“…It binds within the equatorial region of the V 0 rotor subunit c (6). There are reports on effects of V-ATPase inhibitors including archazolid on tumor growth and invasion (7)(8)(9); however, detailed investigations into the underlying molecular mechanism and signaling pathways are missing. In this respect archazolid could serve as a helpful pharmacological tool to decipher the role of V-ATPase in cell death * This work was supported by the Deutsche Forschungsgemeinschaft Grant FOR 2 The abbreviations used are: V-ATPase, vacuolar H ϩ ATPase; AMPK, AMPactivated protein kinase; ⌬m, mitochondrial potential; HIF1␣, hypoxiainducible factor-1␣; HMEC, human mammary endothelial cell; HUVEC, human umbilical vein endothelial cell; JC-1, 5,5Ј,6,6Ј-tetrachloro-1,1Ј,3,3Ј-tetraethylbenzimidazol-carbocyanine iodide; 3MA, 3-methyladenine; mTOR, mammalian target of rapamycin; NBDG, 2-[N- (7-…”