A novel amperometric phenol biosensor based on immobilized HRP on poly(glycidylmethacrylate)-grafted iron oxide nanoparticles for the determination of phenol derivatives

Çevik, Emre; Şenel, Mehmet; Baykal, A.; Abasıyanık, M. Fatih

doi:10.1016/j.snb.2012.07.026

Cited by 54 publications

(29 citation statements)

References 48 publications

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“…Using a neodymium magnet located at the bottom part of the GC disk, Fe 3 O 4 @pDA/HRP MNPs were magnetically captured in a reproducible way. The electrochemical detection of phenolic compounds was achieved thanks to the ability of such compounds to re-oxidized the HRP enzyme, acting as electron shuttles from the redox centre of the HRP molecules to the GCE surface in the presence of H 2 O 2 following the double displacement or "ping-pong" mechanism [40][41][42][43][44][45][46][47] .…”

Section: Electrochemical Characterization Of Fe 3 O 4 @Pda/hrp Mnpsmentioning

confidence: 99%

“…In addition, traditional electrochemical sensors are very poor and the electrode surface may be fouled by insulating polymer films or by-products generated during phenolic detection 36,37 . Owing to those disadvantages, researchers have focused on the use on nanostructured and catalytic materials 31,38,39 or in enzyme based amperometric biosensors [40][41][42][43][44][45][46][47] .…”

Section: Introductionmentioning

confidence: 99%

“…MNPs, synthesized by co-precipitation method, were coated with poly(dopamine) film forming a core-shell polymeric-Fe 3 O 4 MNPs structure (Fe 3 O 4 @pDA MNPs) where HRP was immobilized. Although, HRP was immobilized using different approaches, few reports have been described in the literature using pDA as immobilization matrix [40][41][42][43][44][45][46][47] . The modified MNPs were characterized using different techniques such as XPS, AFM, FTIR, X-ray and the electroanalytical performance of the as prepared biosensor were evaluated against some common phenolic compounds.…”

Section: Introductionmentioning

confidence: 99%

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Amperometric magnetobiosensors using poly(dopamine)-modified Fe₃O₄ magnetic nanoparticles for the detection of phenolic compounds

et al. 2015

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aThe synthesis of poly(dopamine)-modified magnetic nanoparticles (MNPs) and their application to prepare electrochemical enzyme biosensor useful to detect phenolic compounds is reported in this work. MNPs of about 16 nm were synthetized by co-precipitation method and conveniently modified with poly(dopamine). Non-modified and modified MNPs were characterized using X-ray photoelectron spectroscopy (XPS), Raman and infrared spectroscopy, X-ray diffraction (XRD) and atomic force microscopy (AFM). Horseradish peroxidase (HRP) was covalently immobilized onto the surface of the poly(dopamine)-modified MNPs via Michael addition and/or Schiff base formation and used to construct a biosensor for phenolic compounds by capturing the HRP-modified-nanoparticles onto the surface of a magnetic-modified glassy carbon electrode (GCE). Cyclic voltammetry and amperometry were used to study the electrochemical and analytical properties of the biosensor using hydroquinone (HQ) as redox probe. Among different phenolic compounds studied the biosensor exhibited higher sensitivity for HQ, 1.38 A M −1 cm −2, with limits of detection and quantification of 0.3 and 1.86 µM, respectively. The analytical biosensor performance for HQ and 2-aminophenol compared advantageously with previous phenolic biosensors reported in the literature.

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Section: Electrochemical Characterization Of Fe 3 O 4 @Pda/hrp Mnpsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Amperometric magnetobiosensors using poly(dopamine)-modified Fe₃O₄ magnetic nanoparticles for the detection of phenolic compounds

et al. 2015

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“…7 However, these techniques are known to be expensive, require sample pre-treatment and are not adequate for in situ analysis. 8 Therefore the analytical methods used for phenol detection which are rapid and simple have come into preference when compared to the more sophisticated ones.…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination of Different Phenolic Compounds Using Electrochemical Biosensor and Multivariate Calibration

Dantas

Nogueira

Etchegaray

et al. 2017

J. Braz. Chem. Soc.

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Phenolic compounds are important environmental contaminants due to their high toxicity and persistence in the environment. The use of enzyme-based electrochemical biosensors is a simple, sensitive and low-cost alternative for the determination of these pollutants in contaminated waters. However, in most cases, it is impossible to detect specific compounds in a mixture of phenols due to signal-overlap, as the instruments operate at very close potentials, given that the system is based on a single enzyme to detect similar structures. In order to overcome this problem, in the present work we have successfully used multivariate calibration with partial least squares (PLS) for the simultaneous determination of hydroquinone and guaiacol by a tyrosinase-based biosensor that was assembled using an enzyme extract from yam. The use of PLS allowed us to work with a large number of voltammograms, leading to a single mathematical model for the simultaneous determination of phenols of similar structure in real samples with concentration values of mmol L -1 .

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“…[25] The detection limit was 3.3 µM. Fe 3 O 4 nanoparticles in a poly(glycidylmethacrylate)/ horseradish peroxidase based gold electrode were reported for phenol by Çevik et al [41] They reported a detection limit of 500 µM even though they used metallic nanoparticles to accelerate the electron transfer rate. The biosensor sensitivity was 3 nA/µM across the linear concentration range obtained in our work.…”

Section: Optimization Of Flow Rate Studies and Phenols On The Responsementioning

confidence: 98%

Electrochemical, continuous-flow determination ofp-benzoquinone on a gold nanoparticles poly(propylene-co-imidazole) modified gold electrode

Korkut

Uzunçar

Kilic

et al. 2016

Instrumentation Science & Technology

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A novel continuous flow biosensor based on gold nanoparticles and poly(propylene-co-imidazole) was developed for the online determination of p-benzoquinone. The amperometric response was measured as a function of p-benzoquinone concentration at an applied potential of À 50 mV. The hydrogen peroxide concentration was optimized and fixed at 1 mM in samples. The mass transfer resistance of the copolymer film was minimized, and the flow cell was regenerated quickly at 1 mL/min. The resulting device provided good analytical performance based on a linear dynamic range from 5-100 µM, a short response time of 3 s, a detection limit of 3.3 µM, excellent repeatability with a relative standard deviation of 0.82%, long-term stability of 95% after four weeks, and an accuracy of 105%. The gold nanoparticles enhanced the electron transfer rate on the electrode. The apparent Michaelis-Menten constant was 4 mM, showing that the enzyme retained catalytic specificity and provided high activity for p-benzoquinone.

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A novel amperometric phenol biosensor based on immobilized HRP on poly(glycidylmethacrylate)-grafted iron oxide nanoparticles for the determination of phenol derivatives

Cited by 54 publications

References 48 publications

Amperometric magnetobiosensors using poly(dopamine)-modified Fe₃O₄ magnetic nanoparticles for the detection of phenolic compounds

Amperometric magnetobiosensors using poly(dopamine)-modified Fe₃O₄ magnetic nanoparticles for the detection of phenolic compounds

Simultaneous Determination of Different Phenolic Compounds Using Electrochemical Biosensor and Multivariate Calibration

Electrochemical, continuous-flow determination ofp-benzoquinone on a gold nanoparticles poly(propylene-co-imidazole) modified gold electrode

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A novel amperometric phenol biosensor based on immobilized HRP on poly(glycidylmethacrylate)-grafted iron oxide nanoparticles for the determination of phenol derivatives

Cited by 54 publications

References 48 publications

Amperometric magnetobiosensors using poly(dopamine)-modified Fe3O4 magnetic nanoparticles for the detection of phenolic compounds

Amperometric magnetobiosensors using poly(dopamine)-modified Fe3O4 magnetic nanoparticles for the detection of phenolic compounds

Simultaneous Determination of Different Phenolic Compounds Using Electrochemical Biosensor and Multivariate Calibration

Electrochemical, continuous-flow determination ofp-benzoquinone on a gold nanoparticles poly(propylene-co-imidazole) modified gold electrode

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Amperometric magnetobiosensors using poly(dopamine)-modified Fe₃O₄ magnetic nanoparticles for the detection of phenolic compounds

Amperometric magnetobiosensors using poly(dopamine)-modified Fe₃O₄ magnetic nanoparticles for the detection of phenolic compounds