A new ratiometric fluorescent detection of Glucose-6-phosphate dehydrogenase enzyme based on dually emitting carbon dots and silver nanoparticles

Behzadifar, Shakila; Pebdeni, Azam Bagheri; Hosseini, Morteza; Mohammadnejad, Javad

doi:10.1016/j.microc.2022.107947

Cited by 9 publications

(5 citation statements)

References 35 publications

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“…The corresponding linear range of G6PD was 0.1 to 1.0 × 10 4 U/L (Figure S7A,B) and the limit of detection was 0.03 U/L ( S / N = 3), which was significantly better than the results of previous reports (Table S3). In addition, the PEC method for G6PD was relatively simple to operate compared to the fluorescent, , colorimetric, , and electrochemical − methods that usually involve multi-step reactions. The photocurrent responses from some potential interferents were obtained to evaluate the selectivity of the assay (Figures D and S7C).…”

Section: Results and Discussionmentioning

confidence: 99%

Bioinduced Surface Oxygen Vacancies on Bismuth Trioxide Nanocrystals for Photocathodic Bioassays

Wang

Zhu

et al. 2023

ACS Appl. Nano Mater.

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Cathodic photoelectrochemistry with superior photostability and anti-interference capability has been shown to be appealing in the field of bioanalysis. However, a major challenge regarding this technology is the limited photoinduced electron transfer signal transduction mechanism. Herein, we disclose a finding of the bioinduced surface oxygen vacancy (V O ) on bismuth trioxide (Bi 2 O 3 ) nanocrystals, which underlies an innovative mechanism for cathodic photoelectrochemical (PEC) bioanalysis. The protocatechuic acid engendered from the tandem enzymatic reaction of p-hydroxybenzoate hydroxylase (PHBH) and glucose-6-phosphate dehydrogenase (G6PD) can coordinate with the surface of Bi 2 O 3 nanocrystals through forming binary Bi−O−C bonds, which breaks the initial Bi−O bonds and enables the escape of O 2− from the lattice to form surface V O in situ. The surface V O can function as a separation center for charge carriers, which is favorable to the generation of cathodic photocurrent. In such a system, the cathodic signal is linearly correlated with the targets, glucose-6-phosphate (G-6-P) and G6PD, in concentration ranges of 8.0 to 8.0 × 10 5 μM and 0.1 to 1.0 × 10 4 U/L, achieving detection limits of 2.0 μM and 0.03 U/L, respectively. This study not only offers a way of introducing surface V O in situ but also enriches the current toolbox of cathodic PEC bioassays and promises to stimulate further interest in exploring surface effect engineering in other fields.

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Section: Results and Discussionmentioning

confidence: 99%

Bioinduced Surface Oxygen Vacancies on Bismuth Trioxide Nanocrystals for Photocathodic Bioassays

Wang

Zhu

et al. 2023

ACS Appl. Nano Mater.

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“…The dual emission strategy is a versatile and effective approach that capitalizes on the intrinsic fluorescence properties of CDs. In this method, CDs are engineered or modified to emit light at two distinct wavelengths under the excitation of a single wavelength [ 64 , 66 , 67 , 74 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 ] ( Figure 4 ). These dual-emitting CDs offer a built-in reference signal, enhancing the accuracy and reliability of ratiometric measurements.…”

Section: Construction Strategies Of Cds In Detecting Environmental Co...mentioning

confidence: 99%

“…One of these emissions typically serves as the analyte-specific response signal, while the other serves as a constant reference signal. The reference signal remains unaffected by environmental changes, making it an ideal internal standard for calibration and quality control [ 64 , 67 , 103 , 104 , 105 , 106 , 107 ].…”

Section: Construction Strategies Of Cds In Detecting Environmental Co...mentioning

confidence: 99%

“…Biological and Biomolecular Targets: CD-based ratiometric fluorescence probes have been adapted for detecting specific biological and biomolecular targets, such as bacteria [ 82 , 163 , 164 ], biomarkers [ 75 , 165 ], cholesterol [ 77 ], amino acids [ 64 ], guanine [ 166 , 167 ], DNA [ 168 ] ( Figure 7 d,e), and proteins [ 1 , 91 , 107 , 169 , 170 ]. These probes find applications in genomics, molecular diagnostics, and bioanalytical assays.…”

Section: Application In Environmental Monitoringmentioning

confidence: 99%

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Advances in Carbon Dot-Based Ratiometric Fluorescent Probes for Environmental Contaminant Detection: A Review

Xing,

Wang,

Wang

2024

Micromachines

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Detecting environmental contaminants is crucial for protecting ecosystems and human health. While traditional carbon dot (CD) fluorescent probes are versatile, they may suffer from limitations like fluctuations in signal intensity, leading to detection inaccuracies. In contrast, ratiometric fluorescent probes, designed with internal self-calibration mechanisms, offer enhanced sensitivity and reliability. This review focuses on the design and applications of ratiometric fluorescent probes based on CDs for environmental monitoring. Our discussion covers construction strategies, ratiometric fluorescence principles, and applications in detecting various environmental contaminants, including organic pollutants, heavy metal ions, and other substances. We also explore associated advantages and challenges and provide insights into potential solutions and future research directions.

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“…Typically, researchers can perform both bioluminescence and fluorescence imaging without the assistance of staff with special expertise. There are many advantages to fluorescence imaging, including high-throughput screening for compound optimization, high sensitivity, and multi-color imaging, as well as its shortcomings, including lack of clinical translation and low-depth penetration [144,149,150]. Using fluorescence imaging techniques with polymeric nanoparticles, researchers could monitor drug pharmacokinetics and drug delivery [151].…”

Section: Fluorescence Imagingmentioning

confidence: 99%

Polymer-Based Nanostructures for Pancreatic Beta-Cell Imaging and Non-Invasive Treatment of Diabetes

et al. 2023

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Diabetes poses major economic, social, and public health challenges in all countries worldwide. Besides cardiovascular disease and microangiopathy, diabetes is a leading cause of foot ulcers and lower limb amputations. With the continued rise of diabetes prevalence, it is expected that the future burden of diabetes complications, early mortality, and disabilities will increase. The diabetes epidemic is partly caused by the current lack of clinical imaging diagnostic tools, the timely monitoring of insulin secretion and insulin-expressing cell mass (beta (β)-cells), and the lack of patients’ adherence to treatment, because some drugs are not tolerated or invasively administrated. In addition to this, there is a lack of efficient topical treatment capable of stopping the progression of disabilities, in particular for treating foot ulcers. In this context, polymer-based nanostructures garnered significant interest due to their tunable physicochemical characteristics, rich diversity, and biocompatibility. This review article emphasizes the last advances and discusses the prospects in the use of polymeric materials as nanocarriers for β-cell imaging and non-invasive drug delivery of insulin and antidiabetic drugs in the management of blood glucose and foot ulcers.

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A new ratiometric fluorescent detection of Glucose-6-phosphate dehydrogenase enzyme based on dually emitting carbon dots and silver nanoparticles

Cited by 9 publications

References 35 publications

Bioinduced Surface Oxygen Vacancies on Bismuth Trioxide Nanocrystals for Photocathodic Bioassays

Bioinduced Surface Oxygen Vacancies on Bismuth Trioxide Nanocrystals for Photocathodic Bioassays

Advances in Carbon Dot-Based Ratiometric Fluorescent Probes for Environmental Contaminant Detection: A Review

Polymer-Based Nanostructures for Pancreatic Beta-Cell Imaging and Non-Invasive Treatment of Diabetes

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