A nortriterpenoid, triterpenoids and ecdysteroids from Pfaffia glomerata

Shiobara, Yoshinori; Inoue, Shun-suke; Kato, Keiko; Nishiguchi, Yukari; Oishi, Yukako; Nishimoto, Nobushige; Oliveira, Fernando de; Akisue, Gokithi; Akisue, Maria Kubota; Hashimoto, Goro

doi:10.1016/0031-9422(93)85172-n

Cited by 81 publications

(54 citation statements)

References 7 publications

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“…Segundo Mantell et al (1994), as taxas de multiplicação, em geral, de 5-10x, variam de acordo com o genótipo e composição do meio de cultura. A produção de segmentos nodais é um parâmetro importante na propagação in vitro de P. glomerata, pois reflete a produção de novas plantas a cada subcultivo e, de acordo com Zaerr & Mapes (1985), BAP é a citocinina mais eficaz para a multiplicação de partes aéreas da maioria das espécies.…”

Section: Resultado E Discussãounclassified

“…Além disso, pesquisas conduzidas com extratos fracionados de raízes de P. glomerata demonstraram a atividade antihiperglicemiante da planta (Sanches et al, 2001). Estes efeitos farmacológicos de P. glomerata são atribuídos à presença de vários compostos químicos isolados a partir das raízes das plantas, destacandose as saponinas triterpênicas e os fitoecdisteróides (Shiobara et al, 1993).…”

Section: Introductionunclassified

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Benzilaminopurina (BAP) e thidiazuron (TDZ) na propagação in vitro de Pfaffia glomerata (Spreng.) Pedersen

Flores

Nicoloso

Maldaner

et al. 2009

Rev. bras. plantas med.

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RESUMO:Pfaffia glomerata (Spreng.) Pedersen, conhecida como ginseng brasileiro, é muito utilizada pela medicina popular devido suas propriedades fitoterápicas. Este trabalho teve como objetivo avaliar o efeito de concentrações de BAP e de TDZ na propagação in vitro de dois acessos (BRA e JB-UFSM) desta espécie. Segmentos nodais, provenientes de plantas assépticas, foram cultivados em meio Murashige e Skoog (MS) suplementado com 0, 1 e 5 M de BAP ou TDZ. Após 30 dias, as plantas foram transferidas para meio MS não suplementado com citocinina. Observou-se que a organogênese in vitro de P. glomerata é genótipo-dependente. O acesso BRA mostrou um maior potencial para a propagação in vitro em relação ao JB-UFSM. O cultivo dos segmentos nodais do acesso BRA em meio com 1 M de TDZ, seguido pelo subcultivo dos brotos para meio isento de citocininas mostrou ser um método viável para a propagação in vitro devido à alta taxa de multiplicação e o bom desenvolvimento das plantas. No acesso JB-UFSM, o cultivo dos segmentos nodais em meio não acrescido de citocinina mostrou ser o método mais adequado para a produção de um grande número de plantas com alta qualidade.Palavras chave: Amaranthaceae, ginseng brasileiro, cultivo in vitro, citocininas ABSTRACT: Benzylaminopurine (BAP) and thidiazuron (TDZ) on in vitro propagation ofPfaffia glomerata (Spreng.) Pedersen. The plant Pfaffia glomerata (Spreng.) Pedersen, known as Brazilian ginseng, is extensively used in folk medicine due to its phytotherapic characteristics. This work aimed to evaluate the effect of BAP and TDZ concentrations on the in vitro propagation of two sources (BRA and JB-UFSM) of this species. Nodal segments originated from aseptically grown plants were cultured on Murashige and Skoog (MS) medium supplemented with 0, 1 and 5 M BAP or TDZ. After 30 days, the plants were transferred to MS medium without cytokinin. The in vitro organogenesis of P. glomerata is genotype-dependent. The source BRA had greater potential for in vitro propagation than JB-UFSM. The culture of BRA nodal segments on medium with 1 M TDZ, followed by subcultivation of sprouts on cytokinin-free medium, showed to be a viable method for in vitro propagation due to high multiplication rate and good plant development. In the source JB-UFSM, the culture of nodal segments on medium without cytokinin was the best method to obtain a large number of high-quality plants.

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Section: Resultado E Discussãounclassified

Section: Introductionunclassified

Benzilaminopurina (BAP) e thidiazuron (TDZ) na propagação in vitro de Pfaffia glomerata (Spreng.) Pedersen

Flores

Nicoloso

Maldaner

et al. 2009

Rev. bras. plantas med.

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“…Shiobara et al (1993), identified glomeric acid (triterpenoid) and pfameric acid (nortriterpenoid), β-ecdysone, rubrosterone, oleanolic acid and β-glucopyranosyl oleanolate in Pfaffia glomerata (Spreng.) Pederson.…”

Section: Introductionmentioning

confidence: 99%

Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

Serra¹,

Felipe²,

Cortez³

2012

Rev. bras. farmacogn.

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Pfaffi a glomerata (Spreng.) Pedersen, Amaranthaceae, is widely distributed in Brazil. Roots are considered as the world's greatest supplier and β-ecdysone is the most important compound extracted from roots of Pfaffi a glomerata. So, the aim this study was analyze the presence of β-ecdysone in the infl orescences and stems and compared with the content from roots of Pfaffi a glomerata and determine the best extractive method of β-ecdysone this plant. The crude extracts were obtained by Soxhlet method, refl ux, maceration, percolation and turbolyse. Compound extracts were quantifi ed by High Performance Liquid Chromatography (HPLC). The analysis were carried out a Phenomenex Column C18, 5 µm, 250x4,6mm, maintened at 30 o C, gradient system using as mobile phase a mixture of methanol and water, fl ow rate 1,0 mL and detection at 245 nm. Results showed Soxhlet method with ethanol:water (90:10 v/v) presented the higher concentration of β-ecdysone in P. glomerata and infl orescences showed higher amount of this active substance (3,06%), compared with stems (2,37%) and roots (1,63%), showing that the infl orescences and plant stems may also be used as a rich source of β-ecdysone.

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“…4) On the other hand, ecdysterone has been characterized as the principal constituent from the roots. 5,6) However, chemical and pharmacological studies on the roots of P. glomerata have not been investigated sufficiently yet. During the course of characterization studies on the bioactive constituents of Brazilian natural medicines, 1,[7][8][9][10] the ethyl acetate (EtOAc) and 1-butanol (1-BuOH) soluble fractions of the roots of P. glomerata were found to show inhibitory effects on melanogenesis in theophylline-stimulated B16 melanoma 4A5 cells.…”

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Brazilian Natural Medicines. IV. New Noroleanane-Type Triterpene and Ecdysterone-Type Sterol Glycosides and Melanogenesis Inhibitors from the Roots of Pfaffia glomerata

et al. 2010

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The Amaranthaceae plant, Pfaffia (P.) glomerata, which is so-called as Brazil ginseng in Japan, is widely cultivated in South American countries such as Brazil, Ecuador, and Panama. The roots of this plant are used as a Brazilian folk medicine for a tonic and treatment of diabetes. The extract from the roots of P. glomerata has been reported to possess gastroprotective, 2) anti-oxidant, 3) and anti-inflammatory effects.4) On the other hand, ecdysterone has been characterized as the principal constituent from the roots.5,6) However, chemical and pharmacological studies on the roots of P. glomerata have not been investigated sufficiently yet. During the course of characterization studies on the bioactive constituents of Brazilian natural medicines, 1,7-10) the ethyl acetate (EtOAc) and 1-butanol (1-BuOH) soluble fractions of the roots of P. glomerata were found to show inhibitory effects on melanogenesis in theophylline-stimulated B16 melanoma 4A5 cells. From the EtOAc and 1-BuOH soluble fractions, we have isolated a new noroleanane-type triterpene, pfaffianol A (1), two new noroleanane-type triterpene glycosides, pfaffiaglycosides A (2) and B (3), and three ecdysterone-type sterol glycosides, pfaffiaglycosides C (4), D (5), and E (6), with eight known constituents. Furthermore, we examined the inhibitory effects of the fractions and principal constituents on melanogenesis in theophylline-stimulated B16 melanoma 4A5 cells. In this paper, we describe the isolation and structure elucidation of the new constituents The ethyl acetate and 1-butanol soluble fractions of the roots of Pfaffia glomerata were found to show inhibitory effects on melanogenesis in theophylline-stimulated B16 melanoma 4A5 cells. From the ethyl acetate and 1-butanol soluble fractions, we isolated a new noroleanane-type triterpene, pfaffianol A, its glycosides, pfaffiaglycosides A and B, and ecdysterone-type sterol glycosides, pfaffiaglycosides C, D, and E, together with eight known constituents. The structures of new constituents were determined on the basis of physicochemical and chemical evidence. Among them, pfaffianol A (IC 50 ‫؍‬ ‫44؍‬ m mM) and pfaffoside C (IC 50 ‫؍‬ ‫29؍‬ m mM) substantially inhibited melanogenesis without cytotoxic effects. The inhibitory effects were stronger than that of reference compound, arbutin (IC 50 ‫؍‬ ‫471؍‬ m mM).

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A nortriterpenoid, triterpenoids and ecdysteroids from Pfaffia glomerata

Cited by 81 publications

References 7 publications

Benzilaminopurina (BAP) e thidiazuron (TDZ) na propagação in vitro de Pfaffia glomerata (Spreng.) Pedersen

Benzilaminopurina (BAP) e thidiazuron (TDZ) na propagação in vitro de Pfaffia glomerata (Spreng.) Pedersen

Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC

Brazilian Natural Medicines. IV. New Noroleanane-Type Triterpene and Ecdysterone-Type Sterol Glycosides and Melanogenesis Inhibitors from the Roots of Pfaffia glomerata

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A nortriterpenoid, triterpenoids and ecdysteroids from Pfaffia glomerata

Cited by 81 publications

References 7 publications

Benzilaminopurina (BAP) e thidiazuron (TDZ) na propagação in vitro de Pfaffia glomerata (Spreng.) Pedersen

Benzilaminopurina (BAP) e thidiazuron (TDZ) na propagação in vitro de Pfaffia glomerata (Spreng.) Pedersen

Quantification of &#946;-ecdysone in differents parts of Pfaffia glomerata by HPLC

Brazilian Natural Medicines. IV. New Noroleanane-Type Triterpene and Ecdysterone-Type Sterol Glycosides and Melanogenesis Inhibitors from the Roots of Pfaffia glomerata

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Quantification of β-ecdysone in differents parts of Pfaffia glomerata by HPLC