A New Serum Antibody Test Kit (E Plate) for Evaluation of Helicobacter Pylori Eradication.

Abstract

Objective Serological antibody test have been widely performed to detect the presence ofH. pylori, but they have not been used to evaluate the status of H. pylori after eradication. In this study we evaluated the diagnostic accuracy of a new serological test kit (E-plate) after eradication. Method Eradication ofH. pylori was performed in 100 patients by proton pump inhibitor (PPI)+amoxicillin (AMPC)+clarithromycin (CAM) or PPI+AMPCtherapy. Evaluation of H. pylori was done by culture, histology and rapid urease test before, and 8 weeks after, the treatment. Serological tests were also performed before and after treatment using the E plate. Cure was defined as no evidence of H. pylori at 8 weeks after the treatment. Receiver operating characteristic (ROC) analysis was performed to determine the ideal cut-off value for percentage change in the serological test.Result Success was obtained in 73 patients, failure in 20 patients and there were 7 dropouts. Serological test value was significantly decreased after treatment (44.3±29.6 U/ ml) compared to before treatment (94.8±73.2 U/ml) in the successful cases. In contrast, those with no significant change after treatment (62.7±31.3 U/ml) compared to before treatment (72.9±47.7 U/ml) were considered as failure cases. ROCanalysis revealed that cut-off values of a 20%, 30%, and 40%decrease on E plate result yielded a sensitivity of 95.5%, 92.4%, 71.2% and a specificity of73.3%, 84.2% , 94.7% , respectively. Conclusion The new E plate serological test kit for H, pylori was useful for distinguishing success from failure 8 weeks after completion of eradication therapy for H. pylori. (Internal Medicine 41: 780-783, 2002)

ABC screening for gastric cancer is not applicable in a Japanese population with high prevalence of atrophic gastritis

“…Serum samples were obtained and stored at -20°C and were tested for the prevalence of the IgG antibody to H. pylori by enzyme immunoassay with an E-plate (Eiken, Tokyo, Japan), which uses antigens from the Japanese H. pylori strain [10]. The levels of PG I and PG II were also measured, by radioimmunoassay.…”

Helicobacter Pylori Infection is a Significant Risk for Modified Lipid Profile in Japanese Male Subjects

“…The anti-Helicobacter pylori antibody concentration was measured using an enzyme immunoassay (E plate; Eiken Chemical, Tokyo) 23) ; an assay value 10 U/mL was considered negative and ≥ 10 U/mL was considered positive.…”

бH pylori�� seropositivity and cytokine gene polymorphisms

“…The anti-H pylori antibody titer was measured using an enzyme immunoassay (E plate; Eiken Chemical, Tokyo, Japan) [29] ; an assay value < 10 U/mL was considered neg ative and a value > 10 U/mL was considered positive.…”