A new method to destruct targeted cells using magnetizable beads and pulsed magnetic force

Ogiue-Ikeda, Mari; Sato, Yukiko; Ueno, Shinji

doi:10.1109/tnb.2003.820276

Cited by 29 publications

(19 citation statements)

References 13 publications

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“…Magnetic particle twisting was used to investigate the mechanical integrity and visco-elastic properties of the cytoskeleton (Moller et al 2003) and, in combination with optical tweezers, superparamagnetic beads were at the basis of force and torque measurement systems on single cells (Romano et al 2003). Recently, cell-bound magnetic microparticles, subjected to 0.1 ms magnetic field pulses, have been used to destruct the targeted cells by penetration of the beads into the cells or by rupturing the cells with the beads (Ogiue- Ikeda et al 2003), a nonthermal process which is different from hyperthermia.…”

Section: Cell Biophysicsmentioning

confidence: 99%

Magnetic bead handling on-chip: new opportunities for analytical applications

Gijs

2004

Microfluid Nanofluid

496

555

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This review describes recent advances in the handling and manipulation of magnetic particles in microfluidic systems. Starting from the properties of magnetic nanoparticles and microparticles, their use in magnetic separation, immuno-assays, magnetic resonance imaging, drug delivery, and hyperthermia is discussed. We then focus on new developments in magnetic manipulation, separation, transport, and detection of magnetic microparticles and nanoparticles in microfluidic systems, pointing out the advantages and prospects of these concepts for future analysis applications.

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Section: Cell Biophysicsmentioning

confidence: 99%

Magnetic bead handling on-chip: new opportunities for analytical applications

Gijs

2004

Microfluid Nanofluid

496

555

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“…Aggregation and selective uptake has been used to destroy cells via the application of pulsed magnetic fields and subsequent rupture of cells [205].…”

Section: Other Applicationsmentioning

confidence: 99%

Nanobiomagnetics

Leslie‐Pelecky¹,

Labhasetwar²,

Kraus³

2006

Advanced Magnetic Nanostructures

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The application of nanomagnetic materials to biological systems has produced significant advances in research, diagnosis, and treatment of numerous pathologies. This chapter summarizes the major applications of magnetic materials: magnetic targeting, drug and gene delivery, magnetic separation, the use of magnetic beads in manipulating single molecules, as contrast agents in magnetic resonance imaging, and for hyperthermia. Biocompatibility requirements for magnetic materials used in these applications are reviewed.

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“…The principle behind the magnetic-bead-based DNA extraction process is to use the magnetic beads labeled with a specific antibody to bind with the target cells in the bio-samples, followed by lysing the target cells and extracting the DNA by switchable charges, or using DNA probe-bound magnetic beads, to bind with the target DNA sample. Thus, the bound DNA samples can be extracted and purified by these processes (Ogiue-Ikeda et al 2003;Lawson et al 2006;Parham et al 2007). For example, a droplet-based DNA purification platform by using the magnetic particles from a lysed cell sample solution was reported (Lehmann et al 2006).…”

Section: Introductionmentioning

confidence: 99%

Extraction of genomic DNA and detection of single nucleotide polymorphism genotyping utilizing an integrated magnetic bead-based microfluidic platform

Lien

Liu

Lin

et al. 2008

Microfluid Nanofluid

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This study presents a new magnetic bead-based microfluidic platform, which integrates three major modules for rapid leukocytes purification, genomic DNA (gDNA) extraction and fast analysis of genetic gene. By utilizing microfluidic technologies and magnetic beads conjugated with CD 15/45 antibodies, leukocytes in a human whole blood sample can be first purified and concentrated, followed by extraction of gDNA utilizing surface-charge switchable, DNA-specific, magnetic beads in the lysis solution. Then, specific genes associated with genetic diseases can be amplified by an on-chip polymerase chain reaction (PCR) process automatically. The whole pretreatment process including the leukocytes purification and gDNA extraction can be performed in an automatic fashion with the incorporation of the built bio-separators consisting of microcoils array within less than 20 min. The detection of single nucleotide polymorphism (SNP) genotyping of methylenetetra-hydrofolate reductase (MTHFR) C677T region associated with an increased risk of genetic diseases was further performed to demonstrate the capability of the proposed system. The extracted gDNA can be transported into a micro PCR chamber for on-chip fast nucleic acid amplification of detection genes with minimum human intervention. Hence, the developed system may provide a powerful automated platform for pretreatment of human leukocytes, gDNA extraction and fast analysis of genetic gene.

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A new method to destruct targeted cells using magnetizable beads and pulsed magnetic force

Cited by 29 publications

References 13 publications

Magnetic bead handling on-chip: new opportunities for analytical applications

Magnetic bead handling on-chip: new opportunities for analytical applications

Nanobiomagnetics

Extraction of genomic DNA and detection of single nucleotide polymorphism genotyping utilizing an integrated magnetic bead-based microfluidic platform

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