A novel aflatoxin B1 biosensor based on a porous anodized alumina membrane modified with graphene oxide and an aflatoxin B1 aptamer

Mo, Rijian; He, Lei; Yan, Xiemin; Su, Tiantian; Zhou, Chunxia; Wang, Zhe; Hong, Pengzhi; Sun, Song; Li, Chengyong

doi:10.1016/j.elecom.2018.08.012

Cited by 55 publications

(17 citation statements)

References 41 publications

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“…An interesting AFB1 biosensor [64] is assembled by using a porous anodized alumina membrane modified with graphene oxide and an aptamer of AFB1. Briefly, the aptamer is immobilized on the surface of the porous anodized alumina nanochannels by covalent bonding.…”

Section: Toxinsmentioning

confidence: 99%

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

Curulli

2021

Preprint

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Safety and quality are key issues for the food industry. Consequently, there is a growing demand to preserve the food chain and products against substances toxic, harmful to human health such as contaminants, allergens, toxins, or pathogens. For this reason, it is mandatory to develop highly sensitive, reliable, rapid, and cost-effective sensing systems/devices such as electrochemical sensors/biosensors. Generally, conventional techniques are limited by long time of analyses, expensive and complex procedures, and they require skilled personnel. Therefore, the development of performant electrochemical biosensors can significantly support the screening of food chain and products. Here, we report some of the recent developments in this area and analyze the contributions produced by electrochemical biosensors in the food screening and the challenges to address.

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Section: Toxinsmentioning

confidence: 99%

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

Curulli

2021

Preprint

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“…Nanochannel sensors are sensitive to electric charge change. Mo et al (2018) developed novel sandwich structures of electrochemical aptasensors using a PAA membrane, aptamer, and GO to monitor AFB1. In the presence of AFB1, GO detached from the nanochannel surfaces, causing a decrease in the steric effect and charge density.…”

Section: Electrochemical Aptasensorsmentioning

confidence: 99%

Progress on Structured Biosensors for Monitoring Aflatoxin B1 From Biofilms: A Review

Wang

Yang

2020

Front. Microbiol.

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Aspergillus exists commonly in many crops and any process of crop growth, harvest, storage, and processing can be polluted by this fungus. Once it forms a biofilm, Aspergillus can produce many toxins, such as aflatoxin B1 (AFB1), ochratoxin, zearalenone, fumonisin, and patulin. Among these toxins, AFB1 possesses the highest toxicity and is labeled as a group I carcinogen in humans and animals. Consequently, the proper control of AFB1 produced from biofilms in food and feed has long been recognized. Moreover, many biosensors have been applied to monitor AFB1 in biofilms in food. Additionally, in recent years, novel molecular recognition elements and transducer elements have been introduced for the detection of AFB1. This review presents an outline of recent progress made in the development of biosensors capable of determining AFB1 in biofilms, such as aptasensors, immunosensors, and molecularly imprinted polymer (MIP) biosensors. In addition, the current feasibility, shortcomings, and future challenges of AFB1 determination and analysis are addressed.

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“…Mo and colleagues have prepared a probe for the detection of aflatoxin B1, which used graphene oxide and an aflatoxin B1 aptamer-modified multi-anodized anodized aluminum ( Figure 5) [22]. First, the aptamer was attached onto porous anodized aluminum nanopores through covalent attachment.…”

Section: Electrochemical Aptamer Sensor For Afb1mentioning

confidence: 99%

Recent Development of Aptamer Sensors for the Quantification of Aflatoxin B1

et al. 2019

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Aflatoxin B1 (AFB1) is one of the most frequently-found mycotoxins in contaminated food. As the content of mycotoxins is particularly low in food, the development of probes to detect AFB1 in foods with high sensitivity and selectivity is an urgent social need for the evaluation of food quality. Numerous techniques have been developed to monitor AFB1. Nevertheless, most of them require cumbersome, labor-consuming, and sophisticated instruments, which have limited their application. An aptamer is a single, short nucleic acid sequence that is capable of recognizing different targets. Owing to their unique properties, aptamers have been considered as alternatives to antibodies. Aptasensors are considered to be an emerging strategy for the quantification of aflatoxin B1 with high selectivity and sensitivity. In this review, we summarize recent developments in colormetric, electrochemical, SERS, and fluorescent aptasensors for the quantification of AFB1. Finally, the perspectives and current challenges of aptasensors for AFB1 are outlined.

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A novel aflatoxin B1 biosensor based on a porous anodized alumina membrane modified with graphene oxide and an aflatoxin B1 aptamer

Cited by 55 publications

References 41 publications

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

Electrochemical Biosensors in Food Safety: Challenges and Perspectives

Progress on Structured Biosensors for Monitoring Aflatoxin B1 From Biofilms: A Review

Recent Development of Aptamer Sensors for the Quantification of Aflatoxin B1

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