaffolds (PAS) fabricated using hard templating on vaterite CaCO3 crystals. These novel tailor-made soft structures can be prepared at physiologically relevant conditions offering a high level of control over their internal structure and high performance for loading/release of bioactive macromolecules. The novel approach to assemble PAS is compared with traditional methods used for fabrication of porous alginate hydrogels. Finally, future perspectives and applications of PAS for advanced cell culture, tissue engineering, and drug testing are discussed.

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Section: Ca2+-alginate Gel Based Scaffoldsmentioning

confidence: 99%

“…Reproduced with permission from [71], published by John Wiley & Sons, 2016; (d,e) — porous alginate scaffold. Reproduced with permission from [72], published by Springer-Verlag Berlin Heidelberg, 2018.…”

Section: Figurementioning

confidence: 99%

Porous Alginate Scaffolds Assembled Using Vaterite CaCO3 Crystals

2019

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“…Alginate is a polymer extracted from natural sources, widely used in the pharmaceutical area and in tissue engineering. This material has several advantages, including the activation of the immune system, nontoxicity, biocompatibility and biodegradability (9)(10)(11). In our research, in order to reinforce and cover the system with alginate, it was applied bacterial cellulose nanocrystals (NCC).…”

Section: Introductionmentioning

confidence: 99%

“…NCC are formed from the action of cellulase in contact with bacterial cellulose. This material has a renewable and sustainable origin, with a good costbene t, good biocompatibility and biodegradability, besides presenting a good mechanical resistance, large surface area, low toxicity (10,14,15).…”

Section: Introductionmentioning

confidence: 99%

Alginate-amphothericin B Nanocomplexes Covered by Nanocrystals From Bacterial Cellulose: Physico-chemical Characterization and in Vitro Toxicity

Vs¹,

Silva-Carvalho²,

Martins³

et al. 2020

Preprint

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Background: Nanostructured systems free of surfactants have pharmacotechnical and biopharmaceutical advantages, in addition to being a green process. Amphotericin B (AmB) is a drug with anti-leishmanial and anti-fungal potential, but its low water solubility and permeability limit its therapeutic use. Therefore, it could profit from being incorporated into nanostructured systems. In the present study, a self-assembled nanocomplex of alginate (Alg), a polysaccharide extracted from natural sources that can be used in the pharmaceutical area, with AmB was produced in order to improve the limited therapeutic use of this drug (Alg-AmB). Further, as a reinforcing component, cellulose nanocrystals (NCC) were ionically adsorbed into the surface of the nanocomplex systems (Alg-AmB + NCC). Results: Despite some polydispersity (0.523 ± 0.073), this straightforward process allowed to obtain water soluble particles with a hydrodynamic size of 258.87 ± 10.41 nm and charge of -62.93 ± 2.02 mV. Furthermore, the ionic adsorption of the NCC into the Alg-AmB nanocomplex surface was confirmed by an increase in the particle size (466.3 ± 17.57 nm) and a small surface charge decrease (-55.75 ± 1.23 mV). The amorphous inclusion complex of AmB into the polysaccharide chain network in both formulations was confirmed by DSC and FTIR. AmB in the nanocomplexes was in supper-aggregated form and showed good biocompatibility, being significantly less cytotoxic in vitro against kidney cells and significantly less hemolytic comparatively to the free-drug. Conclusions: The results were indicated the Alg-AmB nanocomplex can be considered an economical, non-toxic alternative to improve the AmB therapeutic effect. Furthermore, NCC coating of the nanocomplexes brought additional protection to the system without compromised the advantages attributed to the developed formulation.

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“…For the ECM application, materials should have a porous structure with adequate mechanical strength. Besides, biocompatibility and degradation potentials are critical parameters for the ECM [2][3][4]. Several techniques are used for the fabrication of scaffolds for tissue engineering applications.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Biocompatibility of Electrospun Poly(ε-Caprolactone)/Cellulose Nanocrystals-Nanofibers for Tissue Engineering

Dutta

Patel

Seo

et al. 2019

Journal of Nanomaterials

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Cellulose nanocrystals (CNCs) have emerged as promising materials for the fabrication of micro/nanoplatforms that can replace tissues more effectively. CNCs offer interesting properties that facilitate the enhancement of polymer properties. Cytotoxicity of rice husk-derived CNCs was evaluated through WST-1 assay in the presence of human mesenchymal stem cells. Electrospinning technique was used to fabricate nanofibers of poly-ε-caprolactone and its composites. Significant improvement in the mechanical property was observed in the composites relative to the pure polymer. This improvement was attributed to the better interfacial interactions between the polymer matrix and CNCs. Notably, better cell viability and differentiation were observed with the composite nanofibers than with the pure polymers. The osteogenic potential of the fabricated nanofibers was assessed by alizarin red S staining and real-time PCR. Enhanced mineralization occurred in the presence of the composite rather than pure polymer nanofibers. Furthermore, the higher levels of osteogenic markers observed with the media containing the composites clearly indicated their osteogenic potential. These results suggested that fabricated composites have the potential to be used as a biomaterial for tissue engineering applications.

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A novel and homogeneous scaffold material: preparation and evaluation of alginate/bacterial cellulose nanocrystals/collagen composite hydrogel for tissue engineering

Cited by 59 publications

References 44 publications

Porous Alginate Scaffolds Assembled Using Vaterite CaCO3 Crystals

Porous Alginate Scaffolds Assembled Using Vaterite CaCO3 Crystals

Alginate-amphothericin B Nanocomplexes Covered by Nanocrystals From Bacterial Cellulose: Physico-chemical Characterization and in Vitro Toxicity

In Vitro Biocompatibility of Electrospun Poly(ε-Caprolactone)/Cellulose Nanocrystals-Nanofibers for Tissue Engineering

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