This research presents the results of investigation of laser polarization°uorescence of biological layers (histological sections, cytological smears) in the task of diagnostics and di®er-entiation of early stages of cancer: Dysplasia -cervical microinvasive carcinoma of cervix uteri. The analytical conditions of polarization-optimal probing of biological layers were determined basing on the model of linear birefringence and dichroism of birefringent (¯brillar, porphyrin) networks. The technique of polarization-variable laser auto°uorescence was developed and experimentally tested. The objective criteria (statistical moments) of di®erentiation of histological sections auto°uorescent images of endometrium biopsy and cytological smears of it mucous coat were de¯ned. The operational characteristics (sensitivity, speci¯city, accuracy) of this technique were determined concerning the positions of probative medicine, and clinical e±ciency.