A NEW P^k PHENOTYPE IN THE P BLOOD GROUP SYSTEM

Abstract

Summary A healthy 22‐year‐old woman was noted to have erythrocytes of the Pk phenotype: a strong Pk antigen, no detectable P antigen and anti‐P antibody in her serum. Her erythrocytes contained four to six times as much Pk glycolipid (globotriaosylceramide or CTH) and approximately half as much P glycolipid (globotertraosylceramide or globoside) as normal red cells. The structures of CTH and globoside were characterized by analysis of permethylated sugars and complement fixation in addition to chromaographic mobility and sugar composition. Inasmuch as the erythrocytes of two Pk individuals that were analysed previously (Marcus et al., 1976) contained no detectable globoside, these abnormalities appear o represent a new phenotype in the P blood group system.

Glycosphingolipids of K562 cells: A chemical and immunological analysis

“…Standard neutral glycolipids and gangliosides (Table I) were prepared and characterized as described previously (Kundu et al, 1980a. Rabbit IgG antibodies to GSLs were isolated by affinity chromatography (Kundu et al, 1980b) and their specificity was determined by complement fixation (Naiki et al, 1975) or by a solid-phase radioimmunoassay (Karol et al, 1981).…”

A hemolytic anti‐LKE associated with a rare LKE‐negative, “weak P” red blood cell phenotype: alloanti‐LKE and alloanti‐P recognize galactosylgloboside and monosialogalactosylgloboside (LKE) antigens

“…Most examples of LKE-N described to date share features of the P k variant phenotype, with elevated Gb3/P k expression. 4,8 Our patient is the first known example of LKE-N arising from a weak P phenotype and is only the second example of weak P characterized in the literature. The first weak P was identified serendipitously in a normal donor after routine phenotyping showed weaker agglutination with some anti-PP1P k .…”

“…LKE‐negative (LKE‐N) is reported in 1% to 2% of P+ donors, as well as rare p and P k individuals who are deficient in complex globo‐GSLs. Interestingly, there is an apparent inverse relationship between LKE and P k , with many LKE‐N donors showing elevated P k expression reminiscent of a “P k variant” phenotype . LKE may also have interactions with other carbohydrate blood groups.…”

“…Interestingly, there is an apparent inverse relationship between LKE and P k , with many LKE-N donors showing elevated P k expression reminiscent of a "P k variant" phenotype. 4,7,8 tissues, LKE can be weakened in the presence of secretor. 9 Serologic studies with rare human anti-LKE also suggest an association between LKE-W, A1, and P2 phenotypes.…”

Sphingolipids: Modulators of HIV-1 Infection and Pathogenesis

“…Therefore, we further investigated the role of GM3 in HIV-1 entry by examining effect of GSL depletion from the p-lymphocytes, which do not express any Gb3 and the next upstream globoseries GSL, Gb4 due to absence of the enzyme, UDP galactose: lactosylceramide-galactosyl transferase (Hex3Cersynthase) (Marcus et al, 1976;Kijimoto-Ochiai et al, 1977;Kundu et al, 1978Kundu et al, , 1980Kundu et al, , 1981. However, p-lymphocytes express exceptionally high levels of the ganglioside GM3 , which is also the major ganglioside of normal lymphocytes (Kundu et al, , 1980Marcus et al, 1976Marcus et al, , 1981. Normal lymphocytes express Gb3 (P k antigen) as well as other neutral and acidic GSLs (Momota et al, 1982).…”