A new multiparameter flow cytometer: Optical and electrical cell analysis in combination with video microscopy in flow

Wietzorrek, Joachim; Plesnila, Nikolaus; Baethmann, A.; Kachel, Volker

doi:10.1002/(sici)1097-0320(19990401)35:4<291::aid-cyto1>3.0.co;2-y

Cited by 14 publications

(8 citation statements)

References 15 publications

(6 reference statements)

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“…Since the cell would not be able to be relocated, we would have to believe the data that has been stored. Confirmation of the morphology could be done by sophisticated cell sorting or new in-flow imaging technology only (36).…”

Section: Discussionmentioning

confidence: 99%

Scanning fluorescent microscopy analysis is applicable for absolute and relative cell frequency determinations

et al. 2004

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Background: Flow cytometry (FCM) and laser scanning cytometry (LSC) are the routine techniques for fluorescent cell analysis. Recently, we developed a scanning fluorescent microscopy (SFM) technique. This study compares SFM to LSC (two slide-based cytometry, SBC, techniques) and FCM, in experimental and clinical settings. Methods: For the relative cell-frequency determinations, HT29 colorectal cancer cells and Ficoll separated blood mononuclear cells (FSBMCs) were serially diluted (from 1:1 to 1:1,000) and measured by each of the three techniques. For the absolute cell number determinations (only for SBC) FSBMCs were smeared on slides, then HT29 cells were placed on the slide with a micromanipulator (5-50 cells). Tumor cells circulating in the peripheral blood were isolated by magnetic separation from clinical blood samples of colorectal cancer patients. All samples were double-stained by CD45 ECD and CAM5.2 FITC antibodies. For slides,

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Section: Discussionmentioning

confidence: 99%

Scanning fluorescent microscopy analysis is applicable for absolute and relative cell frequency determinations

et al. 2004

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“…Because of its capacity to collect information-rich data sets on thousands of cells, flow cytometry facilitates valuable insights into connections between single-cell and population-level processes not available with other techniques (66,86,119,208,243). Flow cytometers capable of sorting cells on the basis of their fluorescence characteristics or of simultaneous in-line video microscopy add to the versatility of this method (66,250). Flow cytometry has proven to be an invaluable resource in the study of apoptosis in mammalian cells (64).…”

Section: Cytometrymentioning

confidence: 99%

Single-Cell Microbiology: Tools, Technologies, and Applications

Brehm-Stecher

Johnson

2004

Microbiol Mol Biol Rev

449

316

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The field of microbiology has traditionally been concerned with and focused on studies at the population level. Information on how cells respond to their environment, interact with each other, or undergo complex processes such as cellular differentiation or gene expression has been obtained mostly by inference from population-level data. Individual microorganisms, even those in supposedly “clonal” populations, may differ widely from each other in terms of their genetic composition, physiology, biochemistry, or behavior. This genetic and phenotypic heterogeneity has important practical consequences for a number of human interests, including antibiotic or biocide resistance, the productivity and stability of industrial fermentations, the efficacy of food preservatives, and the potential of pathogens to cause disease. New appreciation of the importance of cellular heterogeneity, coupled with recent advances in technology, has driven the development of new tools and techniques for the study of individual microbial cells. Because observations made at the single-cell level are not subject to the “averaging” effects characteristic of bulk-phase, population-level methods, they offer the unique capacity to observe discrete microbiological phenomena unavailable using traditional approaches. As a result, scientists have been able to characterize microorganisms, their activities, and their interactions at unprecedented levels of detail

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“…Also a few hybrid microscope-FC were developed to obtain COBs information to mimic FC (29). Immunofluorescent techniques were applied on slide substrate (30) to obtain cell populations using laser scanning instrumentation.…”

Section: How We Differ From Previous Studiesmentioning

confidence: 99%

“Virtual flow cytometry” of immunostained lymphocytes on microscopic tissue slides: iHCFlow™ tissue cytometry

Cualing¹,

Zhong²,

Moscinski³

2006

Cytometry Part B Clinical

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Background: A method and approach is developed for fully automated measurements of immunostained lymphocytes in tissue sections by means of digital color microscopy and patent pending advanced cell analysis. The validation data for population statistic measurements of immunostained lymphocytes in tissue sections using tissue cytometry (TC) is presented. The report is the first to describe the conversion of immunohistochemistry (IHC) data to a flow cytometry-like two parameter dot-plot display, hence the technique is also a virtual flow cytometry. We believe this approach is a paradigm shift, as well as novel, and called the system iHCFlow

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A new multiparameter flow cytometer: Optical and electrical cell analysis in combination with video microscopy in flow

Cited by 14 publications

References 15 publications

Scanning fluorescent microscopy analysis is applicable for absolute and relative cell frequency determinations

Scanning fluorescent microscopy analysis is applicable for absolute and relative cell frequency determinations

Single-Cell Microbiology: Tools, Technologies, and Applications

“Virtual flow cytometry” of immunostained lymphocytes on microscopic tissue slides: iHCFlow™ tissue cytometry

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