A novel and efficient protocol for the isolation of genomic DNA from&lt;br&gt;mulberry (Morus L.)

Anuradhabr, H; Vijayanbr, Kunjupillai; Manjula, C.P.

doi:10.9755/ejfa.v25i2.11660

Cited by 16 publications

(18 citation statements)

References 15 publications

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“…Based on the results of this expriment, the mature leaf can be an alternative to extract DNA although it was known to contain many secondary metabolites and polysaccharides. This result was in accordance with that of Small et al (2004), Chathrath et al (2013) and Anuradha et al (2013).…”

Section: Resultssupporting

confidence: 92%

AN EFFECTIVE METHOD FOR DNA EXTRACTION OF MATURE LEAF OF SAPODILLA (Manilkara zapota (L.) van Royen)

Sari

Murti

2015

Agrivita.J.Agr.Sci

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Perennial crop leaves contain polysaccharides, polyphenols, and other secondary metabolites in high concentration. The presence of those compounds inhibit enzymatic activities and amplification of DNA. The existing extraction methods were not able to dissociate the metabolites contaminants of sapodilla (Manilkara zapota (L.) van Royen) leaves and thus resulting in low quality of extracted DNA. The aim of this experiment was to develop an effective method to extract DNA from mature leaf samples of sapodilla (Manilkara zapota (L.) van Royen). Fifth modification of Doyle & Doyle DNA extraction protocol with modified concentration of buffer reagent (consisted of: CTAB 2.8%; NaCl 2.5M; mercaptoethanol 3%, and PVP 2.5%) and repetition of some phase purification (liquid nitrogen; three times CIAA; two times ethanol 70%, RNAse 1µl) generated high quality DNA and clear band of PCR amplification using RAPD primers.

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Section: Resultssupporting

confidence: 92%

AN EFFECTIVE METHOD FOR DNA EXTRACTION OF MATURE LEAF OF SAPODILLA (Manilkara zapota (L.) van Royen)

Sari

Murti

2015

Agrivita.J.Agr.Sci

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“…To provide a reliable molecular analysis in plants, the conditions of DNA extraction should be tested once a standard protocol applicable to all plant species remains unavailable (Tacuatiá et al, 2012;Anuradha et al, 2013). Therefore, studies of genetic diversity, population structure, phylogenetics, and inheritance patterns of plants usually start from developing a suitable method of DNA extraction for a species or group of species (Akkurt, 2012;Li et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Research Article An optimized protocol for DNA extraction in plants with a high content of secondary metabolites, based on leaves of Mimosa tenuiflora (Willd.) Poir. (Leguminosae).

Arruda¹,

Pereira²,

Silva-Castro³

et al. 2017

Genet. Mol. Res.

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Some species are characterized by a high content of tannins, alkaloids, and phenols in their leaves. These secondary metabolites are released during DNA extraction and might hinder molecular studies based on PCR (polymerase chain reaction). To provide an efficient method to extract DNA, Mimosa tenuiflora, an important leguminous plant from Brazilian semiarid region used in popular medicine and as a source of fuelwood or forage, was used. Eight procedures previously reported for plants were tested and adapted from leaf tissues of M. tenuiflora stored at -20°C. The optimized procedure in this study encompassed the utilization of phenol during deproteinization, increased concentrations of cetyltrimethylammonium bromide and sodium chloride, and a shorter period and lower temperature of incubation concerning other methods. The extracted DNA did not present degradation, and amplification via PCR was successful using ISSR, trnL, ITS, and ETS primers. Besides M. tenuiflora, this procedure was also tested and proved to be efficient in genetic studies of other plant species.

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“…The purity of DNA is one of the major factors affecting the success of genomic or genetic technologies studies. Nucleic acid isolation from polyphenol rich plants fail to produce good quality DNA or RNA as polyphenols adhere and interfere with DNA during isolation (Anuradha et al, 2013). For quantity setting of the extracted DNA, Nanodrop Nanophotometer™ was used.…”

Section: Methodsmentioning

confidence: 99%

Using of Inter Microsatellite Polymorphism to evaluate gamma-irradiated<br>Amaranth mutants

iarovsk¹,

Labajov²

2013

Emir. J. Food Agric

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Molecular markers ISSR were used to analyse γ-irradiated mutants of amaranth Ficha cultivar and K-433 hybrid; tested six microsatellite -primed PCR markers. First analysis of gamma-irradiated amaranth mutant lines was done using the ISSR. In the study, primers that distinguish the mutant lines from control plant were reported as well as primer with the differentiation ability for all analysed mutant lines. The very specific changes in the mutant lines´ non-coding regions based on inter microsatellite length polymorphism were analysed. Mutant lines of the Ficha cultivar (C15, C26, C27, C82, C236) shared a genetic dissimilarity of 0.16 and their ISSR profiles were more similar to the Ficha than those of K-433 hybrid mutant lines. The K-433 mutant lines (D54, D279, D282) shared genetic dissimilarity of 0.22 but are more distinct to their control plant as a whole, as those of the Ficha mutant lines. Different ISSR fingerprints patters of the mutant lines when compared to the Ficha cultivar and K-433 hybrid ISSR specific profiles may be part of a consequence of the complex response of the intergenic space of mutant lines to the gamma-radiance.

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A novel and efficient protocol for the isolation of genomic DNA from<br>mulberry (Morus L.)

Cited by 16 publications

References 15 publications

AN EFFECTIVE METHOD FOR DNA EXTRACTION OF MATURE LEAF OF SAPODILLA (Manilkara zapota (L.) van Royen)

AN EFFECTIVE METHOD FOR DNA EXTRACTION OF MATURE LEAF OF SAPODILLA (Manilkara zapota (L.) van Royen)

Research Article An optimized protocol for DNA extraction in plants with a high content of secondary metabolites, based on leaves of Mimosa tenuiflora (Willd.) Poir. (Leguminosae).

Using of Inter Microsatellite Polymorphism to evaluate gamma-irradiated<br>Amaranth mutants

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