“…PAGs are usually extracted from plant material by polar solvents, such as water, [66] methanol, [67] ethanol, [25] or using a mixture of these solvents, [68] with or without heating [44,69] followed by fractionation by chromatography using Sephadex LH-20, Diaion HP-20 and/or silica gel using a wide range of solvents. [25,[67][68] Different analytical methods for the isolation and qualitative and quantitative determination of PAGs in plants include HSCCC (High Speed Counter Current Chromatography), [70] HPLC-UV (High Performance Liquid Chromatography with Ultraviolet Detection), [27] HPLC-PDA (High Performance Liquid Chromatography Coupled with Photo Diode Array Detector), [28] and LC-DAD-MS (Liquid Chromatography Coupled to Diode Array Detection and Mass Spectrometry) [29] among others. [30] The structural elucidation of some PAGs isolated from plants was performed indirectly by acidic hydrolysis of the glycosidic bond [64] followed by the analysis of the corresponding sugar and the polyacetylene units by optical rotation, [71] UV, [72] MS, [73] and 1D-NMR and 2D-NMR.…”