A new rotating-disk sorptive extraction mode, with a copolymer of divinylbenzene and N-vinylpyrrolidone trapped in the cavity of the disk, used for determination of florfenicol residues in porcine plasma

Abstract

A novel extraction approach was developed based on rotating-disk sorptive extraction (RDSE). In this approach the rotating-disk extraction device consists of a Teflon disk, with a cavity that is loaded with a commercial sorbent phase selected according to the polarity of the analyte. To avoid leakage of the sorbent, the cavity is covered with a fiberglass filter and sealed with a Teflon ring. The proposed novel analytical RDSE technique was used in this study to determine florfenicol levels in plasma as a model analyte, or sample system, to describe the pharmacokinetics of a veterinary formulation. The sorbent used for this application was the copolymer of divinylbenzene and N-vinylpyrrolidone (Oasis HLB), which was selected because the florfenicol molecule contains both hydrophilic and lipophilic moieties. After the extraction, final determination of the analyte was performed by HPLC-DAD. Calibration plots and other analytical features were obtained after 90 min of extraction. The calibration plot was linear over the interval 0.4-16 μg mL(-1) (n = 6), with R (2) = 0.9999. Recovery and repeatability were determined using a blank plasma sample spiked with 4.8 μg mL(-1) florfenicol. A recovery of 91.5 %, with a relative standard deviation (RSD) of 8.8 %, was obtained when the extraction was evaluated using six different rotating-disk devices. Precision was also assessed, using the same disk (containing the same sorbent phase) for eight aliquots of the same sample. The RSD under these conditions was 10.2 %, clearly indicating that the sorptive phase could possibly be re-used. Accordingly, RDSE is a suitable sample preparation alternative to liquid-liquid extraction (LLE), solid-phase extraction (SPE), and stir-bar sorptive extraction (SBSE).

Determination of Triclabendazole in Cattle Plasma as Its Sulphoxide and Sulphone Metabolites by Rotating Disk Sorptive Extraction Combined With High-Performance Liquid Chromatography and Its Application to Pharmacokinetic Studies

“…The extraction device used in RDSE contains a cavity into which the sorptive phase is loaded to extract the analyte from complex samples. 13,14 By following this approach, in this work, we demonstrated that this extraction platform is equally applicable for determination of TCB as its metabolites sulphone and sulphoxide in cattle plasma. This method results in a greener and simpler alternative compared with methods based on SPE.…”

“…Taking into account our previous experiences in RDSE procedures, 13,14,18,19 the rotation velocity of the disk was kept at 2000 rpm, and all the optimization was made in absence of matrix modifiers. Rotation velocity was kept at the maximum velocity achieved by the magnetic stirrer, because under this condition the boundary layer formed on the surface of the sorptive phase decreases and analyte mass transfer is faster.…”

Green method to determine triazine pesticides in water using Rotating Disk Sorptive Extraction (RDSE)

“…As a result, no significant influence was obtained in the areas obtained for the analytes, which is why it was decided to work without the addition of NaCl. Other results indicate that the extraction of compounds of high and medium polarity increases in the presence of salts due to the salting out effect ( Cañas et al., 2014 ; Corrotea el at., 2016 ). The optimized values for the extraction of the seven triazines were pH: 8, Sample volume (mL): 25.0, elution volume (mL): 4.0, amount of adsorbent material (mg): 25, extraction time (min): 100, elution time (min): 5, stirring speed (rpm): 1200.…”

Recent Advances in Extraction and Stirring Integrated Techniques

“…Richter et al proposed an evolution of the classic RDSE in order to exploit this characteristic. In this case, a cavity is dug into the unit and finally loaded with polymeric sorbent (HLB) particles ( Figure 10B) [63]. The cavity is covered with a filter to confine the particles, avoiding their losses during the extraction.…”