A new vanilla species from Costa Rica closely related to V. planifolia (Orchidaceae)

Azofeifa-Bolaños, José Bernal; Gigant, L. Rodolphe; Nicolás-García, Mayra; Pignal, Marc; Tavares-González, Fabiola B.; Hágsater, Eric; Salazar, Gerardo A.; Reyes-López, Delfino; Archila-Morales, Fredy L.; García-García, José Antonio; Silva, Denis Da; Allibert, Agathe; Solano-Campos, Frank; Rodrı́guez-Jimenes, G.C.; Paniagua-Vásquez, Amelia; Besse, Pascale; Pérez-Silva, Araceli; Grisoni, Michel

doi:10.5852/ejt.2017.284

Cited by 592 publications

(23 citation statements)

References 31 publications

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“…Ante el desafío de lograr plántulas in vitro libres de contaminación y con un desempeño morfogenético adecuado para la producción comercial, la desinfección efectuada en invernadero y en laboratorio fue efectiva. Para el logro de ese objetivo se tomaron en consideración aspectos inherentes al explante y fisiológicos del material donante, considerados como fundamentales para la selección del diseño experimental en ensayos generales de cultivo in vitro (Trigiano y Gray, 2004); entre ellos, se verificó con exactitud la fidelidad genética de la población (Azofeifa-Bolaños et al, 2017), se incrementó el material en invernadero (ambiente protegido) para aumentar el crecimiento activo de los brotes y disminuir la carga microbiana de las plantas silvestres expuestas a la lluvia y al polvo (George et al, 2008), se utilizó un tamaño de explante inicial diferente a lo indicado en la literatura que propició un adecuado crecimiento, tanto en invernadero como en laboratorio, y se realizó un preacondicionamiento (desinfección) en invernadero en la época más seca del año. Este último procedimiento es fundamental en las plantas que viven en asociación con hongos endófitos (Mng'omba et al, 2012), como es el caso de V. planifolia (Khoyratty et al, 2015).…”

Section: Discussionunclassified

“…Los bancos de germoplasma o programas de mejoramiento implementan técnicas eficientes para la producción vegetal masiva, inmersos en una estrategia global de manejo y aprovechamiento de los recursos fitogenéticos (Soto-Arenas y Solano-Gómez, 2007). La Universidad Nacional de Costa Rica (UNA) lidera un programa en ese sentido, para lo cual ya se ha establecido un banco de germoplasma (con más de 150 accesiones), se han caracterizado especies recolectadas en diversas partes del país (incluida una especie nueva), y se ha recurrido a la multiplicación de material in vitro para reproducir materiales valiosos (Azofeifa-Bolaños et al, 2014;2017).…”

Section: Introductionunclassified

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Desinfección de segmentos nodales sobre el rendimiento morfogenético de vitroplantas de Vanilla planifolia Andrews

et al. 2019

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Introducción. El establecimiento aséptico en condiciones de laboratorio de parientes silvestres de Vanilla planifolia, no ha sido documentado, así como tampoco se ha evaluado la importancia de la desinfección en el aumento del vigor. Objetivo. El objetivo de este trabajo fue evaluar la respuesta morfogenética de vitroplantas de V. planifolia a la desinfección en invernadero y en laboratorio, durante el establecimiento y multiplicación in vitro. Materiales y métodos. El estudio se realizó durante el 2015 y 2016 en el Laboratorio de Cultivo de Tejidos del Instituto de Investigación y Servicios Forestales (INISEFOR) de la Universidad Nacional (UNA), Heredia, Costa Rica. El medio empleado fue el cultivo Murashige y Skoog (MS). Se evaluaron seis tratamientos: 1) alcohol 70%/NaClO 0,35%; 2) kilol/NaClO 0,35%; 3) testigo/NaClO 0,35%; 4) alcohol 70%/HgCl2 0,2%; 5) kilol/HgCl2 0,2% y 6) testigo/HgCl2 0,2%. Las variables que se evaluaron fueron: porcentaje de contaminación total, por bacterias y hongos; longitud, peso y diámetro del brote; número, longitud y peso de las raíces. Resultados. Los géneros de bacterias y hongos más dominantes fueron Pantoea sp. (33%) y Fusarium sp. (12%). El mejor tratamiento de desinfección se obtuvo con el uso de kilol en invernadero y HgCl2 en laboratorio, donde se logró un 100% de explantes libres de contaminantes. Este mismo tratamiento presentó valores significativamente superiores para la longitud y peso del brote, número y peso de las raíces, durante el establecimiento. En la etapa de multiplicación, la condición aséptica de los grupos experimentales presentó valores significativamente mayores para las variables longitud y peso del brote comparado con la respuesta morfogénica presentada por las vitroplantas verdes y vigorosas en la condición de contaminación. Conclusión. La secuencia de tratamientos de desinfección representa la línea base para la optimización de protocolos de cultivo in vitro de parientes silvestres de V. planifolia, constituye un aporte para la conservación ex situ de materiales únicos y sirve para incrementar material genético promisorio.

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Section: Discussionunclassified

Section: Introductionunclassified

Desinfección de segmentos nodales sobre el rendimiento morfogenético de vitroplantas de Vanilla planifolia Andrews

et al. 2019

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“…Costa Rica is located within the native growing ranges of several aromatic vanilla species and has a tradition of small‐scale vanilla cultivation, focusing on the commercial species V. planifolia and more recently the hybrid of Costa Rica [ V. planifolia × V. pompona ] × V. planifolia (Quirós ). Costa Rica's lowland rainforests harbor several wild vanilla species of great interest for their potential in breeding programs, but under‐studied in terms of their biology and ecology (Azofeifa‐Bolaños ). An important area regarding the presence of vanilla CWRs is the Área de Conservación Osa (ACOSA) in the southwest of Costa Rica (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Little is known about the exact distribution of these species, and it is likely that nomenclatural changes are required in this taxonomically challenging genus (Soto‐Arenas and Cribb ). Presently, 14 vanilla species have been reported for Costa Rica ( V. costaricensis, V. dressleri, V. hartii, V. helleri, V. inodora, V. insignis, V. karen‐christianae, V. odorata, V. phaeantha, V. planifolia, V. pompona, V. sarapiquensis, V. sotoarenasii, and V. trigonocarpa ) in addition to the hybrid species mentioned above (Soto Arenas and Dressler , Azofeifa‐Bolaños , Karremans and Lehmann ). Seven of these species ( V. dressleri, V. hartii, V. karen‐christianae, V. odorata, V. planifolia, V. pompona, and V. trigonocarpa ) are found within our study region ACOSA (based on forest inventories carried out within ACOSA), all belonging to the aromatic clade.…”

Section: Methodsmentioning

confidence: 99%

“…A dataset of georeferenced presence locations of vanilla species occurring in Costa Rica was compiled from different sources, including records from online databases such as the Global Information Facility (Global Biodiversity Information Facility ), the Botanical Information and Ecology Network (BIEN; Enquist ) and Tropicos (tropicos.org 2019), scientific articles and reports (among others Soto Arenas and Dressler , Azofeifa‐Bolaños , Karremans and Lehmann ), and forest inventories carried out within our study area and the rest of Costa Rica by ourselves, local universities (Universidad de Costa Rica and Universidad Nacional de Costa Rica), and partner organizations such as Osa Conservation and Ministerio de Ambiente y Energia—Sistema Nacional de Áreas de Conservación (MINAE‐SINAC). When using SDMs, the extent of the modeling range is of particular importance.…”

Section: Methodsmentioning

confidence: 99%

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Vanilla distribution modeling for conservation and sustainable cultivation in a joint land sparing/sharing concept

et al. 2020

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Vanilla, an expensive but popular spice used in many industries, faces problems related to its supply. Some of these problems are due to the fact that vanilla cultivation is based on clonal material of a single species (Vanilla planifolia) and is dominated by just a few countries located outside the native growing areas of aromatic vanilla species, which is the neotropics. Despite the economic importance of this crop, relatively little attention has been paid to its wild relatives, in particular with respect to their biology, ecology, and potential use. We hypothesized that species distribution models (SDMs) can identify suitable areas for both the conservation and cultivation of vanilla crop wild relatives (CWRs), following a joint land sparing/land sharing (SPASHA) approach, thus offering alternative sourcing areas and production methods. This is the first study that explored the use of ensemble SDMs to provide applicable land use maps related to the conservation and sustainable cultivation of wild vanilla species in Costa Rica, contributing to a solution for the problems related to current vanilla production systems. We focused on four aromatic vanilla CWRs, native to Costa Rica, to make land use policy recommendations for this country, and more specifically for the biological corridor Osa and its surroundings within our study region Area de Conservaci on Osa (ACOSA). The resulting distribution maps, with a mean AUC of 0.89, reflected their current potential distribution (ranging from unsuitable to suitable) in Costa Rica. Combining them with recent land use and conservation area maps of our study region, we defined (1) areas for vanilla conservation and (2) areas for sustainable vanilla cultivation within agroforestry systems. These land use recommendations can now be integrated within the National Bio-Corridor Program (PNCB) that aims at making biological corridors more productive by proposing alternative income generation for local communities living within these areas. Our approach can be applied to identify priority areas for implementing the SPASHA approach on other vanilla CWRs and in more regions across its native growing ranges, given the availability of land use maps and enough occurrence records to build accurate SDMs.

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Uncovering haplotype diversity in cultivated Mexican vanilla species

Ellestad

Farrera

Forest

et al. 2022

American J of Botany

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Premise Although vanilla is one of the best‐known spices, there is only a limited understanding of its biology and genetics within Mexico, where its cultivation originated and where phenotypic variability is high. This study aims to augment our understanding of vanilla's genetic resources by assessing species delimitation and genetic, geographic, and climatic variability within Mexican cultivated vanilla. Methods Using nuclear and plastid DNA sequence data from 58 Mexican samples collected from three regions and 133 ex situ accessions, we assessed species monophyly using phylogenetic analyses and genetic distances. Intraspecific genetic variation was summarized through the identification of haplotypes. Within the primarily cultivated species, Vanilla planifolia, haplotype relationships were further verified using plastome and rRNA gene sequences. Climatic niche and haplotype composition were assessed across the landscape. Results Three species (Vanilla planifolia, V. pompona, and V. insignis) and 13 haplotypes were identified among Mexican vanilla. Within V. planifolia haplotypes, hard phylogenetic incongruences between plastid and nuclear sequences suggest past hybridization events. Eight haplotypes consisted exclusively of Mexican samples. The dominant V. planifolia haplotype occurred throughout all three regions as well as outside of its country of origin. Haplotype richness was found to be highest in regions around Papantla and Chinantla. Conclusions Long histories of regional cultivation support the consideration of endemic haplotypes as landraces shaped by adaptation to local conditions and/or hybridization. Results may aid further genomic investigations of vanilla's genetic resources and ultimately support the preservation of genetic diversity within the economically important crop.

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A new vanilla species from Costa Rica closely related to V. planifolia (Orchidaceae)

Abstract: D N A L i b r a r y o f L i f e , r e s e a r c h a r t i c l e 1A new vanilla species from Costa Rica closely related to V. planifolia (Orchidaceae) José

Cited by 592 publications

References 31 publications

Desinfección de segmentos nodales sobre el rendimiento morfogenético de vitroplantas de Vanilla planifolia Andrews

Desinfección de segmentos nodales sobre el rendimiento morfogenético de vitroplantas de Vanilla planifolia Andrews

Vanilla distribution modeling for conservation and sustainable cultivation in a joint land sparing/sharing concept

Uncovering haplotype diversity in cultivated Mexican vanilla species

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