A new UV‐B absorbing mycosporine with photo protective activity from the lichenized ascomycete Collema cristatum

Abstract

A novel photo protective mycosporine was isolated from the lichenized ascomycete Collema cristatum. Biological activity was measured in terms of protection against UV‐B induced membrane destruction and pyrimidine dimer formation in cultured human keratinocytes, and prevention of UV‐B induced erythema. It was found that the pure isolated compound prevented UV‐B induced cell destruction in a dose‐dependent manner, that the compound partially prevented pyrimidine dimer formation and completely prevented UV‐B induced erythema when applied to the skin prior to irradiation.

Natural microbial UV radiation filters — Mycosporine-like amino acids

“…The mycobiont was cultivated and biological activity was measured in terms of protection against UV-B induced membrane destruction and pyrimidine dimer formation in cultured human keratinocytes, and prevention of UV-B-induced erythema. The pure isolated compound was found to prevent UV-B-induced cell destruction in a dosedependent manner, it partially prevented pyrimidine dimer formation, and completely prevented UV-B-induced erythema when applied to the skin prior to irradiation (Enk et al 2002;Torres et al 2004).…”

Analysis of Lichen Metabolites, a Variety of Approaches

“…Then, mycosporines, more recently reported in lichens (Torres et al 2004), are characterized through their UV spectrum absorbing in the 310-365 nm range with a strong molar extinction coefficient and a symmetrical shape. Detection through analytical techniques hyphenated with a PDA spectrophotometer offers a simple and easy method to screen them from lichen extracts (Roullier et al 2011).…”

“…Other than specific and unique lichen metabolites, various other structural families can be recognized: aliphatic acids (roccellic acid) and related lactones, xanthones (lichexanthone), chromones (siphulin), pulvinic acid derivatives (pulvinic acid dilactone), quinones (haemoventosin, parietin), terpenes (zeorin), steroids (ergosterol peroxide), and carotenoids (astaxanthin). Cyanolichens mostly contain sugars and amino acid derivatives although original compounds such as mycosporines were detected recently (Torres et al 2004;Roullier et al 2011).…”

Biodiversity of the Chemical Constituents in the Epiphytic Lichenized Ascomycete Ramalina Lacera Grown on Difference Substrates Crataegus Sinaicus, Pinus Halepensis, and Quercus Calliprinos

“…Orsellinic and usnic acids were obtained from Sigma-IL, and used also as standard compounds. Isolated metabolites were identifi ed with 13 C-NMR, IR, UV, and chemical methods as described previously 11 .…”

“…These organisms have both algal and fungal properties and produce n-alkanes 5 , unusual betaine ether glycerols 6 , glyco-and phospholipids 7,8 , and saturated, unsaturated, branched 8,9 , and halogenated fatty acids 10 . Many diff erent bioactive secondary metabolites have also been isolated from lichen species [11][12][13] which have been used in pharmaceutical and biotechnological sciences and industry [14][15][16] . Some Ramalina species are usually used as food in some Central and South Eastern Asian countries.…”