A New Method for Sampling Maternal Blood in the Placental Intervillous Space

Abstract

The intervillous space (IVS) is part of the histofunctional unit of the human placenta and by being a fetal-maternal exchange interface is an important subject of study for the understanding of fetal physiology, especially in nutritional investigations. A method developed for the collection of IVS blood has permitted to evaluate the fetalmaternal exchanges in an effective manner. Two disadvantages of this method, however, are the mixing of IVS blood and fetal blood and marked hemolysis. In the present study we introduce and describe some modifications of this method using a single stylet for the perforation of the chorionic plate which simplifies collection and reduces the chance of mixing and hemolysis of the samples obtained.

Effect of type 2 diabetes mellitus on the pharmacokinetics and transplacental transfer of nifedipine in hypertensive pregnant women

“…Blood was collected from the umbilical cord only after the cord had been clamped and sectioned, and the placenta expelled, with no risk to mother or neonate. Blood samples were collected from the umbilical artery and vein, and from the intervillous space, according to the technique described by Camelo Júnior et al .…”

Transplacental Distribution of Lidocaine and Its Metabolite in Peridural Anesthesia Administered to Patients With Gestational Diabetes Mellitus

“…Maternal blood samples were collected into heparinized syringes at the time of delivery and, after placental expulsion, blood samples were obtained from the umbilical artery and vein and from the intervillous space according to the technique described by Camelo et al 12 in order to determine lidocaine and MEGX concentrations and the rate of placental transfer of the drug.…”

Distribution of Bupivacaine Enantiomers and Lidocaine and Its Metabolite in the Placental Intervillous Space and in the Different Maternal and Fetal Compartments in Term Pregnant Women

“…Blood from the placental intervillous space was collected by the method of Meirelles and Matheus, modified by Camelo et al 8 After placental detachment, the retroplacental clot was removed, and the basal plate was closed with the membranes. The placenta was placed inside a plastic bag, which was lifted to a height that would permit the investigator to observe it, with the chorial plate looking down, and a region of the chorial plate with no fetal vessels was identified.…”