A note on modification of pancreatic juice and bile collection method in sheep

Abstract

The aim of the study was to modify and evaluate the method of management of pancreatic juice and bile secretions in sheep in chronic experiment. Experiment was performed on 12 adult male Corriedale sheep of 54 ± 2.4 kg body weight, fi tted with two biomedical silicone catheters in the common bile duct according to the method described by Kato and Young (Kato et al., 1999) and our modifi cation duodenal cannula. Duodenal cannula was equipped with silicone valve in the basement part to prevent secretions backfl ow.Except the collection time, animals were freely moving in the individual pens and all secretions were returned to duodenum by means of constructed device. During all experimental treatments secretion volume of pancreatic juice was from 11.7 to 34.8 ml·h -1 . Modifi cation of Kato and Young method allows extending the period of animals use even up to more than 6 months. Moreover, it positively impacts animals welfare by reducing time spending in the metabolic cages, and saves labour expenses.