A new species of Leptolalax (Anura: Megophryidae) from Vietnam

Abstract

We describe a new, medium-sized Leptolalax species from north central Vietnam. Leptolalax puhoatensis sp. nov. is distinguished from its congeners by a combination of having a body size range of 24.2-28.1 mm in eight adult males and 27.3-31.5 mm in three adult females; distinct dorsolateral markings including blackish spots on the flank and dark canthal and/or temporal streaks; males with a reddish-brown venter, often with faint white dusting and females with a pale pink venter; skin on dorsum with tiny, indistinct, low tubercles in preservative, more distinct and forming low dorsal ridges on dorsal surface in life; toes with webbing basal and narrow lateral fringes; iris copper in upper half and golden in lower half; and a call consisting of a single note and a dominant frequency of 4.9-5.6 kHz (at 22.3-25.8º C). Uncorrected sequence divergences between L. puhoatensis sp. nov. and all homologous 16S rRNA sequences available for known species in the genus are ≥6.3%.

Molecular, morphological and acoustic assessment of the genus Ophryophryne (Anura, Megophryidae) from Langbian Plateau, southern Vietnam, with description of a new species

“…460–500 bp fragment of the 16S rRNA gene was sequenced for molecular identification purposes. 16S rRNA is a molecular marker widely applied for biodiversity surveys in amphibians (Vences et al 2005a, 2005b, Vieites et al 2009), and has proven to be particularly useful in studies of megophryid diversity (Matsui et al 2010, 2014, Ohler et al 2011, Stuart et al 2011, 2012, Hamidy et al 2012, Rowley et al 2010a, 2011a, 2012, Jiang et al 2013, Poyarkov et al 2015a and references therein). Amplification was performed in 25 μl reactions using either ca.…”

A new species of the genus Leptolalax (Anura: Megophryidae) from Mt. Gaoligongshan of western Yunnan Province, China

“…Sex was determined by direct observation of calling in life and the presence of internal vocal sac openings, and the presence of eggs in abdomen. Comparative morphological data of Leptolalax species were obtained from examination of museum specimens (see Appendix) and from the literature: L. aereus (Rowley et al 2010c), L. alpinus (Fei et al 2009), L. applebyi (Rowley & Cao 2009), L. arayai (Matsui 1997), L. bourreti (Ohler et al 2011), L. bidoupensis (Rowley et al 2011), L. botsfordi (Rowley et al 2013); L. croceus (Rowley et al 2010a), L. dringi (Inger et al 1995;Matsui & Dehling 2012) , L. lateralis (Anderson 1871;Humtsoe et al 2008), L. laui (Sung et al 2014), L. liui (Fei et al 2009, Sung et al 2014, L. marmoratus (Matsui et al 2014b), L. maurus (Inger et al 1997), L. melanoleucus (Matsui 2006), L. melicus (Rowley et al 2010b), L. minimus (Taylor 1962;Ohler et al 2011), L. nahangensis (Lathrop et al 1998), L. nokrekensis (Mathew & Sen 2010), L. nyx (Ohler et al 2011), L. oshanensis (Fei et al 2009), L. pelodytoides (Boulenger 1893;Ohler et al 2011), L. pictus (Malkmus 1992), L. platycephalus (Dehling 2012a), L. pluvialis (Ohler et al 2000(Ohler et al , 2011, L. pyrrhops (Poyarkov et al 2015), L. sabahmontanus…”

A new species of Leptolalax (Anura: Megophryidae) from northern Vietnam

“…The genus Leptolalax (Dubois 1983) currently contains 29 species of small frogs of the forest floor, distributed in rocky streams in hilly evergreen forest throughout Southeast Asia, southern China and northeastern India (Frost 2010, Rowley et al 2010a, 2010b, 2010c. There has been a rapid increase in the number of known Leptolalax species in recent years, resulting from a combination of increased field surveys in the region and the incorporation of acoustic and molecular data in delineating species boundaries (eg.…”

“…There has been a rapid increase in the number of known Leptolalax species in recent years, resulting from a combination of increased field surveys in the region and the incorporation of acoustic and molecular data in delineating species boundaries (eg. Das et al 2010;Rowley et al 2010aRowley et al , 2010bRowley et al , 2010cSengupta et al 2010).…”

“…To date, eleven species of Leptolalax have been reported from Indochina; L. aereus, L. applebyi, L. bourreti, L. croceus, L. melicus, L. nahangensis, L. oshanensis, L. pelodytoides, L. pluvialis, L. sungi and L. tuberosus (Frost 2010;Rowley et al 2010aRowley et al , 2010bRowley et al , 2010c. All but L. aereus and L. melicus have been reported from Vietnam (Nguyen et al 2009;Rowley et al 2010aRowley et al , 2010c.…”