A new washing procedure for inorganic element analysis of hair

Verrey, Dominique; Durand, Séverine; Thomas, Olivier; Lelévrier, Vanessa; Quénel, Philippe; Bot, Barbara Le

doi:10.1038/s41370-018-0112-3

Cited by 10 publications

(14 citation statements)

References 38 publications

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“…ICP system has been used to analyze elements in various biological samples including hairs [10]. To collect reliable results, it is required to process a proper pre-treatment step which prevents from any exogenous contamination [5,18]. On the other hand, it is important to keep endogenous elements without losing that allows to measure the actual amount of elements accumulated in samples.…”

Section: Resultsmentioning

confidence: 99%

“…In addition, the comprehensive analysis of untargeted elements has been developed and applied using metallomic analysis [19]. At the same time, it is important to establish the analytical condition of minerals because the comprehensive analysis is affected by the sample preparation process [18,20,21]. This study emphasized the need for the establishment of the hair sample preparation for ICP analysis.…”

Section: Resultsmentioning

confidence: 99%

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Comparison of sample preparation procedures of inductively coupled plasma to measure elements in dog’s hair

Chun¹,

Bang²,

Ji³

et al. 2020

J Anim Sci Technol

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The pre-treatment condition affects on the element analysis of inductively coupled plasma (ICP). In this study the pre-treatment condition of ICP has been studied to quantify elements in dog's hair. The hair samples were collected from twelve female Beagles by clipping them into 1 or 2 cm at the back neck. The qualitative and quantitative analysis of elements in hairs were performed by using ICP. By ICP nine elements were qualitatively detected and quantitatively analyzed (Ca, Cu, Fe, K, Mg, Na, P, Se, Zn). The measured amounts of elements were compared between 3 step and 2 step procedures which were with and without the acetone based washing step. The quantitative analysis showed that the concentrations of K, Na, P, and Se were significantly decreased in hair samples with acetone-based washing (p < 0.005 or 0.001) unlike those without the acetone-based washing. It implied that some minerals are lost by the acetone based washing during the sample preparation step. Therefore, the acetone based washing process is not suitable for quantifying elements in dog's hair. In addition, the results of qualitative and quantitative analysis were compared. Although there was a difference in absolute values of elemental contents in hair, the results of qualitative and quantitative analysis were significantly correlated each other. This finding suggested that the results of qualitative analysis can be used to monitor elemental contents in dog's hair.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Comparison of sample preparation procedures of inductively coupled plasma to measure elements in dog’s hair

Chun¹,

Bang²,

Ji³

et al. 2020

J Anim Sci Technol

View full text Add to dashboard Cite

show abstract

“…However, many studies do not apply this procedure, and have applied various washing procedures, e.g., only water and/or detergent, [11][12][13][14] different organic solvents or mixture of solvents, [15][16][17][18] and acidic solutions at low pH. 19,20 To the best of our knowledge, this is the first study where washing procedures presented in literature were tested alongside one another. The present study aimed to examine the washing procedures for removing external contamination from hair samples before element (As, Ca, Cd, Cu, Fe, Hg, Mg, Mn, Mo, Pb, Se, and Zn) analysis by ICP-MS method.…”

mentioning

confidence: 99%

“…Washing efficiency (% washed) was calculated as the percentage of the difference between the ratio of element concentrations before or after washing, and unwashed value. 19,21 Statistical analysis and graphical illustrations were performed using Microsoft Excel Office Professional 2016 (Microsoft, USA).…”

mentioning

confidence: 99%

“…10 Instead of an organic solvent, several studies used acids to remove external contamination from hair. [19][20] In this study, we evaluated the washing efficiency of different washing procedures reported in the literature for washing hair samples before element analysis. Through the determination of element concentrations in unwashed hair samples, we obtained information on the primary element levels in the hair, which represent the total concentration of the elements, including possible contamination.…”

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confidence: 99%

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Određivanje elemenata u kosi metodom ICP-MS

et al. 2020

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Human hair is a biological sample that is, unlike blood, noninvasively collected and can be used in the assessment of element intake. Before analysis, hair samples need to be washed to eliminate external contamination, for which no standard procedure exists. The present study evaluated the efficiency of different processes for washing hair samples (by non-ionic detergent, acids, solvents, and their mixtures), including ultrasonication before ICP-MS element (As, Ca, Cd, Cu, Fe, Hg, Mg, Mn, Mo, Pb, Se, and Zn) analysis. All tested washing procedures using detergent or solvents were satisfactory, while nitric and hydrochloric acid solutions yielded to “released” and lost elements due to damaged hair (visible discolouration and hair impairment). The application of ultrasonication improved washing efficiency up to 10 %, depending on the tested element and washing procedure.

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