A nonoccluded virus of Junonia coenia (Nymphalidae: Lepidoptera)

Rivers, C. F.; Longworth, J. F.

doi:10.1016/0022-2011(72)90173-5

Cited by 44 publications

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“…Optimizing of the transfection protocol and using a quantitative b-gal assay (ONPG) showed that the maximum amount of b-gal was expressed on the third day post-transfection when 10 6 cells were transfected with 10 to 20 lg of the plasmid. The X-gal staining in the positive cells was noticeably more intense in the nucleus than in the cytoplasm, presumably as a result of targeting the hybrid VP-b-gal proteins into nuclei by the VP portion, since the JcDNV capsid proteins were shown to have nuclear localization [26]. Analysis of the expression products by western blot, using anti-b-gal antibodies, revealed four proteins, three of which corresponded to b-gal fused to the N-terminal fragments of VP2, VP3 and VP4, and a polypeptide with a lower molecular weight which could be either a product of partial degradation of the b-gal fusion proteins or a product of initiation from an ATG codon located downstream of the one assigned for initiation of VP4.…”

Section: Jcdnv and Gmdnv (Genus Densovirus) Expression Systemsmentioning

confidence: 98%

Densovirinae as Gene Transfer Vehicles

Afanasiev

Carlson²

2000

Contributions to Microbiology

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Densoviruses present an attractive opportunity to develop expression vectors for insects. They exhibit several features that are beneficial for such vectors. The genomes of densoviruses are among the smallest of animal DNA viruses, and, therefore are easier to use for cloning and transfection procedures. The fact that cloned densovirus genomes are infectious greatly simplifies their applications. It seems that both densovirus promoters have high constitutive activity and they can be further transactivated with NS. The major nonstructural protein, NS1, can retain its functions even if fused to a foreign protein. If the recombinant genome carrying a gene of interest is supplied with the missing virus functions, it can be packaged into transducing particles. The packaging capacity of the densovirus particles can accommodate a foreign gene encoding a polypeptide of up to 150 kDa. Finally, the functions missing from the recombinant densovirus genome that are necessary for the production of transducing particles can be supplied in several different ways: they can be expressed from a helper plasmid, in cells transformed with the virus genes, or from an RNA virus. The last two methods produce transducing particles which are free of wild type virus. Densovirus transducing particles can be used for the delivery and expression of a gene of interest in insect cell culture or living insects. Transducing particles expressing reporter genes can be valuable tools in the study of viral pathogenesis and perhaps in the genetic manipulation of insects.

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Section: Jcdnv and Gmdnv (Genus Densovirus) Expression Systemsmentioning

confidence: 98%

Densovirinae as Gene Transfer Vehicles

Afanasiev

Carlson²

2000

Contributions to Microbiology

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“…is a butterfly in the Nymphalidae family. A nonoccluded, small virus was isolated from caterpillar cadavers (1) and subsequently characterized as a densovirus (2). This Junonia coenia densovirus (JcDNV) was kindly provided to us by T. W. Tinsley (NERC Institute of Virology, Oxford).…”

Section: Genome Announcementmentioning

confidence: 99%

Junonia coenia Densovirus (JcDNV) Genome Structure

Pham¹,

Huynh²,

Jousset

et al. 2013

Genome Announc

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The sequence of Junonia coenia densovirus was the first densovirus genome sequence published, but the first published sequence contained incomplete inverted terminal repeats and ambiguous nucleotides or indels leading to an incorrect map of the open reading frames. Our sequencing of clones of the complete genome demonstrated that this virus is closely related to other viruses in the Densovirus genus.

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“…The GmDNV, CeDNV, and AdDNV have a host range apparently restricted to their original hosts, since attempts to multiply them in alternative hosts repeatedly failed (Giran, 1966;Jousset et a1., 1986;Fediere et a1., 19901. In contrast, other DNVs, also isolated from lepidoptera, have a broader host range. Thus, the JcDNV can replicate in Aglais urticae, Lymantria dispar, B. mori, Mamestra brassicae, M. oleracea, S. littoralis, and S. exigua (Rivers and Longworth, 1972;Diallo, 1978). Similarly, the EaDNV is infectious for Pseudaletia unipuncta and Heliothis zea (Sutter, 1973), and the host range of MIDNV extends to S. littoralis, Pectinophora gossypiella, Sesamia cretica, Chilo agamemnon, and Ostrinia nubilalis (Fediere, 1996).…”

Section: Ultrastructure Of Infected Cellsmentioning

confidence: 99%

The Insect Viruses

1998

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viii PREFACE an environmental perspective. Viral diseases in beneficial insects can lead to ecological problems, while diseases of insect pests are often welcomed as a means of reducing economic and medical problems. Furthermore, like the baculoviruses, some of the viruses described in this volume are likely to become valuable tools in the escalating human efforts to control and manipulate the ecosystem.The large DNA viruses are described first, beginning with the entomopoxviruses and iridoviruses, followed by those that rely more heavily on insect vectors for their propagation, and then the small DNA densoviruses. These are followed by the RNA viruses, beginning with an account of the three-dimensional structures of small RNA viruses, followed by chapters describing each of the major families of insect RNA viruses, and ending with a review of the development of arbovirus expression systems and how they may be employed in the future.

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A nonoccluded virus of Junonia coenia (Nymphalidae: Lepidoptera)

Cited by 44 publications

References 0 publications

Densovirinae as Gene Transfer Vehicles

Densovirinae as Gene Transfer Vehicles

Junonia coenia Densovirus (JcDNV) Genome Structure

The Insect Viruses

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