A non-invasive identification of hormone metabolites, gonadal event and reproductive status of captive female tigers

Abstract

Putranto HD (2011) A non-invasive identification of hormone metabolites, gonadal event and reproductive status of captive female . As a non-invasive method, fecal sample provides some advantage for animal and collector. The purpose of the present study were to monitor the reproductive status of female Siberian tigers (Panthera tigris altaica) by assessing changes in fecal during natural ovarian activity and pregnancy and to identify whether progesterone (P4) exists and what kinds of P4 metabolites excreted into the feces. Two female tigers were fed a diet consisting of meat. Drinking water was available ad libitum. Feces were collected ones to twice a week. The fecal contents of P4 and estradiol-17β (E2) were determined by EIA and P4 metabolites were separated by a modified HPLC. The EIA results shown that during its natural ovarian activity the E2 contents showed cyclic changes at the average of 27.0 d interval, however, no distinct cycles were shown in fecal P4 contents of non-pregnant tiger. In contrary, the fecal P4 contents in pregnant tiger increased remarkably after copulation approximately 2-to 6-fold higher than the mean value. The HPLC results indicated that two peaks were primarily detected fraction 63-64 min (identified metabolites) and fraction 85 min (not identified metabolite) in feces of pregnant tiger. However, P4 detected only small amount in feces. It is possible to assess non-invasively gonadal events such as luteal or follicular activity or ovulation of Siberian tigers by endocrine monitoring based on fecal P4 and E2 to understand reproductive status.

Estradiol-17β hormone concentration and follicles number in exotic Burgo chicken supplemented by Sauropus androgynus leaves extract

“…When the primordial follicle grows until the stage of de Graff follicle, those hormones are mutually interacted. Previous studies also reported that sexual steroid hormones such as P4, E2, estrone (E1), immunoreactive pregnanediol glucoronide (PdG), estradiol glucoronide (E2G) of female can be used widely to monitor natural ovarian activity such as functional luteal activity and follicular maturation and to confirm pregnancy (Adachi et al 2011;Kusuda et al 2006Kusuda et al , 2007aPutranto 2011c;Putranto et al 2007a, b, c).…”

Effect of katuk (Sauropus androgynus) powder supplementation on the levels of progesterone (P4) and estradiol-17β (E2) hormones in kacang goat (Capra aegragus)

“…The assessment of levels of reproductive hormone was done in some of wild animals such as Sumatera tigers and Siberia tigers (Putranto et al 2007a(Putranto et al , 2007b, Sumatera Orang Utan, Sambar deer (Putranto et al 2011) and Burgo chicken (Putranto et al 2012). In this study, the measurement of levels of reproductive hormone in the kacang goats treated by the katuk powder supplementation in the animal feed was the first research paper published in scientific journal.…”

“…In this case, P4 antiserum will react mainly with P4 (100%), 5α-pregnanedione (62.2%), pregnenolone (6.3%), 11-deoxycorticosterone (3.9%), 17α-hydroxyprogesterone (2.3%), and 11α-hydroxyprogesterone (1.2%) (Putranto et al 2011). Meanwhile, E2 antiserum will react mainly with E2 (100%), estrone-2-sulfate (8%), 16-epiestriol (5.3%), estrone (3.2%), and estriol (1.8% ) (Putranto et al 2012).…”

Effects of PMSG and HCG on estradiol and progesterone levels from common palm civets (Paradoxurus hermaphroditus)

“…Tất cả các mức độ hormone được biểu thị bằng microgam trên gam phân khô (μg/g df). Hàm lượng E2 và P4 cực đại (đỉnh-peak) được xác định là những giá trị lớn hơn trung bình của tất cả các giá trị còn lại từ mỗi cá thể Cầy Vòi hương (Putranto, 2011). Các tham số thống kê được xử lí bằng phần mềm MS-Excel 2013.…”

“…Hình 1 cho thấy, hàm lượng E2 trong phân của cầy ở các nghiệm thức bắt đầu tăng lên ở ngày thứ 1 sau khi tiêm, tăng đạt đỉnh vào ngày thứ 2 và sau đó giảm dần từ ngày thứ 3. Thời gian chậm trễ (lag time) giữa E2 trong phân và trong huyết thanh ở các loài thuộc bộ ăn thịt (Carnivora) trung bình từ 5-12 giờ (Putranto, 2011). Như vậy, sự thay đổi hàm lượng E2 trong máu đã tăng lên trước đó, chứng tỏ có sự ảnh hưởng của PMSG và HCG đến hormone sinh dục của Cầy Vòi hương.…”