Junonia coenia Densovirus (JcDNV) Genome Structure

Abstract

The sequence of Junonia coenia densovirus was the first densovirus genome sequence published, but the first published sequence contained incomplete inverted terminal repeats and ambiguous nucleotides or indels leading to an incorrect map of the open reading frames. Our sequencing of clones of the complete genome demonstrated that this virus is closely related to other viruses in the Densovirus genus.

Transcytosis of Junonia coenia densovirus VP4 across the gut epithelium of Spodoptera frugiperda (Lepidoptera: Noctuidae)

“…Junonia coenia densovirus (JcDNV) ( Ambidensovirus ; Parvoviridae ) is a small, single‐stranded DNA virus that is lepidopteran‐specific (Bergoin & Tijssen, ; Pham et al ., ) and highly pathogenic to a few species, including Spodoptera frugiperda (J.E. Smith) (Rivers & Longworth, ; Bergoin & Tijssen, ; Mutuel et al ., ).…”

A Novel Ambisense Densovirus, Acheta domesticus Mini Ambidensovirus, from Crickets

“…Surprisingly, the genome structure and gene organization of this virus strongly resembled those of ambisense densoviruses from the Densovirus genus ( 7 – 11 ), but the genome sequence was only 4,945 nucleotides (nt) long, instead of about 6,035 nt, and lacked nucleotide sequence identity (best E value of 0.017, with a query coverage of 1%). Protein sequence identities were for the major nonstructural protein 1 (NS1) closest to Iteravirus members and, oddly, for the structural proteins (VP) closest to Densovirus members (both at best 25% identity for 25% coverage [or higher for shorter coverage]).…”

“…Two genera of insect parvoviruses, named densoviruses ( 6 ), are particularly relevant for this new densovirus. The Densovirus genus contains ambisense densoviruses from Lepidoptera , with genomes of 6 kb, Y-shaped inverted terminal repeats (ITRs) of about 0.55 kb, and sequence identities of about 85% ( 7 – 11 ). The Iteravirus genus contains monosense densoviruses, also from Lepidoptera , with 5-kb genomes, J-shaped 0.25-kb inverted terminal repeats (ITRs), and about 75% sequence identities ( 12 – 15 ).…”

Comparative Genomic Analysis ofAcheta domesticusDensovirus Isolates from Different Outbreaks in Europe, North America, and Japan

“…For all isolates, DNA samples were isolated as previously described (5). Primers (5) were designed on the sequence obtained previously for the 1977 AdDNV isolate from Europe (GenBank accession number HQ827781), so that overlapping amplicons could be obtained, whereas termini were cloned as described previously (7, 8). …”