A no-carrier-added <sup>72</sup>Se/<sup>72</sup>As radionuclide generator based on distillation

Jennewein, M.; Schmidt, A.; Novgorodov, A.F.; Qaim, S.M.; Rösch, Frank

doi:10.1524/ract.92.4.245.35611

Cited by 35 publications

(26 citation statements)

References 8 publications

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“…Jennewein and Rö sch have developed efficient methods for isolating arsenic from irradiated germanium oxide targets to provide arsenic in a form that is useful for labeling sensitive biomolecules (7,8). They have also developed novel methods for linking arsenic to biomolecules.…”

Section: Discussionmentioning

confidence: 99%

“…Radiochemistry has now evolved, and several isolation procedures for arsenic isotopes have been reported. Most recently, Jennewein and Rö sch developed efficient methods for isolating pure radionuclides from irradiated GeO 2 targets on the basis of a solid phase extraction system (7,8). Moreover, Jennewein and Rö sch proposed chemistry for the effective labeling of biologically relevant molecules, as we have now exploited.…”

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confidence: 99%

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Vascular Imaging of Solid Tumors in Rats with a Radioactive Arsenic-Labeled Antibody that Binds Exposed Phosphatidylserine

Jennewein

Lewis²,

Zhao³

et al. 2008

Clinical Cancer Research

130

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Purpose: We recently reported that anionic phospholipids, principally phosphatidylserine, become exposed on the external surface of vascular endothelial cells in tumors, probably in response to oxidative stresses present in the tumor microenvironment. In the present study, we tested the hypothesis that a chimeric monoclonal antibody that binds phosphatidylserine could be labeled with radioactive arsenic isotopes and used for molecular imaging of solid tumors in rats. As (h -,T 1/2 1.6 days) using a novel procedure. The radionuclides of arsenic were selected because their long half-lives are consistent with the long biological half lives of antibodies in vivo and because their chemistry permits stable attachment to antibodies. The radiolabeled antibodies were tested for the ability to image subcutaneous Dunning prostate R3227-AT1tumors in rats. Results: Clear images of the tumors were obtained using planar g-scintigraphy and positron emission tomography. Biodistribution studies confirmed the specific localization of bavituximab to the tumors. The tumor-to-liver ratio 72 h after injection was 22 for bavituximab compared with 1.5 for an isotype-matched control chimeric antibody of irrelevant specificity. Immunohistochemical studies showed that the bavituximab was labeling the tumor vascular endothelium. Conclusions: These results show that radioarsenic-labeled bavituximab has potential as a new tool for imaging the vasculature of solid tumors.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Vascular Imaging of Solid Tumors in Rats with a Radioactive Arsenic-Labeled Antibody that Binds Exposed Phosphatidylserine

Jennewein

Lewis²,

Zhao³

et al. 2008

Clinical Cancer Research

130

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“…every second day, it is theoretically possible to elute about 75% of the maximum daughter activity (∼ 40% to elute every 24 h) [10]. Fig.…”

Section: Se/ 72 As Radionuclide Generatormentioning

confidence: 96%

“…In parallel, a 72 Se/ 72 As radionuclide generator has been studied [9][10][11][12][13][14][15]. Strategies towards a versatile radioarsenic labelling chemistry were developed to generate arsenic isotopes in chemical forms suitable for future application in labelling chemistry, radiopharmacy and, ultimately, for molecular imaging using Positron *Author for correspondence (E-mail: frank.roesch@uni-mainz.de).…”

Section: Introductionmentioning

confidence: 99%

“…Another process using addition of selenium carrier in the form of selenic acid uses the cyclic reduction of selenium to Se (0) and a separation of 72 As by filtration with subsequent oxidative dissolution of Se (0) using H 2 O 2 prior to each separation cycle [11]. As published recently, our group developed a no-carrier-added generator based on distillation [10,23]. Following the Ge( 3 He, 3n) 72 Se process, the irradiated Ge was dissolved in aqua regia and germanium was removed via distillation as GeCl 4 .…”

Section: Introductionmentioning

confidence: 99%

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A no-carrier-added⁷²Se/⁷²As radionuclide generator based on solid phase extraction

Jennewein¹,

Qaim²,

Kulkarni³

et al. 2005

Radiochimica Acta

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Summary.72 As-labelled radiopharmaceuticals could be a valuable resource for Positron Emission Tomography (PET). In particular, the long half-life of 72 As (T 1/2 = 26 h) facilitates the observation of long-term physiological or metabolic processes, such as the enrichment and distribution of antibodies in tumor tissue. This work describes the primary radiochemical separation of no-carrier-added (nca) 72 Se from cyclotron irradiated germanium targets and the development of a polystyrene type solid-phase extraction based 72 Se/ 72 As radionuclide generator, avoiding the addition of any selenium carrier. The irradiated germanium target is dissolved in HF conc and selenium is reduced with hydrazine dihydrochloride. The nca 72 Se (0) is adsorbed on a solid-phase extraction cartridge, representing the generator column. The 72 As is eluted using various aqueous solvents with 40%-60% yield and < 0.1% 72 Se content. To be able to study the radiopharmaceutical arsenic chemistry, subsequent chemical modification of the nca 72 As eluates to nca [72 As]AsI 3 provides a versatile radioarsenic labelling synthon.

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Labelling with positron emitters of pnicogens and chalcogens

Neumaier

2017

Labelled Comp Radiopharmac

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The increasing importance of the positron emission tomography (PET) in clinical diagnosis led to the development of a multitude of radiotracers labelled with positron‐emitting radionuclides of groups 15 (pnicogens) and 16 (chalcogens) of the periodic table of elements. The positron emitters of the endogenous occurring elements nitrogen, phosphorus, oxygen, and sulphur are characterized by very short half‐lives compared with the most commonly used PET radionuclides carbon‐11 or fluorine‐18. Therefore, the potential of their synthesis and possible applications in PET is challenging and limited. On the other hand, the nonstandard positron emitters arsenic‐72, arsenic‐74, and selenium‐73 have half‐lives in the range of hours to days and, thus, are of interest for PET studies of processes with long biological half‐lives, but novel methods have to be developed for their application, especially in the no‐carrier‐added state. This review summarizes recent research concerning the positron emitters of pnicogens and chalcogens for radiolabelling applications.

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A no-carrier-added ⁷²Se/⁷²As radionuclide generator based on distillation

Abstract: Arsenic-72 is a positron emitting isotope with promising properties for syntheses of

Cited by 35 publications

References 8 publications

Vascular Imaging of Solid Tumors in Rats with a Radioactive Arsenic-Labeled Antibody that Binds Exposed Phosphatidylserine

Vascular Imaging of Solid Tumors in Rats with a Radioactive Arsenic-Labeled Antibody that Binds Exposed Phosphatidylserine

A no-carrier-added⁷²Se/⁷²As radionuclide generator based on solid phase extraction

Labelling with positron emitters of pnicogens and chalcogens

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A no-carrier-added 72Se/72As radionuclide generator based on distillation

Abstract: Arsenic-72 is a positron emitting isotope with promising properties for syntheses of

Cited by 35 publications

References 8 publications

Vascular Imaging of Solid Tumors in Rats with a Radioactive Arsenic-Labeled Antibody that Binds Exposed Phosphatidylserine

Vascular Imaging of Solid Tumors in Rats with a Radioactive Arsenic-Labeled Antibody that Binds Exposed Phosphatidylserine

A no-carrier-added72Se/72As radionuclide generator based on solid phase extraction

Labelling with positron emitters of pnicogens and chalcogens

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A no-carrier-added ⁷²Se/⁷²As radionuclide generator based on distillation

A no-carrier-added⁷²Se/⁷²As radionuclide generator based on solid phase extraction