A novel allotetraploid gibel carp strain with maternal body type and growth superiority

Genetic identification of a newly synthetic allopolyploid strain with 206 chromosomes in polyploid gibel carp

“…The fin tissues were stored in 95% ethanol at −20°C for DNA extraction and blood cells were fixed by 70% ethanol and stored at 4°C for DNA content measurement. Other 100 synthetic allopolyploid individuals and 100 gibel carp A + individuals were collected to measure phenotype traits including total length (TL), body height (BH), head length (HL) and body weight (BW) to distinguish the two gibel carp strains (Li, Liang et al., ).…”

“…Red blood cells were collected and treated to measure the DNA content by flow cytometer (BeckmanCoulter Cell Lab QuantaTM SC) as described (Li, Liang et al., ; Wang et al., ). Then the ploidy of allopolyploid strain was determined by comparing the DNA content with clone A + and common carp as control.…”

“…The preparation of metaphase chromosomes was performed as described (Zhu & Gui, ; Li, Zhang et al., ). A total of the best 100 metaphase spreads were detected and the statistics modal number was characterized as the chromosome number (Li, Liang et al., ; Liu et al., ; Wang et al., ; Zhou & Gui, ).…”

“…The qualities of extracted DNA were valued by Nanodrop 2000 and then diluted to appropriate concentration for PCR reaction. The primers and the amplification procedure of Tf and ITS1were referred to previous reports (Li, Liang et al., ; Li et al., ; Wang et al., ; Yang & Gui, ; Zhu, Zhong, Zhang, Liu & Li, ). A total of six synthetic allopolyploid individuals, one gibel carp clone A + individual and one red common carp individual were selected to identify TF and ITS1 alleles.…”

“…Intriguingly, a few of allopolyploid individuals (about 0.5%) with an extra chromosome set of common carp were found from the allogynogenetic populations (Fan et al., ; Gui, Liang, Zhu & Jiang, , ; Zhao, Liu, Chen, Qing & Dong, ). These findings implicate that eggs of polyploid gibel carp not only have the capability to maintain all chromosomes itself, but also possess the ability to form higher ploidy allopolyploids by incorporating an alien sperm chromosome set (Gui, ; Gui & Zhou, ; Li, Liang, Wang, Zou & Gui, ; Yang, Gui, Zhu, Liang & Jiang, ; Yi et al., ; Zhang et al., ). Moreover, several allopolyploid clones or strains have been synthetic because they still maintain unisexual gynogenesis ability (Gui & Zhou, ; Zhu & Gui, ), and some of them have been applied to aquaculture practice because of the growth superiority (Li, Liang, et al., ).…”