A new ultrasensitive way to circumvent PCR-based allele distinction: Direct probing of unamplified genomic DNA by solution-phase hybridization using two-color fluorescence cross-correlation spectroscopy

Földes-Papp, Zeno; Kinjo, Masataka; Tamura, Mamoru; Birch-Hirschfeld, Eckhard; Demel, Ulrike; Tilz, Gernot P.

doi:10.1016/j.yexmp.2005.01.005

Cited by 26 publications

(19 citation statements)

References 32 publications

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“…(2)), have recently been incorporated with SMD for the detection of nucleic acids [21,29,38] and point mutations [14]. We have demonstrated a dual-color SMD assay for comparative quantification of specific nucleic acids using molecular beacons ( Fig.…”

Section: Fret-based Methodsmentioning

confidence: 99%

“…In the last decade, several assay methods for nucleic acid detection based on fluorescence correlation spectroscopy (FCS) have been reported [16,18,38,[40][41][42][43][44][45][46][47][48][49]. The principle of FCS lies in temporal and spatial fluorescence fluctuation due to molecules diffusing in and out of the small detection volume.…”

Section: Fluorescence Correlation Spectroscopy (Fcs)mentioning

confidence: 99%

“…Recently, several schemes for fluorescence background reduction based on FRET have been reported. The use of hairpin molecular beacon probes instead of linear probes in dualcolor FCS has been shown to decrease of background by a factor of 40 to 100 [38]. The significant reduction in background, as a result, makes dual-color FCS amenable to the analysis of low-abundance, unamplified targets.…”

Section: Fluorescence Correlation Spectroscopy (Fcs)mentioning

confidence: 99%

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Single-Molecule Detection and Probe Strategies for Rapid and Ultrasensitive Genomic Detection

Yeh¹,

Chao²,

Ho³

et al. 2005

CPB

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This paper reviews the current state-of-the-art development of single-molecule detection (SMD)-based methods for ultrasensitive and specific analysis of genomic sequences. We first discuss several newly devised single fluorescent probe strategies that allow separation-free detection of low-abundance DNA sequences, such as quantum dot (QD)-mediated fluorescence resonance energy transfer (FRET) technology and dual-color fluorescence coincidence and colocalization analysis. Various schemes toward single DNA sizing and sequencing in solutions or on surfaces are also reviewed. In the end, we summarize the different microfluidic approaches developed for use with SMD to facilitate rapid, low-volume and quantitative analysis, such as electrokinetic and hydrodynamic single-molecule manipulation techniques.

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Section: Fret-based Methodsmentioning

confidence: 99%

Section: Fluorescence Correlation Spectroscopy (Fcs)mentioning

confidence: 99%

Section: Fluorescence Correlation Spectroscopy (Fcs)mentioning

confidence: 99%

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Single-Molecule Detection and Probe Strategies for Rapid and Ultrasensitive Genomic Detection

Yeh¹,

Chao²,

Ho³

et al. 2005

CPB

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“…Wabuyele et al (2003) demonstrated a rapid detection scheme capable of detecting rare point mutation in unamplified genomic DNA using singlemolecule fluorescent burst detection [11]. Foldes-Papp et al (2005) demonstrated direct probing of unamplified genomic DNA in solution down to femtomolar allele concentrations using two-color fluorescence cross-correlation spectroscopy [12].…”

Section: Introductionmentioning

confidence: 99%

Magnetic Modulation Biosensing for Rapid and Homogeneous Detection of Biological Targets at Low Concentrations

Danielli¹,

Porat²,

Ehrlich³

et al. 2010

CPB

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This paper reviews the development of a magnetic modulation biosensing (MMB) system for rapid, simple and sensitive detection of biological targets in homogeneous solution at low concentrations. It relies on condensation and modulation of the fluorescent-labeled probes attached to magnetic beads using an alternating magnetic field gradient. Condensation of the beads from the entire volume increases the signal while modulation separates the signal from the background noise of the non-magnetized solution. We first discuss the motivation and challenges in specific DNA sequences detection as well as current approaches to overcome some of these challenges. We then present the MMB system, DNA detection schemes and magnetic beads manipulation in solution. Rapid detection at sub-picomolar concentrations of fluorescent-labeled probes as well as of coding sequences of the non-structural Ibaraki virus protein 3 (NS3) complementary DNA (cDNA) without any washing or separation step is also reviewed. Finally, we show preliminary results of protein detection using a 'sandwich'-based assay.

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“…Another approach also uses fluorescent dyes; however, the detection sensitivity is improved by time-resolved fluorescence detection (e.g. single molecule fluorescence burst detection (Wabuyele et al, 2003), two-color fluorescence cross-correlation spectroscopy (Foldes-Papp et al, 2005)). A third approach uses metallic or semiconductor nanoparticles for biosensing (Willner et al, 2007) is either electrical (Park et al, 2002) or optical (Nam et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Rapid homogenous detection of the Ibaraki virus NS3 cDNA at picomolar concentrations by magnetic modulation

Danielli

Porat

Arie

et al. 2009

Biosensors and Bioelectronics

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A new ultrasensitive way to circumvent PCR-based allele distinction: Direct probing of unamplified genomic DNA by solution-phase hybridization using two-color fluorescence cross-correlation spectroscopy

Cited by 26 publications

References 32 publications

Single-Molecule Detection and Probe Strategies for Rapid and Ultrasensitive Genomic Detection

Single-Molecule Detection and Probe Strategies for Rapid and Ultrasensitive Genomic Detection

Magnetic Modulation Biosensing for Rapid and Homogeneous Detection of Biological Targets at Low Concentrations

Rapid homogenous detection of the Ibaraki virus NS3 cDNA at picomolar concentrations by magnetic modulation

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