A New Sulfated Triterpene Saponin from Gypsophila trichotoma

Abstract

A new sulfated triterpeniod saponin, 3-O-sulfooleanolic acid 28-O-[β-glucopyranosyl-(1→3)]-[β-glucopyranosyl-(1→6)]-β-glucopyranosyl ester (1), along with three known Δ 7-sterols: stigmast-7-en-3β-ol (2), stigmast-7-en-3-O-β-D-glucopyranoside (3) and stigmast-7-en-3-on (4) were isolated from the roots of Gypsophila trichotoma Wend. (Caryophyllaceae). Their structures were elucidated by chemical and spectral methods. Compound 1 caused concentrationdependent inhibition of malignant cell proliferation against different human tumor cell lines.

Production of Δ7-Sterols from In Vitro Root Cultures of Endangered Gypsophila trichotoma

“…These were purified via recrystallization. Structural assessment of the glycoside was carried out by acid hydrolysis, followed by TLC with authentic reference substances and for all sterols -MS and 1 H and 13 C NMR spectroscopic experiments were performed, as previously described in [4].…”

“…The plant was introduced for in vitro cultivation at the Department of Pharmacognosy of the Faculty of Pharmacy in Sofia [3]. Rare ∆ 7 -sterols (Figure 1), previously isolated from the roots of the plant [4], have been reported to possess antitumor activity. They also have a positive effect in cases of prostate hyperplasia [5].…”