A new tool for differentiating hepatocellular cancer cells: Patterned carbon nanotube arrays

Abstract

We aimed to develop a new approach to detect the invasiveness and metastatic degree of hepatocellular carcinoma cells (HCC) based on their epithelial mesenchymal transition (EMT) status by using patterned carbon nanotubes (CNT) without any further surface functionalization. We used well differentiated HUH7 and poorly differentiated SNU182 cells to examine and compare their adhesive features on patterned CNTs. We found that the well differentiated HUH7 cells attached significantly more on the patterned CNTs than the poorly differentiated SNU182 cells due to the difference in epithelial and mesenchymal phenotypes of these cells. Collagen coated patterned CNTs having less roughness resulted in a decrease in the number of attached cells compared to non-coated patterned surfaces indicating that surface topography playing also a vital role on the cell attachment. LDH testing indicated no adverse, or thereof toxic effect of collagen coated or non-coated patterned surfaces on the HCC cells. The results of this study clearly suggest that patterned CNT surfaces can be used as a diagnostic tool to determine the invasiveness and metastatic level of HCCs. Hence, CNTs could be considered as a promising diagnostic tool for the detection of differentiation and invasiveness of the HCC cells. © 2015 Elsevier B.V. All rights reserved

In vitrotoxicity of carbon nanotubes: a systematic review

“…The listed studies in Table 1 can be divided into four main groups according to the used cells types: cells related to nervous system, 14,96,98–109 osteoblasts, 81,83,84,96,110–112 fibroblasts, 84,96,107,111,113–119 and other types. 84,97,119–127…”

“…84,96,97 The listed studies in Table 1 can be divided into four main groups according to the used cells types: cells related to nervous system, 14,96,[98][99][100][101][102][103][104][105][106][107][108][109] osteoblasts, 81,83,84,96,[110][111][112] broblasts, 84,96,107,111,[113][114][115][116][117][118][119] and other types. 84,97,[119][120][121][122][123][124][125][126][127] In the case of neurons, glia or cortical cultures growth, each study demonstrated a successful outcome. Thus, in 2000 the rst attempts were made to study the interface between CNTs and living systems.…”

Nanotechnological advances in cancer: therapy a comprehensive review of carbon nanotube applications

“…In another study, it was found that due to the invasive properties of hepatocellular carcinoma cells highly differentiated cells (HUH7) have a better chance of attachment than poorly differentiated cells (SNU182). Using this property Kucukayan-Dogu et al ( Kucukayan-Dogu et al, 2015 ) developed an imageable carbon nanotube surface and analyzed the ability of differentiated cells to attach to the imageable carbon nanotube surface. It can be used to diagnose the level of invasion of liver ( Figure 3A ) cancer cells.…”