Electrochemical, continuous-flow determination ofp-benzoquinone on a gold nanoparticles poly(propylene-co-imidazole) modified gold electrode

Abstract

A novel continuous flow biosensor based on gold nanoparticles and poly(propylene-co-imidazole) was developed for the online determination of p-benzoquinone. The amperometric response was measured as a function of p-benzoquinone concentration at an applied potential of À 50 mV. The hydrogen peroxide concentration was optimized and fixed at 1 mM in samples. The mass transfer resistance of the copolymer film was minimized, and the flow cell was regenerated quickly at 1 mL/min. The resulting device provided good analytical performance based on a linear dynamic range from 5-100 µM, a short response time of 3 s, a detection limit of 3.3 µM, excellent repeatability with a relative standard deviation of 0.82%, long-term stability of 95% after four weeks, and an accuracy of 105%. The gold nanoparticles enhanced the electron transfer rate on the electrode. The apparent Michaelis-Menten constant was 4 mM, showing that the enzyme retained catalytic specificity and provided high activity for p-benzoquinone.

Enhanced performances of sensors based on screen printed electrodes modified with nanosized NiO particles

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Novel Enzymatic Rhodium Modified Poly(styrene‐g‐oleic amide) Film Electrode for Hydrogen Peroxide Detection

“…The biosensor sensitivity was found to be 57 μA mM −1 cm −2 which was higher than the previously published hydrogen peroxide sensors based on horseradish peroxidase or non‐enzymatic electrodes (Supporting Information‐Table S1). The measurement accuracy of the biosensor was 96 % calculated according to the formula given as (measured concentration/actual concentration)×100 . Repeatability of the detection in one operation was monitored by the measurement of the response for a serie of 6 succesive additions of 2 mM hydrogen peroxide into 100 mM phosphate buffer (pH 7) at −0.65 V (vs. Ag/AgCl).…”

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