“Virtual flow cytometry” of immunostained lymphocytes on microscopic tissue slides: <i>i</i>HCFlow™ tissue cytometry

Cualing, Hernani; Zhong, Eric; Moscinski, Lynn C.

doi:10.1002/cyto.b.20148

Cited by 12 publications

(2 citation statements)

References 36 publications

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“…This method automatically converts digital images of immunostained tissue to percentage positive, using a multithresholding, iterative bit slice identification of stained nuclei and unstained nuclei. The single cell count results correlate with both manual Ki-67 scoring and standardized tissue flow cytometry results (3). Briefly, thymic sections, previously immunostained as described, using Ki-67 AP-DAB (Mib-1, Ventana) by automated Ventana XT (Tucson, AZ, USA) are put on the stage (2).…”

Section: Methodsmentioning

confidence: 93%

Quantitative Morphological and Molecular Pathology of the Human Thymus Correlate With Infant Cause of Death

Lloyd¹,

Burke²,

Kalantarpour³

et al. 2014

technol innov

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The objective of this study was to investigate and quantify the morphological and molecular changes in the thymus for common causes of human infant death. Thymic architecture and molecular changes apparent in human infant head trauma victims were assessed by microscopy and quantified by image analysis of digital whole slide images. Thymuses from victims of SIDS and suffocated infants displaying normal thymus architecture were used for comparison. Molecular expression of proliferation and serotonin receptor and transporter protein markers was evaluated. Duplicate morphological and molecular studies of rodent thymuses were completed with both mouse and rat models. Quantification of novel parameters of digital images of thymuses from human infants suffering mortal head trauma revealed a disruption of the corticomedullary organization of the thymus, particularly involving dissolution of the corticomedullary border. A similar result was obtained for related mouse and rat models. The human thymuses from head trauma cases also displayed a higher percentage of Ki-67-positive thymocytes. Finally, we determined that thymus expression of the human serotonin receptor, and the serotonin transporter, occur almost exclusively in the thymic medulla. Head trauma leads to a disruption of the thymic, corticomedullary border, and molecular expression patterns in a robust and quantifiable manner.

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Section: Methodsmentioning

confidence: 93%

Quantitative Morphological and Molecular Pathology of the Human Thymus Correlate With Infant Cause of Death

Lloyd¹,

Burke²,

Kalantarpour³

et al. 2014

technol innov

View full text Add to dashboard Cite

show abstract

“…Although this assessment has been of blood and bone marrow smears, the automated immunostainer could, in theory, also be used to phenotype other fresh cell samples such as fine needle aspirates, tissue imprints and cytocentrifuge preparations 11. Further developments with this technology could include the utilisation of image analysis software to provide objective interpretation of the percentage of positive cells, and double staining for dual antigen expression 12. These technologies, currently under development, would provide objective “virtual flow cytometry” for cell smear staining.…”

Section: Discussionmentioning

confidence: 99%