A novel amperometric method for antioxidant activity determination using DPPH free radical

Methods for Total Antioxidant Activity Determination: A Review

“…The developed method was applied for the evaluation of antioxidant activity of some water or ethanol soluble pure antioxidant compounds and of several samples of tea, wine and some other beverages. The good correlation of results (R 2 =0.9993) expressed as Trolox equivalents, was obtained between the proposed amperometric method and the classic spectroscopic method [96].…”

“…The amperometric determination of the antioxidant activity [96] was based on the reduction of 2,2-diphenyl-1-picrylhydrazyl (DPPH•) at the glassy carbon electrode. All the experiments were performed in a three-electrode electrochemical cell at 140 mV vs. Hg 2 Cl 2 | 3M KCl using ethanolic solution (40%) and 0.033M KCl in 0.033 M phosphate buffer, pH=7.4.…”

Methodological aspects about in vitro evaluation of antioxidant properties

“…In addition, the spectrophotometric measurements can be affected by compounds, such as carotenoids, that absorb at the wavelength of determination as well as by the turbidity of the sample. In this way, the electrochemical detection proposed by Milardovic et al [195,196] [202]. This approach allowed a considerable reduction of the time taken for a single analysis for samples containing or originating slow reacting antioxidant compounds.…”

“…The scavenging capacity is generally evaluated in organic media by monitoring the absorbance decrease at 515-528 nm until the absorbance remains constant [194] or by electron spin resonance [74]. Recently, the determination of antioxidant capacity based on the amperometric reduction of DPPH • at the glassy carbon electrode was proposed by Milardovic et al [195]. The resulting current on a glassy carbon electrode polarized at fixed potential was proportional to the residual concentration of DPPH • after reaction with the antioxidant(s).…”

New Approach for Measuring Antioxidant Activity Via a Graphite Sensor

“…Recently, a number of DPPH• -based tests for the assessment of antioxidant activity have been developed. The evaluation of antioxidant capacity based on the amperometric reduction of DPPH• at the glassy carbon electrode was reported [14,15], TLC [16], HPLC techniques [17,18,19,20] and flow injection based methods for determination of scavenging capacity against DPPH• were also reported [21,22,23,24,25]. A DPPH• based optical sensor for screening of antioxidant activity was introduced by Steinberg et al [26].…”