A novel adsorbent for protein chromatography: Supermacroporous monolithic cryogel embedded with Cu2+-attached sporopollenin particles

Erzengin, Mahmut; Ünlü, Nuri; Odabaşı, Mehmet

doi:10.1016/j.chroma.2010.11.074

Cited by 78 publications

(27 citation statements)

References 31 publications

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“…Macroporosity (') of the cryogel monolith was determined by measuring the free water content and volume of the monolith, of a given sample [45,46].…”

Section: Physical Properties Of the Cation-exchange Cryogelmentioning

confidence: 99%

Adsorptive behavior of α-lactalbumin on cation-exchange supermacroporous monolithic column

Machado

Minim

Fontan

et al. 2015

Fluid Phase Equilibria

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“…Macroporosity (') of the cryogel monolith was determined by measuring the free water content and volume of the monolith, of a given sample [45,46].…”

Section: Physical Properties Of the Cation-exchange Cryogelmentioning

confidence: 99%

Adsorptive behavior of α-lactalbumin on cation-exchange supermacroporous monolithic column

Machado

Minim

Fontan

et al. 2015

Fluid Phase Equilibria

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“…Erzengin et al [200] synthesised cryogels with poly(2-hydroxyethyl methacrylate) crosslinked by N,N-methylene-bis-acrylamide and embedded with Cu 2+ -attached sporopollenin particles.…”

Section: Diffusion Of Small Molecules Polymers Proteins and Cells Wmentioning

confidence: 99%

Cryogels: Morphological, structural and adsorption characterisation

Gun'ko

Savina

Mikhalovsky

2013

Advances in Colloid and Interface Science

281

205

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*Graphical Abstract (for review)Cryotripic gelation technique allows the synthesis of solid and soft macroporous gels Textural characterisation of cryogel in native superhydrated state with noninvasive methods Adsorption and diffusion characteristics of macroporous cryogels with respect to macromolecules and cells Theoretical modelling of adsorption from aqueous media *Highlights (for review) Please find the revised manuscript entitled "Cryogels: morphological, structural and adsorption characterisation" by Vladimir M. Gun'ko, Irina N. Savina, Sergey V. Mikhalovsky to re-consider for publication in the "Advances in Colloid and Interface Science"The paper was completely edited and changed according to reviewers comments.Sincerely yours, Prof. V. M. Gun'koCover Letter ABSTRACTExperimental results on polymer, protein, and composite cryogels and data treatment methods used for morphological, textural, structural, adsorption and diffusion characterisation of the materials are analysed and compared. Treatment of microscopic images with specific software gives quantitative structural information on both native cryogels and freeze-dried materials that is useful to analyse the drying effects on their structure. A combination of cryoporometry, relaxometry, thermoporometry, small angle X-ray scattering (SAXS), equilibrium and kinetic adsorption of low and high-molecular weight compounds, diffusion breakthrough of macromolecules within macroporous cryogel membranes, studying interactions of cells with cryogels provides a consistent and comprehensive picture of textural, structural and adsorption properties of a variety of cryogels. This analysis allows us to establish certain regularities in the cryogel properties related to narrow (diameter 0.4 < d < 2 nm), middle (2 < d < 50 nm) and broad (50 < d < 100 nm) nanopores, micropores (100 nm < d < 100 m) and macropores (d > 100 m) with boundary sizes within modified life science pore classification. Particular attention is paid to water bound in cryogels in native superhydrated or freeze-dried states. At least, five states of water -free unbound, weakly bound (changes in the Gibbs free energy G < 0.5-0.8 kJ/mol) and strongly bound (G > 0.8 kJ/mol), and weakly associated (chemical shift of the proton resonance  H = 1-2 ppm) and strongly associated ( H = 3-6 ppm) waters can be distinguished in hydrated cryogels using 1 H NMR, DSC, TSDC, TG and other methods. Different software for image treatment or developed to analyse the data obtained with the adsorption, diffusion, SAXS, cryoporometry and thermoporometry methods and based on regularisation algorithms is analysed and used for the quantitative morphological, structural and adsorption characterisation of individual and composite cryogels, including polymers filled with solid nano-or microparticles.

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“…The porosity (ϕ) value was estimated as the reported [17,19]. Briefly, a piece of cryogel sample was saturated with deionized water, and then immersed in water to yield volume V 1 .…”

Section: The Porosity Test Of the Cryogelsmentioning

confidence: 99%

“…To solve this problem, Savina et al [15] and Yao et al [16] prepared ion-exchange supermacroporous cryogels by in situ graft-polymerization, which could produce more binding sites for biomolecules on the graft-chains. Other groups prepared Fe 3 O 4 [17] or SiO 2 [18] nanoparticles, polymer beads [12] and bioparticles (Cu 2+ -attached sporopollenin particles) [19] embedded cryogels, respectively, which result the increase of surface areas within cryogels.…”

Section: Introductionmentioning

confidence: 99%

Monolithic cryogels made of agarose–chitosan composite and loaded with agarose beads for purification of immunoglobulin G

Sun

Tang

et al. 2012

International Journal of Biological Macromolecules

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A novel adsorbent for protein chromatography: Supermacroporous monolithic cryogel embedded with Cu2+-attached sporopollenin particles

Cited by 78 publications

References 31 publications

Adsorptive behavior of α-lactalbumin on cation-exchange supermacroporous monolithic column

Adsorptive behavior of α-lactalbumin on cation-exchange supermacroporous monolithic column

Cryogels: Morphological, structural and adsorption characterisation

Monolithic cryogels made of agarose–chitosan composite and loaded with agarose beads for purification of immunoglobulin G

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