A New Version of Comet Assay

Chernigina, I. A.; Shcherbatyuk, T. G.

doi:10.17691/stm2016.8.1.03

Cited by 4 publications

(3 citation statements)

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“…C ≥ 0-the extended tumors growth, -1 ≤ C < 0-the tumors regression. The level of DNA damage in leukocytes of whole blood of rats was determined with the new version of the COMET assay protocol [40].…”

Section: Methodsmentioning

confidence: 99%

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DNA Damage after Ozone-Photodynamic Therapy in Cancer Animals: Experimental Research

Tatiana¹,

Irina²,

Evgeniya³

et al. 2017

JPP

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Abstract:Objectives: To assess the genotoxic effect of a new antitumor ozone-photodynamic therapy using the improved modification of the COMET assay. Methods: Xenograft cancer models on 58 rats were used. The sarcoma RA was transplanted subcutaneously, and after increasing of tumor volume from 0.5 to 4.2 cm 3 , rats were divided into the four groups: "Intact"-healthy,"Control"-with xenografted tumors and no treatment, "PDT"-the rats treated with the photodynamic therapy, "PDT + ozone"-the rats were treated with both photodynamic therapy and injections of ozonated saline solution. The toxicity of treatment was assessed by DNA damage in leukocytes using the new modification of the COMET assay. The analysis of the "COMETs" was performed following the percentage of DNA in the tail of the "COMET" (% TDNA). Results: A combination of PDT and ozone makes the strongest negative impact on tumor growth. The tumor growth inhibition is associated with low genotoxic exposure of ozone-photodynamic therapy on whole blood leukocytes of cancer rats. Conclusions: A new modification of the COMET assay can provide the assessment of the genotoxic effect of the antitumor therapy in experimental neoplasia.

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Section: Methodsmentioning

confidence: 99%

“…In practice, it is difficult to standardize an impact of radiation. Therefore, we used a new version of the COMET D DAVID PUBLISHING assay that makes it possible to introduce this test to clinical medicine [40].…”

Section: Introductionmentioning

confidence: 99%

DNA Damage after Ozone-Photodynamic Therapy in Cancer Animals: Experimental Research

Tatiana¹,

Irina²,

Evgeniya³

et al. 2017

JPP

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“…The comet assay is a relatively simple tool, but it is more sensitive than other DNA damage assay, economical in use and well validated to measure strand damage in a single cell. It allows to determine the level of DNA damage and repair in non-dividing nucleated cells (Chernigina et al, 2016). The advantages of the comet assay compared to other tests (PCR, HPLC, Micronuclei assay, FCM, immunological assay, etc.)…”

Section: Introductionmentioning

confidence: 99%

Assessment of hOGG1 Genetic Polymorphism (rs1052133) and DNA Damage in Radiation-Exposed Workers

Surniyantoro

Yusuf

Rahardjo

et al. 2022

Asian Pac J Cancer Prev

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Objective:The aim of this study was to assess the effect of radiation exposure, human 8-oxoguanine DNA N-glycosylase-1 (hOGG1) exon 7 genetic polymorphism and confounding factors on DNA damage response. Methods: Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and alkaline Comet assay method were applied to determine the hOGG1 genetic polymorphisms and DNA damage response. A total of 80 participants were enrolled in this study, consisting of 40 radiation-exposed workers as a case group and 40 non-radiation workers as a control group. Result: The genotypes frequencies for controls were Ser/Ser (35%), Ser/Cys (32.5%), and Cys/ Cys (32.5%), with frequencies of alleles being 326Ser (0.52) and 326Cys (0.48), whereas the genotypes frequencies for radiation-exposed workers (cases group) were Ser/Ser (17.5%), Ser/Cys (57.5%), and Cys/Cys (25%), with frequencies of alleles being 326Ser (0.46) and 326Cys (0.54). The results indicated that DNA damage response were not significantly higher in the exposed workers than in controls (22.55 ± 6.02 versus 21.72 ± 7.14; P=0.58). The time of exposure has a significantly negative correlation with comet tail length value among radiation workers. In addition, it was found that the DNA damage response was strongly associated with age and time of exposure with a decrease of 0.6 percent (P-value: 0.008) and 0.58 percent (P-value: 0.009), respectively. Whereas gender, smoking habit, and equivalent dose were not correlated with DNA damage. Conclusion: The single-nucleotide polymorphism of hOGG1 exon 7 (rs1052133) demonstrated no association with the extent of DNA damage in radiation-exposed workers.

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Alkaline Comet Assay as a Predictor of DNA Damage in Medical Radiation Workers

Surniyantoro

Darlina

Rahardjo

2020

J. Phys.: Conf. Ser.

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The study was aimed to examine the deoxyribonucleic acid (DNA) damage level between medical and non-medical radiation workers using the comet assay method. The radiation-exposed group (29 subjects) and the control group (29 subjects) were chosen from the administration staff. The assessment of single-strand DNA damage hosted by peripheral blood lymphocyte was performed using the alkaline comet assay, the tail length and long-tailed DNA were measured to detect the DNA damage. An independent sample t-test was used to assess the statistical difference between groups. The results showed that comet tail length mean measured in the exposed worker’s group was 25.57±4.4 μm whereas the mean percentage of long-tailed DNA was 6.97±1.53. In the control group, the mean tail length was 23.15±7.57 μm and the long-tailed nucleus percentage was 5.65±1.99. Mean values for the tail length measured were no significantly higher in the exposed group compared to the control group (P=0.15), whereas the percentage of long-tailed nuclei was significantly higher in the exposed group compared to the control group (P=0.007) . The observed single-strand DNA can be measured by using the alkaline comet assay. The DNA damage can be influenced by radiation exposure in medical radiation workers.

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A New Version of Comet Assay

Cited by 4 publications

References 0 publications

DNA Damage after Ozone-Photodynamic Therapy in Cancer Animals: Experimental Research

DNA Damage after Ozone-Photodynamic Therapy in Cancer Animals: Experimental Research

Assessment of hOGG1 Genetic Polymorphism (rs1052133) and DNA Damage in Radiation-Exposed Workers

Alkaline Comet Assay as a Predictor of DNA Damage in Medical Radiation Workers

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