A New RoVe Method to Purify Endophytic Fungi from Medicinal Plants Western Ghats of Karnataka India

Abstract

Endophytic fungi from medicinal plants were hardly ever reported as compared to those from soil and marine sources. The present results associate the presence of endophytic fungi in medicinal plants by isolating them from three medicinal plants i.e. Bombax ceiba, Aloe vera, and Ximenia americana. In the present research investigation, surface sterilization method and media were standardized, and 32 endophytic fungi were isolated from three medicinal plants. We assessed the competence of three different surface sterilization methods and four media for isolation of endophytic fungi. The RoVe method used was more effective in eliminating epiphytic microorganisms. Therefore by using new method we have isolated total of 32 fungal endophytes and those were belongs to Aspergillus nomius (63.20%), Aspergillus niger (41.60%), Thielaviopsis basicola (38.33%), Fusarium oxysporum (33.20%), Pestalotiopsis inflexa (27.20%), Nigrospora sphaerica (20%), Alternaria alternata (15.30%) and Phomopsis archeri (20%). This is the first report of successful isolation of endophytic fungi from the said medicinal plants, for using newly formulated surface sterilization method.

Brief Review on Thermophilic Bacteria and Their Applications

“…Moreover, thermozymes can be utilized as models for comprehension thermo soundness. Consequently, distinguishing underlying highlights associated with strength of thermozymes is fundamental for a hypothetical portrayal of the physico-compound standards adding to protein strength and collapsing [11,22]. Besides, this data is fundamental likewise for planning more steady proteins for modern cycles.…”

“…Totalled prion atoms are firmly impervious to traditional proteases and various techniques utilized for microbe inactivation, including autoclaving at 121 °C. Ongoing examinations have shown that amyloid type of prion can be corrupted by some microbial proteases using denaturing pre-treatments, like pre-warming and treatment with cleansers [21,22,28]. Promising wellspring of chemicals corrupting PrPSc without cleansers or under non-basic conditions are some thermophiles and microorganisms having a place with the family of Streptomyces [7,9,12,23,27,28].…”