A NOMPC-Dependent Membrane-Microtubule Connector Is a Candidate for the Gating Spring in Fly Mechanoreceptors

Liang, Xin; Madrid, Johnson; Gärtner, Roland; Verbavatz, Jean‐Marc; Schiklenk, Christoph; Wilsch‐Bräuninger, Michaela; Bogdanova, Aliona; Stenger, Florian; Voigt, Axel; Howard, Jonathon

doi:10.1016/j.cub.2013.03.065

Cited by 82 publications

(112 citation statements)

References 35 publications

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“…TRP channels have been implicated in the mechanotransduction of adult Cho neurons. The TRPN channel NOMPC is important for hearing in adult Drosophila, as well as in larval locomotion and gentle touch behavior (18)(19)(20)(21)(36)(37)(38). Immunostaining revealed expression of NOMPC protein in the cilium of larval Cho neurons (Fig.…”

Section: Resultsmentioning

confidence: 98%

Sound response mediated by the TRP channels NOMPC, NANCHUNG, and INACTIVE in chordotonal organs of Drosophila larvae

Zhang

Yan

Jan

2013

Proc. Natl. Acad. Sci. U.S.A.

134

147

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Mechanical stimuli, including tactile and sound signals, convey a variety of information important for animals to navigate the environment and avoid predators. Recent studies have revealed that Drosophila larvae can sense harsh or gentle touch with dendritic arborization (da) neurons in the body wall and can detect vibration with chordotonal organs (Cho). Whether they can also detect and respond to vibration or sound from their predators remains an open question. Here we report that larvae respond to sound of wasps and yellow jackets, as well as to pure tones of frequencies that are represented in such natural sounds, with startle and burrowing behaviors. The larval response to sound/ vibration requires Cho neurons and, to a lesser extent, class IV da neurons. Our calcium imaging and electrophysiological experiments reveal that Cho neurons, but not class IV da neurons, are excited by natural sounds or pure tones, with tuning curves and intensity dependence appropriate for the behavioral responses. Furthermore, our study implicates the transient receptor potential (TRP) channels NOMPC, NANCHUNG, and INACTIVE, but not the dmPIEZO channel, in the mechanotransduction and/or signal amplification for the detection of sound by the larval Cho neurons. These findings indicate that larval Cho, like their counterparts in the adult fly, use some of the same mechanotransduction channels to detect sound waves and mediate the sensation akin to hearing in Drosophila larvae, allowing them to respond to the appearance of predators or other environmental cues at a distance with behaviors crucial for survival.

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Section: Resultsmentioning

confidence: 98%

Sound response mediated by the TRP channels NOMPC, NANCHUNG, and INACTIVE in chordotonal organs of Drosophila larvae

Zhang

Yan

Jan

2013

Proc. Natl. Acad. Sci. U.S.A.

134

147

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“…In the class IV subset of these neurons, the piezo channel, which is not a TRP channel, transduces stronger, noxious touch stimuli, while in the class III neurons nompC transduces soft touch. 29) NompC is also essential in the transduction of cuticular deformation by campaniform sensillae, 30) in auditory transduction by Johnston's organ in adult Drosophila 31,32) and in vibration detection by the lateral (pentascolopidial) chordotonal organs in Drosophila larvae. 33) NOMPC is also present in the distal tip of the cilium of leg chordotonal organs, where it is likely to also be involved in sensory transduction for the proprioceptive sense.…”

Section: Trpnmentioning

confidence: 99%

Insect TRP channels as targets for insecticides and repellents

Salgado

2017

J. Pestic. Sci.

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This review provides a brief overview of ion channels, then focuses on TRP channels, describing the properties and functions of the seven TRP channel classes found in insects. Finally, recent work showing that a heteromeric channel composed of Nanchung and Inactive vanilloid TRP (TRPV) channel subunits is the target of the selective feeding blockers pymetrozine and pyrifluquinazon is described. The possible utility of other TRP channels as targets of insecticides and repellents is also considered. © Pesticide Science Society of Japan

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“…[9][10][11][12] Furthermore, microtubules support channel activity 13 ; for example, the coupling of the transient receptor potential family mechanotransduction channel NompC to an array of microtubules conveys force to gate channel activation. [14][15][16] Dendrite microtubule architecture differs between neuron types 15,[17][18][19][20] in order to support the specific functional requirements of each type. We illustrate that here for the specialized dendrites of class IV nociceptive 21 and class I proprioceptive 22 Drosophila body wall neurons.…”

mentioning

confidence: 99%

Microtubule nucleation and organization in dendrites

2016

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A NOMPC-Dependent Membrane-Microtubule Connector Is a Candidate for the Gating Spring in Fly Mechanoreceptors

Abstract: Our results provide strong evidence that the ankyrin-repeat domains of NOMPC structurally contribute to the membrane-microtubule connecting filaments. These filaments, as the most compliant element in the distal tip, are therefore good candidates for the gating springs.

Cited by 82 publications

References 35 publications

Sound response mediated by the TRP channels NOMPC, NANCHUNG, and INACTIVE in chordotonal organs of Drosophila larvae

Sound response mediated by the TRP channels NOMPC, NANCHUNG, and INACTIVE in chordotonal organs of Drosophila larvae

Insect TRP channels as targets for insecticides and repellents

Microtubule nucleation and organization in dendrites

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