A not-stop-flow online normal-/reversed-phase two-dimensional liquid chromatography–quadrupole time-of-flight mass spectrometry method for comprehensive lipid profiling of human plasma from atherosclerosis patients

Li, Min; Tong, Xunliang; Lv, Pu; Feng, Baosheng; Yang, Li; Wu, Zheng; Cui, Xiaoxu; Bai, Yu; Huang, Yining; Liu, Huwei

doi:10.1016/j.chroma.2014.10.094

Cited by 47 publications

(29 citation statements)

References 35 publications

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“…5), which cannot be distinguished by MS method alone. However, these two classes of sphingolipids have been reported that they may have different effects in metabolism [13]. In this work, these two classes of isomers were successfully separated by our novel method.…”

Section: Separation Of Sphingolipidsmentioning

confidence: 68%

“…The details of this identification method were described in our previous works [13,[21][22][23][24]. In the end, 12 sphingolipids were identified by high-accuracy mass values and retention time, including one SPAH, two Cers, five GluCs, and four LacCs.…”

Section: Multivariate Statistical Analysismentioning

confidence: 99%

“…In previous work, we set up an NP/RP 2D LC-QToF MS method for lipid profiling of plasma of atherosclerosis patients and healthy volunteers [13]. In that work, 17 classes in 4 categories of lipids were analyzed at the same time, including fatty acyls, glycerolipids, glycerophospholipids and sphingolipids.…”

Section: Introductionmentioning

confidence: 99%

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Sphingolipids Profiling of Plasma in Patients with Diabetes Mellitus Associated with Atherosclerosis by a Novel Normal-Phase UPLC-QToF MS Method

Yang

Huang

et al. 2017

J. Anal. Test.

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A novel normal-phase UPLC-QToF MS method was developed for sphingolipids analysis, which was then applied to separate and identify seven classes of sphingolipids in plasma. The time-consuming of this novel method was much shorter than previous 2D LC-MS method, while the sensitivity, repeatability and resolution for sphingolipids analysis were better or comparable. Besides, the differences of lipids in human plasma among diabetes mellitus patients, diabetes mellitus associated with atherosclerosis patients and control subjects were also compared. Finally, 12 sphingolipids species were identified as the amounts of which in diabetes mellitus associated with atherosclerosis patients were significantly higher than them in diabetes mellitus patients (VIP [1.0, p value \0.05, fold change [1.5). As the isomers of GalCs and GluCs were successfully separated, it was found that five GluCs, rather than GalCs, were included in these 12 potential biomarkers. These results suggest the need to separate GalCs from GluCs, and also indicated that there may be some extra activation pathways of diabetes mellitus associated with atherosclerosis, different from the atherosclerosis pathway and the diabetes mellitus pathway, causing cardiovascular disease.

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Section: Separation Of Sphingolipidsmentioning

confidence: 68%

Section: Multivariate Statistical Analysismentioning

confidence: 99%

See 1 more Smart Citation

Sphingolipids Profiling of Plasma in Patients with Diabetes Mellitus Associated with Atherosclerosis by a Novel Normal-Phase UPLC-QToF MS Method

Yang

Huang

et al. 2017

J. Anal. Test.

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“…However, the coverage of lipid classes or lipid species is restricted in these reports. On the contrary, normal-phase/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (NP/RP 2D LC-QToF/MS)-based method could realize the analysis of more than 500 lipids from 17 lipid classes due to its high resolving power and sensitivity, which has been utilized for lipid analysis from atherosclerosis, breast cancer, and lacunar infarction patients in our previous studies [14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Plasma Lipidomic Analysis to Identify Novel Biomarkers for Hepatocellular Carcinoma

Yang

Bai²,

Han

et al. 2017

J. Anal. Test.

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Hepatocellular carcinoma (HCC) accounts for the major primary liver cancer, and its morbidity and mortality rates are very high. Identification of novel biomarkers for the early diagnosis and treatment of this disease is of crucial importance. In this study, a continuous-flow normal-phase/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (NP/RP 2D LC-QToF/MS) method was used to detect lipid species in human plasma from HCC patients and healthy controls. As a result, the two groups were clearly separated by principal component analysis. Besides, 23 lipid species with corrected p value \0.05 and fold change [1.5 were proposed as potential lipid biomarkers for HCC. Among them, 9 lipid species, including galactosylceramide (36:5), free fatty acid (20:4), phosphatidylethanolamine (40:6), phosphatidylethanolamine (38:6), diacylglycerol (40:5), diacylglycerol (44:2), lactosylceramide (40:3), phosphatidylcholine (40:6), and free fatty acid (22:5), exhibited excellent ability to distinguish HCC patients from healthy controls with the area under the receiver-operating characteristic curve [0.900, showing promising clinical value. Taken together, these results indicate the great potential of NP/RP 2D LC-QToF/MS method in disease diagnosis, which might be helpful in clinical practice.

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“…Currently, dominated approaches of separation and identification of lipid molecules are mass spectrometry (MS) [5,6] and liquid chromatography-mass spectrometry (LC-MS) [7,8]. Chiral analysis has been attempted using mass spectrometry by generating chiral environment, performing tandem MS experiment or relying on kinetic method [9].…”

Section: Introductionmentioning

confidence: 99%

Supercritical Fluid Chromatography and Its Application in Lipid Isomer Separation

2017

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Supercritical fluid chromatography (SFC), which uses supercritical fluid with higher diffusivity and lower viscosity than liquid as its mobile phase, can provide fast, green, high-throughput and good performance methods for the analysis of complicated samples. It is an advantageous alternative for the separation of polar and nonpolar compounds, and even chiral isomers. As an important constituent of living organisms, lipids and the related lipidomics researches have attracted dramatic attention during the past years. The separation and identification of lipids and lipid isomers are of great significance and remain challenging. In this mini-review, the principle of SFC and its applications in lipid isomer separation are summarized.

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A not-stop-flow online normal-/reversed-phase two-dimensional liquid chromatography–quadrupole time-of-flight mass spectrometry method for comprehensive lipid profiling of human plasma from atherosclerosis patients

Cited by 47 publications

References 35 publications

Sphingolipids Profiling of Plasma in Patients with Diabetes Mellitus Associated with Atherosclerosis by a Novel Normal-Phase UPLC-QToF MS Method

Sphingolipids Profiling of Plasma in Patients with Diabetes Mellitus Associated with Atherosclerosis by a Novel Normal-Phase UPLC-QToF MS Method

Plasma Lipidomic Analysis to Identify Novel Biomarkers for Hepatocellular Carcinoma

Supercritical Fluid Chromatography and Its Application in Lipid Isomer Separation

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