Rapid homogenous detection of the Ibaraki virus NS3 cDNA at picomolar concentrations by magnetic modulation

Danielli, Amos; Porat, Noga; Arie, Ady; Ehrlich, Marcelo

doi:10.1016/j.bios.2009.08.047

Cited by 33 publications

(29 citation statements)

References 19 publications

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“…Recently, a magnetic modulation-biosensing (MMB) system for rapid and homogeneous detection of Ibaraki virus NS3 cDNA was developed by Danielli et al [94]. The motion of streptavidin-coupled superparamagnetic beads was controlled by an alternating magnetic field.…”

Section: Magnetic Manipulationmentioning

confidence: 99%

Recent advances in biological detection with magnetic nanoparticles as a useful tool

Wang

Xiong

et al. 2015

Sci. China Chem.

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Section: Magnetic Manipulationmentioning

confidence: 99%

Recent advances in biological detection with magnetic nanoparticles as a useful tool

Wang

Xiong

et al. 2015

Sci. China Chem.

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“…(2)). A comprehensive description of the system set-up as well as the magnetic pole fabrication can be found in previous papers [20,21]. In order to maneuver the magnetic beads in a periodic 1-D movement we used a homemade current modulator.…”

Section: Magnetic Modulation Biosensing Sys-temmentioning

confidence: 99%

“…Recently, we experimentally demonstrated a novel biosensing method that aims to overcome these drawbacks [20,21]. It relies on condensation and modulation of the fluorescent-labeled probes that are coupled to magnetic beads using an external magnetic field gradient.…”

Section: Introductionmentioning

confidence: 99%

Magnetic Modulation Biosensing for Rapid and Homogeneous Detection of Biological Targets at Low Concentrations

Danielli¹,

Porat²,

Ehrlich³

et al. 2010

CPB

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This paper reviews the development of a magnetic modulation biosensing (MMB) system for rapid, simple and sensitive detection of biological targets in homogeneous solution at low concentrations. It relies on condensation and modulation of the fluorescent-labeled probes attached to magnetic beads using an alternating magnetic field gradient. Condensation of the beads from the entire volume increases the signal while modulation separates the signal from the background noise of the non-magnetized solution. We first discuss the motivation and challenges in specific DNA sequences detection as well as current approaches to overcome some of these challenges. We then present the MMB system, DNA detection schemes and magnetic beads manipulation in solution. Rapid detection at sub-picomolar concentrations of fluorescent-labeled probes as well as of coding sequences of the non-structural Ibaraki virus protein 3 (NS3) complementary DNA (cDNA) without any washing or separation step is also reviewed. Finally, we show preliminary results of protein detection using a 'sandwich'-based assay.

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“…The fluorescent proteins, which are attached to the magnetic beads are condensed into the detection area and their movement in and out of an orthogonal laser beam produces a periodic fluorescent signal that is demodulated using synchronous detection. The magnetic modulation biosensing system was previously used to detect the coding sequences of the non-structural Ibaraki virus protein 3 (NS3) complementary DNA (cDNA) [2]. The techniques that are demonstrated in this work for external manipulation and condensation of particles may be used for other applications, e.g.…”

Section: Introductionmentioning

confidence: 99%

“…

AbstractA magnetic modulation biosensing system (MMB) [1,2] rapidly and homogeneously detected biological targets at low concentrations without any washing or separation step. When the IL-8 target was present, a 'sandwich'-based assay attached magnetic beads with IL-8 capture antibody to streptavidin coupled fluorescent protein via the IL-8 target and a biotinylated IL-8 antibody.

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mentioning

confidence: 99%

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Danielli

Porat

Ehrlich

et al. 2010

JoVE

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A magnetic modulation biosensing system (MMB) [1,2] rapidly and homogeneously detected biological targets at low concentrations without any washing or separation step. When the IL-8 target was present, a 'sandwich'-based assay attached magnetic beads with IL-8 capture antibody to streptavidin coupled fluorescent protein via the IL-8 target and a biotinylated IL-8 antibody. The magnetic beads are maneuvered into oscillatory motion by applying an alternating magnetic field gradient through two electromagnetic poles. The fluorescent proteins, which are attached to the magnetic beads are condensed into the detection area and their movement in and out of an orthogonal laser beam produces a periodic fluorescent signal that is demodulated using synchronous detection. The magnetic modulation biosensing system was previously used to detect the coding sequences of the non-structural Ibaraki virus protein 3 (NS3) complementary DNA (cDNA) [2]. The techniques that are demonstrated in this work for external manipulation and condensation of particles may be used for other applications, e.g. delivery of magnetically-coupled drugs in-vivo or enhancing the contrast for in-vivo imaging applications. Protocol IL-8 assay:MMB system: Representative Results:Visual inspection of the aggregated beads presented a distinct difference between the reaction with the target and the reaction without the target. In all the reactions with the target, the beads formed a single, dense aggregate that moved in and out the laser beam in a unite manner. However, in all the reaction without the target, the beads were less aggregated and their motion was less unite (see Figure 1 3. The reactions are later placed without any separation or washing step in the cuvettes and inspected using the MMB system. 1. Place the cuvette in its position between the two electromagnetic poles. 2. Operate the modulation current and wait 30 seconds to allow aggregation and condensation of the beads 3. Measure the signal of the lock-in amplifier using an oscilloscope.

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Rapid homogenous detection of the Ibaraki virus NS3 cDNA at picomolar concentrations by magnetic modulation

Cited by 33 publications

References 19 publications

Recent advances in biological detection with magnetic nanoparticles as a useful tool

Recent advances in biological detection with magnetic nanoparticles as a useful tool

Magnetic Modulation Biosensing for Rapid and Homogeneous Detection of Biological Targets at Low Concentrations

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

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