Objective:
The purpose of this study was to assess the immune response and malondialdehyde levels in irradiated rats supplemented with
Curcuma xanthorriza
Roxb extract as a candidate for mitigating radiation exposure.
Methods:
Twenty-four male Wistar rats were grouped into eight treatment groups, then Curcuma xanthorrhiza Roxb extract was administered orally and irradiated at 6 Gy. Measurement of rats IL-6 and INF-γ was performed using a sandwich ELISA Kit, while the MDA concentration was quantified according to the method of Wills (1971). The statistical test is determined by one way ANOVA test. P-value <0.05 was considered statistically significant.
Result:
The concentration of IL-6 in all groups showed no statistically significant difference (P=0.18). There was an increase in the concentration of IL-6 in the group of rats irradiated with 6 Gy for 7 days and 14 days. Meanwhile, the INF-γ concentration also showed no significant results in all treatment groups (P=0.28). The average of MDA concentration showed a significant difference in the liver and spleen of irradiated rats at 6 Gy for 14 days compared to the control (0.044 nmol/mg vs 0.008 nmol/mg, P=0.03 and 0.032 nmol/mg vs 0.014 nmol/mg, P=0.05, respectively).
Conclusion:
The administration of
Curcuma xanthorriza
Xorb extract was able to reduce MDA concentrations in the liver and spleen although not statistically significant. In addition, exposure to ionizing radiation at a dose of 6 Gy significantly increased lipid peroxidation in the liver and spleen by 5.5 times and 2.3 times, respectively.
ABSTRAK EVALUASI HUBUNGAN DOSIS RADIASI TERHADAP KERUSAKAN DNA SEL LIMFOSIT DENGAN MENGGUNAKAN TES COMET.Kerusakan asam nukleat (DNA) akibat radiasi atau faktor lain dapat mengarah ke efek yang lebih parah seperti sel kanker. Oleh karena itu diperlukan suatu metode deteksi untuk mengetahui besarnya kerusakan tersebut antara lain dengan metode uji komet yang sederhana. Tujuan penelitian ini adalah mengkaji efek radiasi pada sel limfosit akibat radiasi melalui teknik uji komet. Spesimen darah diiradiasi dosis gamma 2-8 Gy kemudian diisolasi limfositnya dengan histopaque. Isolat limfosit diproses uji komet pada slide dalam kondisi alkali dan hasil pencitraan dianalisis dengan software khusus. Hasil menunjukkan bahwa terdapat korelasi positif parameter komet dengan dosis radiasi, semakin besar dosis paparan semakin besar kerusakan DNA. Diantara parameter uji komet, tail DNA memberikan korelasi yang terbaik dengan dosis radiasi. Disimpulkan bahwa uji komet sangat baik digunakan untuk mengetahui kerusakan DNA akibat radiasi.Kata kunci: kerusakan DNA, radiasi, tes komet, kondisi alkali ABSTRACT EVALUATION OF RADIATION DOSAGE RELATIONSHIP TO DNA CELL DAMAGE USING COMET TEST Nucleic acid (DNA) damage caused by radiation or other factors may lead to more suffering effects such as cancer cells. Therefore it needs a detection method to determine the extent of these damages, one of them is comet test that is a simple method. The Aim of the research was to assess radiation effects on lymphocyte cells caused by radiation using comet test. Blood speciment was irradiated with gamma rays at doses of 2-8 Gy and then lymphocytes were isolated with histopaque. Isolated lymphocyte to proceed comet test in alkali condition and their images were analyzed with special software. Results showed that there is a positive correlation of comet with radiation dose, higher dose caused higher DNA damage. Among parameters of comet test, tail DNA gave the best correlation with dose of radiation. It can be concluded that comet test is a fit method to evaluated radiation induced DNA damage.
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