A new semiautomatic device with horizontal developing chamber for gradient thin-layer chromatography

Hałka-Grysińska, Aneta; Dzido, Tadeusz H.; Sitarczyk, Ewelina; Klimek-Turek, Anna; Chomicki, Adam

doi:10.1080/10826076.2016.1163177

Cited by 5 publications

(2 citation statements)

References 16 publications

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“…Concentrating zone plates are also likely to reduce the potential dispersion effects if the streak pattern is not perfectly straight. The need for manual handling in the development process can likely be eliminated by integrating the above sample deposition approaches with commercial or custom horizontal TLC setups [ 51 , 52 , 53 , 54 ]. Commercially available online extraction systems also exist for the collection of identified product bands directly from TLC plates without the need for scraping (e.g., CAMAG TLC-MS Interface 2 [ 55 ], Advion Plate Express [ 56 ]), using methods such as liquid extraction.…”

Section: Discussionmentioning

confidence: 99%

Rapid Purification and Formulation of Radiopharmaceuticals via Thin-Layer Chromatography

et al. 2022

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Before formulating radiopharmaceuticals for injection, it is necessary to remove various impurities via purification. Conventional synthesis methods involve relatively large quantities of reagents, requiring high-resolution and high-capacity chromatographic methods (e.g., semi-preparative radio-HPLC) to ensure adequate purity of the radiopharmaceutical. Due to the use of organic solvents during purification, additional processing is needed to reformulate the radiopharmaceutical into an injectable buffer. Recent developments in microscale radiosynthesis have made it possible to synthesize radiopharmaceuticals with vastly reduced reagent masses, minimizing impurities. This enables purification with lower-capacity methods, such as analytical HPLC, with a reduction of purification time and volume (that shortens downstream re-formulation). Still, the need for a bulky and expensive HPLC system undermines many of the advantages of microfluidics. This study demonstrates the feasibility of using radio-TLC for the purification of radiopharmaceuticals. This technique combines high-performance (high-resolution, high-speed separation) with the advantages of a compact and low-cost setup. A further advantage is that no downstream re-formulation step is needed. Production and purification of clinical scale batches of [18F]PBR-06 and [18F]Fallypride are demonstrated with high yield, purity, and specific activity. Automating this radio-TLC method could provide an attractive solution for the purification step in microscale radiochemistry systems.

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Section: Discussionmentioning

confidence: 99%

Rapid Purification and Formulation of Radiopharmaceuticals via Thin-Layer Chromatography

et al. 2022

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“…Additive manufacturing has become an integral part of open-source, low-cost, and self-manufactured operational steps. − However, it has hardly been used for building complete chromatographic systems . Even beyond that, 2LabsToGo systems have not yet been demonstrated.…”

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confidence: 99%

2LabsToGo─Recipe for Building Your Own Chromatography Equipment Including Biological Assay and Effect Detection

et al. 2022

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A complete recipe for building your own chromatography equipment from readily available materials is introduced. It combines sample separation (chemistry laboratory) with biological effect detection (biology laboratory). This hyphenation of two disciplines is necessary for prioritizing important compounds in complex samples. Among the thousands of compounds therein, it is often not clear which compounds are the important ones. On the same separation surface, additional detection of biological effects enables and guides substance prioritization. The newly developed open-source 2LabsToGo system for chemical and biological analysis is completely solvent-resistant and, due to miniaturization, environmentally friendly regarding the consumption of materials. It produces comparable results but is 10 times more compact (26 cm × 31 cm × 34 cm), 10 times lighter (6.8 kg), and 55 times less expensive (€ 1717) than current sophisticated commercial devices. As a proof of concept of the first 2LabsToGo system, the quality of different water samples was analyzed since clean water is becoming increasingly rare. In water, most of the thousands of substance signals or features can neither be identified nor classified toxicologically. However, methods that exploit this hyphenated strategy provide answers to such essential safety issues. Drinking or tap water did not show bioactive or toxic compounds, which was expected, whereas biogas or landfill water samples did. The hyphenated 2LabsToGo strategy is affordable and extremely useful for all laboratories with limited equipment but pressing challenges. It is ready to be used in various analytical tasks and applications.

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Thin-layer chromatogram development with a moving pipette delivering the mobile phase onto the surface of the adsorbent layer

Hałka-Grysińska

Skop

Klimek-Turek

et al. 2018

Journal of Chromatography A

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A new semiautomatic device with horizontal developing chamber for gradient thin-layer chromatography

Cited by 5 publications

References 16 publications

Rapid Purification and Formulation of Radiopharmaceuticals via Thin-Layer Chromatography

Rapid Purification and Formulation of Radiopharmaceuticals via Thin-Layer Chromatography

2LabsToGo─Recipe for Building Your Own Chromatography Equipment Including Biological Assay and Effect Detection

Thin-layer chromatogram development with a moving pipette delivering the mobile phase onto the surface of the adsorbent layer

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