A new microdialysis-electrochemical device for in vivo simultaneous determination of acetylcholine and choline in rat brain treated with N-methyl-(R)-salsolinol

Zhu, Wei; An, Yarui; Zheng, Jiajia; Tang, Linlin; Zhang, Wen; Jin, Litong; Jiang, Lai

doi:10.1016/j.bios.2009.05.023

Cited by 26 publications

(4 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Endogenous cholinergic neurotransmission, which is mediated by ACh and choline (Zhu et al, 2009), is a strong candidate for enhancement by a7 nAChR PAMs. While a7 PAMs were reported to enhance the actions of ACh in a7 nAChRs expressing systems in vitro (Bertrand and Gopalakrishnan, 2007), the rapid clearance of ACh by ACh-sterases in the synaptic cleft makes choline, an ACh precursor and metabolite, a more likely candidate (Alkondon and Albuquerque, 2006).…”

Section: Introductionmentioning

confidence: 99%

In vivo pharmacological interactions between a type II positive allosteric modulator of α7 nicotinic ACh receptors and nicotinic agonists in a murine tonic pain model

Freitas

Negus

Carroll

et al. 2013

British J Pharmacology

View full text Add to dashboard Cite

BACKGROUND AND PURPOSEThe a7 nicotinic ACh receptor subtype is abundantly expressed in the CNS and in the periphery. Recent evidence suggests that a7 nicotinic ACh receptor (nAChR) subtypes, which can be activated by an endogenous cholinergic tone comprising ACh and the a7 agonist choline, play an important role in chronic pain and inflammation. In this study, we evaluated whether type II a7 positive allosteric modulator PNU-120596 induces antinociception on its own and in combination with choline in the formalin pain model. EXPERIMENTAL APPROACHWe assessed the effects of PNU-120596 and choline and the nature of their interactions in the formalin test using an isobolographic analysis. In addition, we evaluated the interaction of PNU-120596 with PHA-54613, an exogenous selective a7 nAChR agonist, in the formalin test. Finally, we assessed the interaction between PNU-120596 and nicotine using acute thermal pain, locomotor activity, body temperature and convulsing activity tests in mice. KEY RESULTSWe found that PNU-120596 dose-dependently attenuated nociceptive behaviour in the formalin test after systemic administration in mice. In addition, mixtures of PNU-120596 and choline synergistically reduced formalin-induced pain. PNU-120596 enhanced the effects of nicotine and a7 agonist PHA-543613 in the same test. In contrast, PNU-120596 failed to enhance nicotine-induced convulsions, hypomotility and antinociception in acute pain models. Surprisingly, it enhanced nicotine-induced hypothermia via activation of a7 nAChRs. CONCLUSIONS AND IMPLICATIONSOur results demonstrate that type II a7 positive allosteric modulators produce antinociceptive effects in the formalin test through a synergistic interaction with the endogenous a7 agonist choline. Abbreviations%MPE, percent maximum possible effect; i.pl

show abstract

Section: Introductionmentioning

confidence: 99%

In vivo pharmacological interactions between a type II positive allosteric modulator of α7 nicotinic ACh receptors and nicotinic agonists in a murine tonic pain model

Freitas

Negus

Carroll

et al. 2013

British J Pharmacology

View full text Add to dashboard Cite

show abstract

“…Electrodeposition of this polymer allows the creation of a membrane that rejects prominent molecular interferences that can be misidentified as H 2 O 2 . This membrane is widely used as a coating in both microdialysis samples [ 96 ] and electrochemical sensors [ 97 ]. Using an unmodified, uncoated microelectrode, a rapid increase in the DA concentration as a result of vesicular ejection was detected in addition to a small signal, which was presumably attributed to H 2 O 2 .…”

Section: In Vivo Applications Of Nano- and Microelectrodes In Biologi...mentioning

confidence: 99%

Nano- and Microsensors for In Vivo Real-Time Electrochemical Analysis: Present and Future Perspectives

et al. 2022

View full text Add to dashboard Cite

Electrochemical nano- and microsensors have been a useful tool for measuring different analytes because of their small size, sensitivity, and favorable electrochemical properties. Using such sensors, it is possible to study physiological mechanisms at the cellular, tissue, and organ levels and determine the state of health and diseases. In this review, we highlight recent advances in the application of electrochemical sensors for measuring neurotransmitters, oxygen, ascorbate, drugs, pH values, and other analytes in vivo. The evolution of electrochemical sensors is discussed, with a particular focus on the development of significant fabrication schemes. Finally, we highlight the extensive applications of electrochemical sensors in medicine and biological science.

show abstract

“…Various methods had been reported for ACh detection which includes matrix-assisted laser desorption ionization time-of-flight mass spectrometry [7], high performance liquid chromatography coupled to post-column chemiluminescence detection [8], gas chromatography mass spectrometry [9], capillary zone electrophoresis [10], potentiometry [11], colorimetric detection [12], optical detection [13], chemiluminescence [14,15], photoelectrochemistry [16] and enzymatic electrochemical biosensors [17]. These methods are complicated, timeconsuming and require expensive equipment that is not practical for widespread application.…”

Section: Introductionmentioning

confidence: 99%

Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholine

Mohammadi

Beitollahi

Tajik

2018

Micro and Nano Syst Lett

View full text Add to dashboard Cite

In the present study, a screen-printed electrode based-sensor with electrochemical detection was developed for rapid and sensitive determination of acetylcholine. At first, the screen-printed carbon electrode was modified by using a magnetic core shell and then was used for voltammetric determination of acetylcholine in ampoule, serum and urine samples. The electrochemical behaviour of acetylcholine at the modified electrode was investigated by cyclic voltammetry. The modified electrode displayed a decrease in the overpotential (ca. 130 mV) and an obvious increase in the peak current compared to the non-modified screen-printed electrode. The results indicated that modified screenprinted electrode enhanced electrocatalytic activity towards the oxidation of acetylcholine. Under optimized conditions, the limit of detection from the experiment of acetylcholine determination was 0.02 µM with acetylcholine concentration in range of 0.1-500.0 µM. The reproducibility of the measurements was tested by recording the responses for 50.0 µM acetylcholine with four different developed sensors prepared on the same manner and the same day. A relative standard deviation value of 4.3% was obtained. Finally, a recovery test is done as a part of accuracy evaluation of the system. which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

show abstract

A new microdialysis-electrochemical device for in vivo simultaneous determination of acetylcholine and choline in rat brain treated with N-methyl-(R)-salsolinol

Cited by 26 publications

References 39 publications

In vivo pharmacological interactions between a type II positive allosteric modulator of α7 nicotinic ACh receptors and nicotinic agonists in a murine tonic pain model

In vivo pharmacological interactions between a type II positive allosteric modulator of α7 nicotinic ACh receptors and nicotinic agonists in a murine tonic pain model

Nano- and Microsensors for In Vivo Real-Time Electrochemical Analysis: Present and Future Perspectives

Nonenzymatic coated screen-printed electrode for electrochemical determination of acetylcholine

Contact Info

Product

Resources

About