A New Strategy for the Therapy of Pancreatic Cancer Invasion and Metastasis by Protease Inhibitor and Protein Pump Inhibitor Agents.

The proton pump inhibitor inhibits cell growth and induces apoptosis in human hepatoblastoma

“…Moreover, our observations by electron microscopy confirmed apoptosis as the cause of this variation. The same finding has been confirmed by Ohta et al [19] in pancreatic cancer cells and by Nakashima et al [20] in gastric cancer cells. We also confirmed it in our own quantitative evaluation of apoptosis using flow cytometry (a significant increase of apoptotic cells was observed in the Baf-A1-treated hepatoblastoma cell group).…”

“…They also found that the bafilomycin-sensitive ATP hydrolysis system is significantly more prevalent in metastatic breast cancer cells than in non-metastatic ones, and the plasma membrane V-ATPase expression is an essential factor in determining the level of invasiveness of a cancer cell and the acquisition of metastatic properties [22]. Ohta et al [2,19] found ATP6L, a subunit of V-ATPase, in pancreatic cancers, and concluded that it was expressed in invasive pancreatic cancers and not in non-invasive ones. We did not observe the expression of ATP6L, a subunit of VATPase, in our own RT-PCR experiment with hepatoblastoma cells.…”

Bafilomycin A1 induces apoptosis in the human pancreatic cancer cell line Capan-1

“…Our recent studies have demonstrated that V-ATPases are overexpressed in almost all human pancreatic ductal cancer tissues and many human pancreatic cancer cell lines, including Capan-1 cells. 8,9 In this study, we examined whether bafilomycin A 1 can inhibit the growth of Capan-1 human pancreatic cancer cells through induction of apoptosis.…”

Effects of bafilomycin A1, a vacuolar type H+ATPase inhibitor, on the thermosensitivity of a human pancreatic cancer cell line

“…Ohta et al [7,8] previously reported that the vacuolar type H þ ATPases are over-expressed in AsPC-1 cells. The cells were maintained routinely in RPMI 1640 medium containing L-glutamine, 10% foetal bovine serum (GIBCO BRL) and antibiotics at 37 C in a 5% CO 2 incubator.…”