“…However, high endogenous levels of polysaccharides and polyphenols interfere with the isolation of good quality DNA, thereby rendering it unsuitable for downstream analyses [[1], [2], [3]]. During cell disruption, phenolic compounds come out of the vacuoles, become readily oxidized and irreversibly bind with nucleic acids and proteins, thus resulting in a dark DNA pellet unsuitable for most enzymatic manipulations [4]. On the other hand, the viscous nature of polysaccharides makes extracted DNA fractious to pipetting; and, it also interferes with various biological enzymes, and especially hinders the PCR reaction by inhibiting the Thermus aquaticus DNA polymerase ( Taq.…”