A new method for fast isolation of insect antifeedant compounds from complex mixtures

Escoubas, Pierre; Fukushi, Yukiharu; Lajide, Labunmi; Mizutani, Junya

doi:10.1007/bf02751106

Cited by 33 publications

(27 citation statements)

References 19 publications

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“…Skimmia japonica has been screened for antifeedants using Spodoptera exigua, but only furanocoumarins were reported to be responsible for the deterrent properties of the plant (Escoubas et al, 1992). Our results indicate that furoquinolines also contribute to these effects.…”

Section: Discussionsupporting

confidence: 52%

Effects of furoquinoline alkaloids on the growth and feeding of two polyphagous lepidopterans

2007

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The furoquinoline alkaloids skimmianine and dictamnine were tested for effects on the feeding and growth of larvae of the generalist lepidopteran, Spodoptera litura. Skimmianine was further investigated for toxic and phototoxic effects on the larvae of Trichoplusia ni, also a generalist species.In feeding experiments with S. litura, growth and consumption of larvae decreased with increasing concentration of furoquinolines; skimmianine caused a greater reduction in growth than dictamnine. In T. ni, dietary skimmianine reduced growth and consumption; when administered topically, it significantly reduced consumption but without a concomitant reduction in growth. Phototoxicity of skimmianine was not apparent in T. ni because UV light failed to increase the negative effects of the alkaloid on larvae.

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Section: Discussionsupporting

confidence: 52%

Effects of furoquinoline alkaloids on the growth and feeding of two polyphagous lepidopterans

2007

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“…It has to be pointed out that the obtained results induce to the occurrence of synergic effect between the substances, so that each single compound or compound class presents bioactivity, which is magnified when the compounds are present in the same sample. Synergic effects between terpenes and terpenoids have already been reported by Escoubas et al 48 and Iscan et al, 49 among other authors.…”

mentioning

confidence: 54%

Screening of the odour-activity and bioactivity of the essential oils of leaves and flowers of Hyptis Passerina Mart. from the Brazilian Cerrado

Zellner¹,

Amorim²,

Miranda³

et al. 2009

J. Braz. Chem. Soc.

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A composição química dos óleos essenciais das folhas e flores de Hyptis passerina Mart., uma espécie rara do cerrado brasileiro, está sendo descrita pela primeira vez. A análise por CG-EM revelou sesquiterpenos como constituintes majoritários. β-epi-acorenol (35.7% e 32.8%, respectivamente no óleo essencial das folhas e flores) foi isolado e identificado por RMN uni e bidimensionais. O óleo das flores apresentou maior concentração de monoterpenos (hidrocarbonetos e oxigenados), enquanto que o óleo das folhas foi mais rico em diterpenos. O aroma dos óleos foi investigado por análise olfatométrica direta e por CG-EM-O. Foi observado, para ambos os óleos, aroma herbáceo, com o óleo das folhas contendo nuances aromáticas de verde, cozido e madeiroso, lembrando chá, enquanto o óleo das flores apresentou aspectos de aroma condimentado, madeiroso e mentolado. β-epi-acorenol, espatulenol, β-cariofileno e óxido de cariofileno mostraram-se relevantes para a atividade odorífera dos óleos, bem como, constituintes minoritários como o linalol. A atividade antimicrobiana dos óleos foi investigada pelos métodos de difusão em disco de agar e bioautografia de contato contra bactérias Gram-positivas, negativas e fungos. Os óleos apresentaram-se ativos contra os microorganismos com nível de inibição significativo.The chemical profile of the essential oils obtained from the leaves and flowers of Hyptis passerina Mart., a rare species of the Brazilian Cerrado, has been determined for the first time. Analyses by GC-MS showed sesquiterpenes as major compounds. β-epi-acorenol (35.7% and 32.8%, respectively from leaf and flower essential oils), was isolated and identified by 1D and 2D NMR. The flower-derived oil presented a higher concentration of hydrocarbon and oxygenated monoterpenes, while the leaf-oil was richer in diterpenes. The global odour impressions of both oils were given by direct analysis and GC-MS-O and were characterized as herbaceous with tea notes, and green, cooked and woody impressions for leaf-oil; herbaceous, with spicy, woody and minty notes for flower-oil. β-epi-acorenol, spathulenol, β-caryophyllene, and caryophyllene oxide were relevant for the odour-activity of both oils, as well as minor constituents, such as linalool. The antimicrobial activity was investigated by means of agar diffusion disc method and contact bioautography, against Gram-positive and negative bacteria and yeast. Both oils presented to be bioactive against the tested microorganisms with significant inhibition level.

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“…Our method presented here is not unique in its use of TLC plates (Metcalf et al 1980;Escoubas et al 1992) or other artificial substrates for Coleoptera (Nawrot et al 1991;Hare 1998). However, the short test period and the low demand of test chemicals make it a microbioassay, in line with the trend toward downsizing .chemical methods in chemical ecology.…”

Section: Discussionmentioning

confidence: 92%

“…Metcalf et al (1980) described feeding stimulation by direct testing of curcurbitacins separated on TLC (thin layer chromatography) plates. Escoubas et al (1992) described a feeding assay for insect larvae, including direct testing of chromatographic separations on TLC plates for antifeedant compounds.…”

Section: Introductionmentioning

confidence: 99%

A new microassay for antifeedants in Hylobius pine weevils (Coleoptera)

2004

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A micro-feeding assay was developed for testing small amounts of synthetics and extracts as antifeedants for Hylobius pine weevils. We devised a simple setup consisting of 5·5-mm pieces of thin layer chromatography cellulose plates used in pairs for a bioassay involving choice. After application of 1.5 ll solutions and evaporation of the solvent, each plate received a 5 ll aliquot of 1 M sucrose as a feeding stimulant. The test period was 4 h at 25°C with 6 days of starvation before the test, compared to just the 1 day sufficient for twig tests. Deprivation of water for 1 day at 65% RH before the test was critical because it increased feeding but not mortality. The combination of starvation and thirst reduced the relative variance (coefficient of variation) to below 50%. There was a good correlation of antifeedant activity in the microassay with the activity in a standard twig test. The microassay needs only several mg for a test series, whereas the twig test needs grams.

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A new method for fast isolation of insect antifeedant compounds from complex mixtures

Cited by 33 publications

References 19 publications

Effects of furoquinoline alkaloids on the growth and feeding of two polyphagous lepidopterans

Effects of furoquinoline alkaloids on the growth and feeding of two polyphagous lepidopterans

Screening of the odour-activity and bioactivity of the essential oils of leaves and flowers of Hyptis Passerina Mart. from the Brazilian Cerrado

A new microassay for antifeedants in Hylobius pine weevils (Coleoptera)

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