The knowledge about variation of antioxidant properties from local medicinal plant can be achieved by investigating all natural habitats of it. To reach this goal, a comprehensive survey on eight populations of Allium paradoxum (M. B.) G. Don, was conducted in the Hyrcanian forests. A. paradoxum (Amaryllidaceae) is a perennial, local plant, native to the northern Iran. Different parts of it, is largely used in food preparation and traditional medicine. Plants were collected randomly from different altitudes and forest sites of Iranian northern provinces ranging from west to east (Guilan, Mazandaran and Golestan Provinces). Samples were divided into aerial and bulbous parts. The antioxidant activities of the extracts were investigated with 1, 1-diphenyl-2-picrylhydrazyl (DPPH) and reducing power assays. Total flavonoid content was determined by a colorimetric aluminum chloride method. The highest antioxidant activities and flavonoid contents in A. paradoxum were related to Varaki and Zarinabad sites (Mazandaran province) with relatively higher humidity, in the central part of the Hyrcanian area and in altitudinal range between 462-860 m asl. The content of total phenolics in the extracts was determined according to the Folin-Ciocalteu reagent method, and calculated as gallic acid equivalents (GAE). With respect to total phenolic contents, aerial parts of plants of Jahannama, the lowest elevation site (Golestan province), had the highest amount. Elemental composition (Fe ++ , Mn ++) and total sulphur were also determined using Atomic Absorption Spectroscopy and digestion method, respectively. Higher contents of two elements, particularly Fe, and total sulphur can be found in bulbous part of Zarinabad and Kiasar populations (Mazandaran province) compared to other sites. The results of the current study indicated that there is a remarkable and significant variation of antioxidant activities among different studied populations of A. paradoxum in the Hyrcanian forests.