A new quassinoid from fruits of <i>Brucea javanica</i>

Su, Zhiwei; Hao, Jing; Xu, Zhifang; Huang, Riming; Zhang, Nenling; Qiu, Shaofu

doi:10.1080/14786419.2013.821119

Cited by 18 publications

(9 citation statements)

References 19 publications

(16 reference statements)

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“…javanica fruit and seed-derived extracts or commercially available oil emulsion have been investigated. (8,9,(11)(12)(13)(14)(15)(16) Currently, we investigated on BJLE as another possible potential source for anti-cancer agent. We found that treatment of BJLE reduced cell viability percentages in BJLE concentration dependent manner.…”

Section: Bjle Attenuated Mitochondrial Membrane Permeability (Mmp)mentioning

confidence: 99%

“…(3)(4)(5)(6)(7) B. javanica extract has been reported to have antiproliferative and cell death induction activities. (8)(9)(10)(11)(12)(13)(14)(15)(16) There were numerous cancer lines have been tested with B. javanica extract, including breast cancer cell lines, such as MCF-7 (8-10), HTB126 (9) and MDA-MB231 (11); cervical cancer cell lines, such as CaSki (9), C33A (9) and HeLa (9); pancreatic cancer cell lines, such as PANC-1 (12,13), SW1990 (12,13), CAPAN-1 (12,13) and CAPAN-2 (14); non-small cell lung cancer cell line, such as A549 (8,11); prostate cancer cell line, such as LNCaP (9); hepatic cancer cell line, such as Hep3B (11); oesophageal cancer cell line, such as SLMT-1 (11); bladder cancer cell line, such as T24 (15); colon cancer cell line, such as HCT116 (16); oral cancer cell lines, such as KB (17) and ORL-48 (17). All reports showed that B. javanica extract was cytotoxic for cancer cell lines.…”

Section: Introductionmentioning

confidence: 99%

“…All reports showed that B. javanica extract was cytotoxic for cancer cell lines. (8)(9)(10)(11)(12)(13)(14)(15)(16)(17) B. javanica extract was shown to have several mechanisms in inducing cell death. (9,(11)(12)(13)(14)(15) Apoptosis induction by B. javanica extract was reported, in both p53-dependent and -independent pathways.…”

Section: Introductionmentioning

confidence: 99%

“…Most of investigated B. javanica extracts were derived from seeds and fruits, or commercially available oil emulsion. (8,9,(11)(12)(13)(14)(15)(16) However, other parts of B. javanica are also resourceful, like leaves, twigs, fruits and stems. (1) However, these parts were not well investigated, only one report showing active compounds of B. javanica extract derived from twigs, leaves and and inflorescence.…”

Section: Introductionmentioning

confidence: 99%

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Brucea javanica Leaf Extract Induced Apoptosis in Human Oral Squamous Cell Carcinoma (HSC2) Cells by Attenuation of Mitochondrial Membrane Permeability

2015

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BACKGROUND: Brucea javanica extract has been reported to have anti-proliferative and cell death induction activities. B. javanica extract was reported to induce apoptosis through caspase cascade. Most of investigated B. javanica extracts were derived from seeds and fruits, or commercially available oil emulsion. Therefore we conducted a study on B. javanica leaf extract (BJLE) in oral cancer cells.METHODS: B. javanica leaves were collected, identified, minced, dried, extracted with distilled ethanol at room temperature for 24 hours, filtered and evaporated. Resulted BJLE was stored at 4°C. Human oral squamous cell carcinoma (HSC)-2 cells were fasted for 12 hours and treated with BJLE in various concentrations for 24 hours. Cells were then quantified with 3-(4,5-dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium bromide (MTT) assay, demonstrated with 4',6'-diamidino-2-phenylindole (DAPI) staining. To find out mitochondrial membrane permeability (MMP), mitochondrial membrane potential (ΔΨM) was analyzed.RESULTS: BJLE reduced percentage of viable HSC-2 cells in a concentration dependent manner. BJLE induced apoptosis in HSC-2 cells. With treatment of 50 μg/ml BJLE, fragmented nuclei were seen. ΔΨM of HSC-2 cells treated with 50 μg/ml BJLE were shifted to the left, meaning that BJLE induced reduction of ΔΨM and attenuation of MMP.CONCLUSION: Our results suggested that BJLE could induce apoptosis by attenuating MMP.KEYWORDS: Brucea javanica, leaf, apoptosis, HSC-2, MTT, DAPI, mitochondria, permeability

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Section: Bjle Attenuated Mitochondrial Membrane Permeability (Mmp)mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Brucea javanica Leaf Extract Induced Apoptosis in Human Oral Squamous Cell Carcinoma (HSC2) Cells by Attenuation of Mitochondrial Membrane Permeability

2015

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“…So, far, 72 compounds have been identified from these plants, including 52 quassinoids, nine triterpenoids, five glycosides and one monoterpenoid (Liu et al, 2011). Bruceine A is isolated from the seeds and fruit of B. javanica (L.) Merr (Kim et al, 2004;Liu et al, 2012;Su et al, 2013;Ye et al, 2015;Du et al, 2017) and has the molecular formula C 26 H 34 O 11 , a mass of 522.54 g/mol and a powder with bitter taste (NoorShahida et al, 2009;Liu et al, 2011). Bruceine A has insecticidal, antifeedant and growth inhibition activities against tobacco budworm (Heliothis virescens F), armyworm (Spodoptera frugiperda) (Klocke et al, 1985) and fourth instar larvae of Mexican beetles (Epilachna varivestis Mulsant) (Leskinen et al, 1984).…”

Section: Introductionmentioning

confidence: 99%

Larvicidal Activity of Bruceine A against Aedes aegypti and Toxicity on Vero Cells

Sutiningsih¹,

Mustofa²,

Satoto³

et al. 2018

OnLine Journal of Biological Sciences

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Vector control is still based on the use of chemical insecticides, which can cause death of nontarget animals, pollution and the emergence of vector resistance. This study aims to assess the larvicidal activity of bruceine A against larvae of Aedes aegypti and its cytotoxic activity against Vero cells. Extraction and isolation of bruceine A from the seeds of Brucea javanica (L.) Merr by method of Subeki. The purity of bruceine A isolate is determined by using a thin layer of chromatography and high performance liquid chromatography. Larvicidal activity of bruceine A on the larvae of A. aegypti from instar III until the beginning of instar IV was measured using a bioassay method. The examination of bruceine A cytotoxicity on Vero cells was performed by Micro-culture Tetrazolium assay (MTT). The results showed that mortality of A. aegypti larvae increased with increasing concentration of bruceine A. Log probit analysis of the larva mortality showed that the lethal concentration 50 and 90 (LC 50, LC 90) were 0.453±0.022 ppm and 4.962±0.681 ppm for 24 h respectively. The cytotoxic activity of bruceine A in Vero cells is low, with inhibitor concentration 50 (IC 50) values of 1251.324±0.162 µg/mL. Bruceine A has larvicidal activity against A. aegypti; therefore, it is a potential natural larvicide with low cytotoxicity.

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Comprehensive profiling and characterization of quassinoids from the seeds of Brucea javanica via segment and exposure strategy coupled with modified mass defect filter

et al. 2015

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Quassinoids, the predominant constituents in the seeds of Brucea javanica (BJ), have gained an increasing interest over the past decades since the discovery of their extensive biological activities. In the present study, a method based on the segment and exposure strategy coupled with two mass spectrometer data acquisition techniques was firstly developed and validated for comprehensive profiling of quassinoids in the seeds of BJ via high-performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (HPLC-QTOF/MS). The segment and exposure strategy could significantly improve the detection efficiency for trace quassinoids in the seeds of BJ. Furthermore, the five-point screening approach based on modified mass defect filter (MDF) and the visual isotopic ion technique could rapidly screen the precursor ions of interest. The fragmentation behavior of quassinoids was systematically investigated, and a total of 148 quassinoids including 86 potentially new ones were unambiguously or tentatively identified in the seeds of BJ. Collectively, our results demonstrate that the integrated strategy reported in this study has considerable potential for rapid screening of natural compounds especially for the trace ones in herbal medicines.

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A new quassinoid from fruits of Brucea javanica

Cited by 18 publications

References 19 publications

Brucea javanica Leaf Extract Induced Apoptosis in Human Oral Squamous Cell Carcinoma (HSC2) Cells by Attenuation of Mitochondrial Membrane Permeability

Brucea javanica Leaf Extract Induced Apoptosis in Human Oral Squamous Cell Carcinoma (HSC2) Cells by Attenuation of Mitochondrial Membrane Permeability

Larvicidal Activity of Bruceine A against Aedes aegypti and Toxicity on Vero Cells

Comprehensive profiling and characterization of quassinoids from the seeds of Brucea javanica via segment and exposure strategy coupled with modified mass defect filter

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