Recent Development of Aptamer Sensors for the Quantification of Aflatoxin B1

Abstract

Aflatoxin B1 (AFB1) is one of the most frequently-found mycotoxins in contaminated food. As the content of mycotoxins is particularly low in food, the development of probes to detect AFB1 in foods with high sensitivity and selectivity is an urgent social need for the evaluation of food quality. Numerous techniques have been developed to monitor AFB1. Nevertheless, most of them require cumbersome, labor-consuming, and sophisticated instruments, which have limited their application. An aptamer is a single, short nucleic acid sequence that is capable of recognizing different targets. Owing to their unique properties, aptamers have been considered as alternatives to antibodies. Aptasensors are considered to be an emerging strategy for the quantification of aflatoxin B1 with high selectivity and sensitivity. In this review, we summarize recent developments in colormetric, electrochemical, SERS, and fluorescent aptasensors for the quantification of AFB1. Finally, the perspectives and current challenges of aptasensors for AFB1 are outlined.

Adverse Effects, Transformation and Channeling of Aflatoxins Into Food Raw Materials in Livestock

“…The different methods can be characterized by several parameters such as accuracy, applicability, reproducibility, limit of detection and so on (Sheppard, 2008;Alshannaq and Yae-Hiuk, 2017;Shanakhat et al, 2018). Trucksess and Zhang (2016) argued that all practically useful analytical methods should meet the basic Fluorescence polarization immunoassay 30 ng/ml Maragos, 2009 Biosensors 0.05 ml 0.005 µg/l Gurban et al, 2017;Man et al, 2017 Indirect methods Spectroscopy 4 µg/kg Wacoo et al, 2014 Emerging technologies Hyperspectral imaging 10 µg/kg Wang et al, 2014 Electronic nose 5 µ/kg Ottoboni et al, 2018 Aptamer-based biosensors ECL 0.1 pg/ml Shim et al, 2014;Castillo et al, 2015;Guo et al, 2016;Jia et al, 2019;Kordasht et al, 2019; guidelines of reproducibility in different laboratory settings. Based on these premises, protocols that are used in different laboratories from sampling to analysis were compiled, and systems relying on certified material samples (CRMs) are also closely related to this.…”

Recent progress in mycotoxins detection based on surface‐enhanced Raman spectroscopy

“…Comparing with traditional recognition reagents such as antibody, the major advantages of aptamer include low cost, wide range of targets, ease of preparation, and modification. So far, aptamer-based SERS detection of AFB 1 and OTA was the mostly reported, probably benefiting from the development of reliable aptamers for these two mycotoxins (Jia, Zhou, Liu, Li, & Yu, 2019;. To date, many aptasensors based on the functionalized magnetic beads have been widely established for highly sensitive detection of mycotoxins.…”

A Review of the Use of Carbon Nanotubes and Graphene-Based Sensors for the Detection of Aflatoxin M1 Compounds in Milk

“…Out of the applications related to electrochemical sensing, the detection of ions present in food is critical, as a slight aberration in the concentration of the chemical from its optimized value can increase the toxicity of the food product. Among them, aflatoxin M1 (ATM1) is one of the chemical compounds from a group and species of mycotoxins and aspergillus, respectively, that is found in milk and other food products [ 79 , 80 , 81 ]. While this chemical is primarily present in animal milk, the increase in its concentration can lead to adverse effects on the central nervous system, liver, kidney.…”