A new molecular method for the rapid detection of a metamitron-resistant target site in Chenopodium album

Thiel, Heike; Kluth, Christian; Varrelmann, Mark

doi:10.1002/ps.1975

Cited by 8 publications

(16 citation statements)

References 13 publications

(16 reference statements)

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“…The ED 50 values from the bioassay experiment indicated that the Ra and Rm populations were resistant to metamitron, as they were clearly different from the S population (Table 1). The RI for the Rm population (2.95 ± 0.60) was in accordance with the ratio 3.85 obtained by Thiel et al , who used the same population 26. The atrazine‐resistant biotype Ra had a lower RI (1.75 ± 0.39).…”

Section: Resultssupporting

confidence: 88%

Absorption, translocation and metabolism of metamitron in Chenopodium album

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Mechant

Rubin

et al. 2011

Pest Management Science

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Section: Resultssupporting

confidence: 88%

Absorption, translocation and metabolism of metamitron in Chenopodium album

Aper

Mechant

Rubin

et al. 2011

Pest Management Science

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“…and is still successfully used in screens of plastid-encoded herbicide resistance (Thiel et al, 2010). However, the approach of combining physiological characterization with local sequence analysis suffers from limitations: First, the candidate gene needs to be predictable, which implies that its biochemical and physiological attributes are already well characterized.…”

Section: Introductionmentioning

confidence: 99%

Spontaneous Chloroplast Mutants Mostly Occur by Replication Slippage and Show a Biased Pattern in the Plastome of Oenothera

et al. 2016

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Spontaneous plastome mutants have been used as a research tool since the beginning of genetics. However, technical restrictions have severely limited their contributions to research in physiology and molecular biology. Here, we used full plastome sequencing to systematically characterize a collection of 51 spontaneous chloroplast mutants in Oenothera (evening primrose). Most mutants carry only a single mutation. Unexpectedly, the vast majority of mutations do not represent single nucleotide polymorphisms but are insertions/deletions originating from DNA replication slippage events. Only very few mutations appear to be caused by imprecise double-strand break repair, nucleotide misincorporation during replication, or incorrect nucleotide excision repair following oxidative damage. U-turn inversions were not detected. Replication slippage is induced at repetitive sequences that can be very small and tend to have high A/T content. Interestingly, the mutations are not distributed randomly in the genome. The underrepresentation of mutations caused by faulty double-strand break repair might explain the high structural conservation of seed plant plastomes throughout evolution. In addition to providing a fully characterized mutant collection for future research on plastid genetics, gene expression, and photosynthesis, our work identified the spectrum of spontaneous mutations in plastids and reveals that this spectrum is very different from that in the nucleus.

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“…(), polymorphic plants surviving herbicide treatment will probably eliminate susceptible chloroplasts and become insensitive to further herbicide treatment. Chloroplast polymorphism for the serine‐264 to glycine mutation was already confirmed in susceptible C. album ( Thiel et al ., ) and Senecio vulgaris populations (Frey et al ., ).…”

Section: Discussionmentioning

confidence: 99%

“…The DNA extraction with FTA card was performed according to the protocol described by Thiel et al . (). The PCR amplification of the highly conserved psbA gene was performed using the primers psbA‐up and psbA‐low, as described in Thiel et al .…”

Section: Methodsmentioning

confidence: 97%

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Differential sensitivity of Atriplex patula and Chenopodium album to sugar beet herbicides: a possible cause for the upsurge of A. patula in sugar beet fields

et al. 2018

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In the last decade, the prevalence of Atriplex patula as a weed in the Belgian sugar beet area has increased. Possible reasons for its expansion in sugar beet fields, besides a poor implementation of the low-dose phenmedipham/activator/soil-acting herbicide (FAR) system, might be low sensitivity or evolved resistance to one or more herbicides used in sugar beet. Dose-response pot bioassays were conducted in the glasshouse to evaluate the effectiveness of five foliar-applied sugar beet herbicides (metamitron, phenmedipham, desmedipham, ethofumesate and triallate) and three preplant-incorporated herbicides (metamitron, lenacil, dimethenamid-P) for controlling five Belgian A. patula populations. Local metamitron-susceptible and metamitron-resistant populations of Chenopodium album were used as reference populations. Effective dosages and resistance indices were calculated. DNA sequence analysis of the photosystem II psbA gene was performed on putative resistant A. patula populations. Overall, A. patula exhibited large intraspecific variation in herbicide sensitivity. In general, A. patula populations were less susceptible to phenmedipham, desmedipham, ethofumesate and triallate relative to C. album populations. Two A. patula populations bear the leucine-218 to valine mutation on the chloroplast psbA gene conferring low level to high level crossresistance to the photosystem II inhibitors phenmedipham, desmedipham, metamitron and lenacil. In order to avoid insufficient A. patula control and further spread, seedlings should preferentially be treated with FAR mixtures containing higher-than-standard doses of metamitron and phenmedipham/desmedipham and no later than the cotyledon stage.

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A new molecular method for the rapid detection of a metamitron-resistant target site in Chenopodium album

Abstract: The method allows exhaustive surveys screening C. album leaf or seed samples for the occurrence of the D1 Ser264Gly mutation to confirm or disprove metamitron resistance in the case of unsatisfactory control.

Cited by 8 publications

References 13 publications

Absorption, translocation and metabolism of metamitron in Chenopodium album

Absorption, translocation and metabolism of metamitron in Chenopodium album

Spontaneous Chloroplast Mutants Mostly Occur by Replication Slippage and Show a Biased Pattern in the Plastome of Oenothera

Differential sensitivity of Atriplex patula and Chenopodium album to sugar beet herbicides: a possible cause for the upsurge of A. patula in sugar beet fields

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