A new method for recording surface compound potentials in sympathetic ganglia from mouse, rat, and guinea pig—application to muscarinic and nicotinic depolarizations

Schroff, Karl-Christian; Aschhoff, Susanne; Schulze, Johannes; Nägele, Ursula; Remien, Jörg

doi:10.1016/s1056-8719(99)00040-4

Cited by 3 publications

(2 citation statements)

References 25 publications

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“…In the present experimental scheme, we used acetylcholine as cholinergic agonist because it is considered to be the classical preganglionic neurotransmitter of the sympathetic ganglionic pathway (Eccles & Libet 1961, Berthoud & Powley 1993. Carbacol was not used because it is a synthetic cholinergic agonist that, though more stable than acetylcholine, has activity only on a specific type of cholinergic receptors (Schroff et al 1999), and our purpose in this work is always to emulate in an ex vivo system of the physiological conditions. Besides, the use of carbacol in other experimental schemes has shown a response with the physiological agonist higher than expected (Miller et al 1997, Ghisdal et al 2005).…”

Section: Discussionmentioning

confidence: 99%

The cholinergic influence on the mesenteric ganglion affects the liberation of ovarian steroids and nitric oxide in oestrus day rats: characterization of an ex vivo system

Orozco¹,

Sosa²,

Fillipa³

et al. 2006

Journal of Endocrinology

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The axons that constitute the ovarian nervous plexus originate mostly in the principal neurons of the superior mesenteric ganglion (SMG) that is part of the sympathetic ganglionic chain and exhibits cholinergic receptors. In order to observe the effect of acetylcholine, the main neurotransmitter in the ganglionic transmission, the purpose of the present work was: first, to standardize an integrated ex vivo superior mesenteric ganglion-ovarian nervous plexus-ovary (SMG-ONP-O) system in oestrus day rats; secondly, to determine if the ganglionic cholinergic stimulus modifies the release of nitric oxide and steroids in the ovary compartment in the absence of humoral factors; and thirdly, to investigate if there are differences in the responses between the left and right ovaries caused by the neural stimulus. The ex vivo experimental left and right systems were developed and standardized. The systems were incubated in Krebs-Ringer phosphate buffer in a Dubnoff metabolic shaker. The progesterone release was determined to standardize the incubation times, obtaining different responses between the left and right systems, which shows that both systems have their own autonomic tone. Non-specific stimulation with KCl in the ganglion compartment provoked different responses in terms of release of progesterone and oestradiol. Progesterone decreased in the left and right systems.However, oestradiol diminished at short times and increased at 60 and 120 min in the left ovary, whereas it increases at 30 and 60 min in the right ovary. These different responses show the sensitivity and viability of both systems. When acetylcholine was used in the ganglion compartment, the release of nitric oxide, progesterone, androstenedione and oestradiol was evaluated. The liberation of nitrite increased at 15, 30 and 60 min in the left system and decreased in the right system at 120 min. Progesterone showed a decrease in its release at 15, 30 and 120 min and androstenedione at 15 min in the left ovary compartment. In the right ovary, only progesterone decreased in relation to the control at 120 min while androstenedione did not show significant changes. Oestradiol showed an increase in the left ovary compartment at all the studied times, while in the right ovary it did not show any changes. These results indicate that the neural stimulus from the superior mesenteric ganglion through the ovarian nervous plexus is one of the factors modulating the secretory activity of the ovarian steroids and nitric oxide. The system is viable and also shows a different sensitivity of the left ovary in relation to the right one at least in this cycle stage, characterized by marked irrigation and profound structural changes in the ovary.

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Section: Discussionmentioning

confidence: 99%

The cholinergic influence on the mesenteric ganglion affects the liberation of ovarian steroids and nitric oxide in oestrus day rats: characterization of an ex vivo system

Orozco¹,

Sosa²,

Fillipa³

et al. 2006

Journal of Endocrinology

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“…Similarly, afferent input to the ganglia, arising from cardiac and extracardiac anatomical structures, affects the electrical activity of various neuronal populations in the ganglia. These conclusions derive from data accumulated in electrophysiological animal studies performed in vitro [12,24], in situ [4,5,9,13] and in vivo [3,8], which examined the autonomic ganglia activity in relation to cardiovascular and respiratory functions. In these studies, the electrical activity obtained from individual autonomic ganglia cells was recorded using intracellular microelectrodes [3,8,9,13].…”

Section: Discussionmentioning

confidence: 99%

In vivo extracellular recording of sympathetic ganglion activity in a chronic animal model

Lavian

Kopelman

Shenhav

et al. 2003

Clinical Autonomic Research

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Surgery of the sympathetic system is performed for a variety of indications, hyperhidrosis being a major one. Despite excellent results, sympathectomy for hyperhidrosis bears a number of sequels, some of which may be devastating. Several surgical methods, empirically advocated to alleviate these problems, have only limited success. Chronic in vivo recording of the electrical activity from sympathetic ganglia may assist in understanding and clarifying complex problems of sympathetic surgery; however, no suitable method has been reported. An electrode device developed by our group was implanted on the stellate ganglion, in a chronic animal model (dog). The signals obtained were amplified, filtered, and transmitted via an A/D interface to be acquired and saved on a computer, using special software which we developed. Our method enabled the separate recording of neuroelectrical signals, ECG, and respiration waves. An additional software program, also developed by our group, was used to analyze the data. This chronic animal model allows investigation of surgical and pharmacological manipulations of the sympathetic system.

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Clindamycin and nicotinic neuromuscular transmission

Schulze

Toepfer

et al. 1999

The Lancet

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A new method for recording surface compound potentials in sympathetic ganglia from mouse, rat, and guinea pig—application to muscarinic and nicotinic depolarizations

Cited by 3 publications

References 25 publications

The cholinergic influence on the mesenteric ganglion affects the liberation of ovarian steroids and nitric oxide in oestrus day rats: characterization of an ex vivo system

The cholinergic influence on the mesenteric ganglion affects the liberation of ovarian steroids and nitric oxide in oestrus day rats: characterization of an ex vivo system

In vivo extracellular recording of sympathetic ganglion activity in a chronic animal model

Clindamycin and nicotinic neuromuscular transmission

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