A new reliable chemiluminescence immunoassay (CLIA) for prostaglandin E2 using enhanced luminol as substrate

Neupert, Werner; Oelkers, R; Brune, Kay; Geißlinger, Gerd

doi:10.1016/s0090-6980(96)00103-7

Cited by 15 publications

(8 citation statements)

References 38 publications

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“…For a long time, immunoassays (enzyme immunoassay (EIA) and radioimmunoassay (RIA)), due to their high sensitivity, were the most widely used quantitative techniques for oxylipins: RIA has been developed to quantify concentrations of IsoPs (8-iso-PGF2α) in human plasma and urine [26], 15-keto-dihydro-PGF2α in human plasma [62], Prostaglandin E2 (PGE2) and LTB4 in human prostate tissues [63], PGE2 in human plasma [64,65], PGE2, PGF2α, PGI2, 6-oxo-PGF2α, TXA2, TXB2 [66] and PGF2α, PGI2, TXA2, 13,14-dihydro-15-keto PGF2α (M-PGF2α), 6-keto PGF1α, and TXB2 [67] in human follicular fluid. ELISA, similar to EIA and RIA, requires specific antibodies, and due to the structural similarity of oxylipins, it is unlikely that antibodies that will sufficiently distinguish them can be obtained [68].…”

Section: Immunoassay Methodsmentioning

confidence: 99%

Methods of the Analysis of Oxylipins in Biological Samples

et al. 2020

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Oxylipins are derivatives of polyunsaturated fatty acids and due to their important and diverse functions in the body, they have become a popular subject of studies. The main challenge for researchers is their low stability and often very low concentration in samples. Therefore, in recent years there have been developments in the extraction and analysis methods of oxylipins. New approaches in extraction methods were described in our previous review. In turn, the old analysis methods have been replaced by new approaches based on mass spectrometry (MS) coupled with liquid chromatography (LC) and gas chromatography (GC), and the best of these methods allow hundreds of oxylipins to be quantitatively identified. This review presents comparative and comprehensive information on the progress of various methods used by various authors to achieve the best results in the analysis of oxylipins in biological samples.

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Section: Immunoassay Methodsmentioning

confidence: 99%

Methods of the Analysis of Oxylipins in Biological Samples

et al. 2020

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“…The CL properties of luminol are widely used as an analytical tool for environmental applications, immunoassays [20, 21], monitoring of metabolic pathways, detection of free radicals [22], analysis of a variety of trace metals, and forensic identification and detection of inorganic substances. Furthermore, luminol has been widely used in the area of pharmaceutical and life science industries.…”

Section: Introductionmentioning

confidence: 99%

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

Khan

Idrees

Moxley

et al. 2014

Appl Biochem Biotechnol

232

181

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Chemiluminescence (CL) is an important method for quantification and analysis of various macromolecules. A wide range of CL agents such as luminol, hydrogen peroxide, fluorescein, dioxetanes and derivatives of oxalate, and acridinium dyes are used according to their biological specificity and utility. This review describes the application of luminol chemiluminescence (LCL) in forensic, biomedical, and clinical sciences. LCL is a very useful detection method due to its selectivity, simplicity, low cost, and high sensitivity. LCL has a dynamic range of applications, including quantification and detection of macro and micromolecules such as proteins, carbohydrates, DNA, and RNA. Luminol-based methods are used in environmental monitoring as biosensors, in the pharmaceutical industry for cellular localization and as biological tracers, and in reporter gene-based assays and several other immunoassays. Here, we also provide information about different compounds that may enhance or inhibit the LCL along with the effect of pH and concentration on LCL. This review covers most of the significant information related to the applications of luminol in different fields.

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“…Covering a wide range of these highly bioactive compounds still remains a challenge with modern analytical techniques. As a common approach, immunoassays are performed, either as radioimmunoassay [15, 16], enzyme immunoassays [17], or luminescent immunoassays [18, 19]. They are specific and focus on one or only few compounds.…”

Section: Introductionmentioning

confidence: 99%

Quantitative profiling of oxylipins through comprehensive LC-MS/MS analysis: application in cardiac surgery

et al. 2012

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Oxylipins, including eicosanoids, affect a broad range of biological processes, such as the initiation and resolution of inflammation. These compounds, also referred to as lipid mediators, are (non-) enzymatically generated by oxidation of polyunsaturated fatty acids such as arachidonic acid (AA). A plethora of lipid mediators exist which makes the development of generic analytical methods challenging. Here we developed a robust and sensitive targeted analysis platform for oxylipins and applied it in a biological setting, using high performance liquid chromatography coupled to tandem mass spectrometry (HPLC-MS/MS) operated in dynamic multiple reaction monitoring (dMRM). Besides the well-described AA metabolites, oxylipins derived from linoleic acid, dihomo-γ-linolenic acid, α-linolenic acid, eicosapentaenoic acid and docosahexaenoic acid were included. Our comprehensive platform allows the quantitative evaluation of approximately 100 oxylipins down to low nanomolar levels. Applicability of the analytical platform was demonstrated by analyzing plasma samples of patients undergoing cardiac surgery. Altered levels of some of the oxylipins, especially in certain monohydroxy fatty acids such as 12-HETE and 12-HEPE, were observed in samples collected before and 24 h after cardiac surgery. These findings indicate that this generic oxylipin profiling platform can be applied broadly to study these highly bioactive compounds in relation to human disease.FigureLC-MS/MS chromatogram of 104 oxylipins on a Triple Quadrupole MS system employing dynamic MRM in the Negative Ion Electrospray modeElectronic supplementary materialThe online version of this article (doi:10.1007/s00216-012-6226-x) contains supplementary material, which is available to authorized users.

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A new reliable chemiluminescence immunoassay (CLIA) for prostaglandin E2 using enhanced luminol as substrate

Cited by 15 publications

References 38 publications

Methods of the Analysis of Oxylipins in Biological Samples

Methods of the Analysis of Oxylipins in Biological Samples

Luminol-Based Chemiluminescent Signals: Clinical and Non-clinical Application and Future Uses

Quantitative profiling of oxylipins through comprehensive LC-MS/MS analysis: application in cardiac surgery

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